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The map label for this gene is lasB [H]

Identifier: 117921122

GI number: 117921122

Start: 3204926

End: 3206428

Strand: Reverse

Name: lasB [H]

Synonym: Shewana3_2681

Alternate gene names: 117921122

Gene position: 3206428-3204926 (Counterclockwise)

Preceding gene: 117921128

Following gene: 117921112

Centisome position: 64.49

GC content: 49.63

Gene sequence:

>1503_bases
ATGAAAGTTTATCTCTTACCTTCAGCAGTGGCGGTCTGTATTGCGGCGAGTCTTCAGGCGCAAGCAGTCGAAATCAAAGT
GAATCACGGGCAATCCCTCTCGGCAGCGGCTGGGTTAAGCCAACAACACAGCTTTAAAGCGATCAAAACGGTCACGTTTA
ATAAAGGTCAAACCAAAACAAAAGCGTTGCAATATTTTAATGGCGTACTCGTATATGGGCATCATCTGGTGGTGAGTAAC
GATGATAGCGAGCAGAGTGCGATTGGGGATGTGGCACAAATTGCGGATGATTTTTCGGTAGAGGCAGGGATTTCTCAAGG
GCAGGCGATTGCGGCTTTGAATCGGTTATATCCAGGTTCGGCGGCAACGGGAAAAAAGGATGTTGAGCTAGTGATCCTGA
TGCAGGACGATACCCCGAGGTTAGTGTATCGGGTGTCCTATTTAAGCGACCAAACGGATGCACTGTCTCGGCCAACGGGC
TTTGTCGATGCGCAAACTGGCGAGGTGCTTGAGCATTGGAATGAAATCATGACCGCCAAAAATAACGTCGGTAAACCCGG
TAGTGGTGGAGGTGGTTCAGGTGGCAGTAGCAATCCCTTTGATGCAACTGGCCCTGGCGGCAATGCCAAAATCGGGGATT
ACTATTACGGCAGTGATTACGGTTTTCTTAAGGTGAGTGAGTCTAATGGTACATGCACTATGACCAACGCCAACGTGAAA
ACCGTGAACATGGCGAACTCGACCCGCCGTGGCTCGGTGTTCACCTTTACTTGCCCAGAGAACACTTATCAGACGGTCAA
TGGCGCTTATTCGCCTTTAAATGATGCCCACTATTTCGGTGGCGTTATTTTTAATATGTATAAGGATTGGTATAACACAG
CGCCGTTAACCTTTCAGTTAGAGATGCGGGTGCATTATGGCCGTAACTATGAAAATGCCTTCTGGGATGGCTCTGCCATG
TCCTTCGGTGACGGTGCCAGTTACTTCTATCCCCTCGTGAGTTTGGATGTTTCCGCCCATGAAGTGAGCCACGGTTTTAC
CGAGCAAAACTCAGGCTTAAATTACAGTGGCCAGTCCGGCGGTATCAATGAGGCGTTTTCGGATATTGCCGGTGAAGCGG
CCGAGTTCTACATGCGTGGCAGTAACGACTGGAAAGTGGGTTACGACATAGTTAAAGGTAATGGCGCACTGCGTTATATG
GATAATCCGACTAAGGATGGCCGCTCAATCGACAATGCCGCCAACTACACTAGTTCCATGGATGTGCATTACTCCTCAGG
GGTGTTTAACAAAGCCTTTTATACGCTGGCGACTAAGAATGGTTGGGATACCCGCAAGGCGTTCGATGTGTTTGTGTTAG
CGAACCAGCTTTATTGGACACCGACCTCGACCTTTAACAAAGCGGCCTGCGGGGTTCAGCAGGCGGCGAGTGCCAAAGGT
TCTGGTTATCCAGTGGCGGATGTGGCTGCGGCATTTTTAGCGGTAGGGGTTATCTGCCAGTAA

Upstream 100 bases:

>100_bases
TAACTTTGTTTTAACAAGTGAGAAGGGTGCAGATTGAAGCCAAATTTTGTTCCCTTAAGTGTCATTGGTTCCTCTGTGGC
CTATGGCAAAGGATATCGCG

Downstream 100 bases:

>100_bases
TCTGGTTATTAGTGCAGCGATTTGATGACAATTAAGCCGTAAGCCTAAAACCGTTTCTAAGTTCCAGTTTCCAAGTTCTA
ATTTCGAAGCCCCTTAATGG

Product: vibriolysin

Products: NA

Alternate protein names: Elastase; Neutral metalloproteinase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MKVYLLPSAVAVCIAASLQAQAVEIKVNHGQSLSAAAGLSQQHSFKAIKTVTFNKGQTKTKALQYFNGVLVYGHHLVVSN
DDSEQSAIGDVAQIADDFSVEAGISQGQAIAALNRLYPGSAATGKKDVELVILMQDDTPRLVYRVSYLSDQTDALSRPTG
FVDAQTGEVLEHWNEIMTAKNNVGKPGSGGGGSGGSSNPFDATGPGGNAKIGDYYYGSDYGFLKVSESNGTCTMTNANVK
TVNMANSTRRGSVFTFTCPENTYQTVNGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENAFWDGSAM
SFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLNYSGQSGGINEAFSDIAGEAAEFYMRGSNDWKVGYDIVKGNGALRYM
DNPTKDGRSIDNAANYTSSMDVHYSSGVFNKAFYTLATKNGWDTRKAFDVFVLANQLYWTPTSTFNKAACGVQQAASAKG
SGYPVADVAAAFLAVGVICQ

Sequences:

>Translated_500_residues
MKVYLLPSAVAVCIAASLQAQAVEIKVNHGQSLSAAAGLSQQHSFKAIKTVTFNKGQTKTKALQYFNGVLVYGHHLVVSN
DDSEQSAIGDVAQIADDFSVEAGISQGQAIAALNRLYPGSAATGKKDVELVILMQDDTPRLVYRVSYLSDQTDALSRPTG
FVDAQTGEVLEHWNEIMTAKNNVGKPGSGGGGSGGSSNPFDATGPGGNAKIGDYYYGSDYGFLKVSESNGTCTMTNANVK
TVNMANSTRRGSVFTFTCPENTYQTVNGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENAFWDGSAM
SFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLNYSGQSGGINEAFSDIAGEAAEFYMRGSNDWKVGYDIVKGNGALRYM
DNPTKDGRSIDNAANYTSSMDVHYSSGVFNKAFYTLATKNGWDTRKAFDVFVLANQLYWTPTSTFNKAACGVQQAASAKG
SGYPVADVAAAFLAVGVICQ
>Mature_500_residues
MKVYLLPSAVAVCIAASLQAQAVEIKVNHGQSLSAAAGLSQQHSFKAIKTVTFNKGQTKTKALQYFNGVLVYGHHLVVSN
DDSEQSAIGDVAQIADDFSVEAGISQGQAIAALNRLYPGSAATGKKDVELVILMQDDTPRLVYRVSYLSDQTDALSRPTG
FVDAQTGEVLEHWNEIMTAKNNVGKPGSGGGGSGGSSNPFDATGPGGNAKIGDYYYGSDYGFLKVSESNGTCTMTNANVK
TVNMANSTRRGSVFTFTCPENTYQTVNGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQLEMRVHYGRNYENAFWDGSAM
SFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLNYSGQSGGINEAFSDIAGEAAEFYMRGSNDWKVGYDIVKGNGALRYM
DNPTKDGRSIDNAANYTSSMDVHYSSGVFNKAFYTLATKNGWDTRKAFDVFVLANQLYWTPTSTFNKAACGVQQAASAKG
SGYPVADVAAAFLAVGVICQ

Specific function: Cleaves elastin, collagen, IgG, and several complement components. Involved in the pathogenesis of P.aeruginosa infections [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]

EC number: =3.4.24.26 [H]

Molecular weight: Translated: 53637; Mature: 53637

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYLLPSAVAVCIAASLQAQAVEIKVNHGQSLSAAAGLSQQHSFKAIKTVTFNKGQTKT
CEEEEEHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHCCCCCCCCEEEEEEEECCCCCHH
KALQYFNGVLVYGHHLVVSNDDSEQSAIGDVAQIADDFSVEAGISQGQAIAALNRLYPGS
HHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCC
AATGKKDVELVILMQDDTPRLVYRVSYLSDQTDALSRPTGFVDAQTGEVLEHWNEIMTAK
CCCCCCCEEEEEEEECCCCEEEEEEEHHCCCHHHHCCCCCCEECCCHHHHHHHHHHHHHC
NNVGKPGSGGGGSGGSSNPFDATGPGGNAKIGDYYYGSDYGFLKVSESNGTCTMTNANVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEECCCCEEEEEECCCCEEEEECCCEE
TVNMANSTRRGSVFTFTCPENTYQTVNGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQL
EEEECCCCCCCCEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
EMRVHYGRNYENAFWDGSAMSFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLNYSGQSG
EEEEEECCCCCCCEECCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCCCCCCC
GINEAFSDIAGEAAEFYMRGSNDWKVGYDIVKGNGALRYMDNPTKDGRSIDNAANYTSSM
CHHHHHHHHHCCHHHHEEECCCCCEEEEEEEECCCEEEECCCCCCCCCCCCHHHCCCCCC
DVHYSSGVFNKAFYTLATKNGWDTRKAFDVFVLANQLYWTPTSTFNKAACGVQQAASAKG
EEEECCCCCCCEEEEEEECCCCCCHHHEEEEEEECEEEECCCCCCCHHHHCHHHHHCCCC
SGYPVADVAAAFLAVGVICQ
CCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVYLLPSAVAVCIAASLQAQAVEIKVNHGQSLSAAAGLSQQHSFKAIKTVTFNKGQTKT
CEEEEEHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHCCCCCCCCEEEEEEEECCCCCHH
KALQYFNGVLVYGHHLVVSNDDSEQSAIGDVAQIADDFSVEAGISQGQAIAALNRLYPGS
HHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCC
AATGKKDVELVILMQDDTPRLVYRVSYLSDQTDALSRPTGFVDAQTGEVLEHWNEIMTAK
CCCCCCCEEEEEEEECCCCEEEEEEEHHCCCHHHHCCCCCCEECCCHHHHHHHHHHHHHC
NNVGKPGSGGGGSGGSSNPFDATGPGGNAKIGDYYYGSDYGFLKVSESNGTCTMTNANVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEECCCCEEEEEECCCCEEEEECCCEE
TVNMANSTRRGSVFTFTCPENTYQTVNGAYSPLNDAHYFGGVIFNMYKDWYNTAPLTFQL
EEEECCCCCCCCEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
EMRVHYGRNYENAFWDGSAMSFGDGASYFYPLVSLDVSAHEVSHGFTEQNSGLNYSGQSG
EEEEEECCCCCCCEECCCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCCCCCCCCCC
GINEAFSDIAGEAAEFYMRGSNDWKVGYDIVKGNGALRYMDNPTKDGRSIDNAANYTSSM
CHHHHHHHHHCCHHHHEEECCCCCEEEEEEEECCCEEEECCCCCCCCCCCCHHHCCCCCC
DVHYSSGVFNKAFYTLATKNGWDTRKAFDVFVLANQLYWTPTSTFNKAACGVQQAASAKG
EEEECCCCCCCEEEEEEECCCCCCHHHEEEEEEECEEEECCCCCCCHHHHCHHHHHCCCC
SGYPVADVAAAFLAVGVICQ
CCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2842313; 2493453; 1917848; 10984043; 1544509; 3034864; 2123831; 1899664 [H]