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The map label for this gene is polB [H]

Identifier: 117921070

GI number: 117921070

Start: 3136302

End: 3138734

Strand: Reverse

Name: polB [H]

Synonym: Shewana3_2629

Alternate gene names: 117921070

Gene position: 3138734-3136302 (Counterclockwise)

Preceding gene: 117921077

Following gene: 117921069

Centisome position: 63.13

GC content: 48.54

Gene sequence:

>2433_bases
TTGAATCCCCCCGAGAGTCTGACGCATGACACGGCTGCGCCTGTGAGTGTACAGGGACGCGTCCTCACGCGCCATGCTAT
TACACGCGGTGCAACGCTTGTGTTGCAGTATTATCTCGCCACCGTTTCAGGCCCTGTGCTGGTGGAGTTACCAGACTCTG
AGTACATTTGTTTTTGTCATCAAAGCGATATGGCGGCATTGCAGTTACAAACGCCGGGGCTGGCGCTGCGCTTTGTGCCG
CTTACACTTAAAAGCTTTAAAAGACAAAGCGTGGCGGCGATTTATGCTCCATCGAGTAGTGTGTTTCGTCACTTACAGCG
GATAGCGACAGATGCGGGGATCCCCTTATTTGAGGCGGATATCCGTCCCGAGCAGCGCTTTTTAATCGAGCGCTTTGTGG
CGCTCGATGTGGCATTTTTAGGGCATTTTGTCGGCAAAGCAGGCATAGATGGACAGTTGTCTGTTTTTAGCGCCATCCGC
GCCAAAGCGGTTGCGCCGCAGTCTGCAATTAAACTGCGCTCTATCTCCCTCGATTTTGAATGCAGTTTTGACGGTTTACT
CTATTCCGTGGCCCTTTATGGACGCGATGCGCAATCTCAGCCCTATGAAAAAGTCATTATGGTGGGCGAGGCGCAGCCAG
ATGCTGCAATCTATATCGAATGGGTGAACGATGAGGCCGCACTTATCCATCGTCTCATCACCTGGTTTACAGAGTTTGAT
CCCGATGTGATCATTGGTTGGTCGGTGGTGACCTTTGACTTGGCCCTGCTATATCGACGTGCGTTACTGCACCGGATCCC
GCTGCGTATTGGTCGAGGTGGCGCGCTCCTTGAGTGGAAAGTTGAGAATAAATTTCGGCCAGAAACCTTAAGTTTGCCAG
GGCGTGTGGTGCTCGATGGTATCGATTGGTTAAAGGCGGCCTTTTATCAATTCGAGCGTTTCTCCTTGGAGTTTGTCGCT
CAAGCGTTGCTGGGCGAAGGCAAAGCGATTCATGATGTAGAAAATCGAGCGCAAGAGATTGATTCGTTATTTGCCGAAAA
TAAACAAGGGCTTGCTCACTATAACCTGACTGATAGTCGCTTAGTGTGGGATATTTTTGAACATACGCAATTGTGGGATT
TTGCCCTCGCTCGCGCCGAGTTAACGGGGTTAGAACTGGGGCGCGTAGGCGCCTCGGTCGCCGCATTCAACCACTTGTAT
CTACCGCATTTACATAGAGCGGGTTTCGTCGCGCCCGCTGAGCCTGCCAGTCAAGGGATTGAAAGCCCAGGCGGTTATGT
TATGGATTCTGTCCCTGGTTTTTATCAGCATATTTTAGTATTTGACTTTAAAAGCCTTTATCCATCGATTATCCGAACTT
TTTTGATTGATCCTAAGGGGTTGATTGAAGGTTTAGATAATGAGGACGGATCTGCATTAGATGAGCCTGAAACTGTTCCG
GGTTTTTTGGGCGCGCGTTTTAATCGGCATCAACCTATTCTGCCCAAACTCATCCAAAATCTCTCAGAGCAACGTGAGAA
AGCTAAGCGGGAGGCGAATGCGCCTTTATCGCAAGCGATTAAAATCATTATGAATTCGCTATATGGGGTTTTAGGCTCCC
AAGGCTGTGTGTTCCACGATGCGAAACTGGCAAGCTCCATCACCATGCGCGGTCATCAAATTATGAAACAAACCCGCGCG
TGGATTGAGGAGATGGGCTACCAAGTCATTTATGGGGATACGGACTCCACCTTCGTCTATTTAGGCCCAGAACCCGATCT
CAGTGATATTAATGCCTTAGGTAAGCAGATTGCCGCGCGAATGAATCAGCAATGGCAAAGTAAAATTGCGCAGGAGTTTC
AGCTAGAAAGTTTTCTCGAGCTGCAATTTGAGCGCCACTACGAACAGTTTTTTATGCCGACCCTGCGGGGCTCGGAGGAG
GGGAGTAAGAAGCGCTATGTTGGAGCATGGCGTAACAATAGCGGAGCGTTAGAAATCACTTTTAAGGGAATGGAGCAGGT
TCGTAGCGATTGGAGCCCCTTAGCCCGCAAGGTGCAGGCTGAACTCTATGAGCGCATGTTTAACCAACGGGATATTAGTG
GTTATTTGGCTGATGTTATCGGCGAACTTCAAGCGGGAAAGCGCGATGATGAGTTAGTGTTTAGTAAACGTATGCGCCGT
AATTTAGATGAATACACGGCTAAGTCTTCTCCCCATGTGAAAGTCGCGCGCCAGCTTTGTGAGTTAACAGGAAAGTCATC
CTTTGGCAAGCGAGGGGCGCAGATTGATTATGTAATTACTGTAAACGGTCCTGAACCCGTTTCGCATAGATCGTCTACAA
TTGATTATCAGTACTACATTGATAAGCAAATTGGCCCTATTGCAGAGCCTGTTTTTTCAATAATGAAACTAAATTACACT
AGCATTACGTCGAATCAGCTGTTGTTAATTTAG

Upstream 100 bases:

>100_bases
GTGATTGCATAGCCTAAGAAACTTTTCTGCCATTTTATGGCAATTTTATTCTGTGTCCCGAGAATCAATAAACAGCGAGA
GCCTTAGAGTGAAACCGCAG

Downstream 100 bases:

>100_bases
TGTTTCCATTGTGTGTTTTGTGTTTTGTTTGCTGGTCTTTGTAGTGTTTTAACCTGTTTTATAGAGGTTAAACACGCTGT
TTTTGTGATGTTTCTCTACA

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MNPPESLTHDTAAPVSVQGRVLTRHAITRGATLVLQYYLATVSGPVLVELPDSEYICFCHQSDMAALQLQTPGLALRFVP
LTLKSFKRQSVAAIYAPSSSVFRHLQRIATDAGIPLFEADIRPEQRFLIERFVALDVAFLGHFVGKAGIDGQLSVFSAIR
AKAVAPQSAIKLRSISLDFECSFDGLLYSVALYGRDAQSQPYEKVIMVGEAQPDAAIYIEWVNDEAALIHRLITWFTEFD
PDVIIGWSVVTFDLALLYRRALLHRIPLRIGRGGALLEWKVENKFRPETLSLPGRVVLDGIDWLKAAFYQFERFSLEFVA
QALLGEGKAIHDVENRAQEIDSLFAENKQGLAHYNLTDSRLVWDIFEHTQLWDFALARAELTGLELGRVGASVAAFNHLY
LPHLHRAGFVAPAEPASQGIESPGGYVMDSVPGFYQHILVFDFKSLYPSIIRTFLIDPKGLIEGLDNEDGSALDEPETVP
GFLGARFNRHQPILPKLIQNLSEQREKAKREANAPLSQAIKIIMNSLYGVLGSQGCVFHDAKLASSITMRGHQIMKQTRA
WIEEMGYQVIYGDTDSTFVYLGPEPDLSDINALGKQIAARMNQQWQSKIAQEFQLESFLELQFERHYEQFFMPTLRGSEE
GSKKRYVGAWRNNSGALEITFKGMEQVRSDWSPLARKVQAELYERMFNQRDISGYLADVIGELQAGKRDDELVFSKRMRR
NLDEYTAKSSPHVKVARQLCELTGKSSFGKRGAQIDYVITVNGPEPVSHRSSTIDYQYYIDKQIGPIAEPVFSIMKLNYT
SITSNQLLLI

Sequences:

>Translated_810_residues
MNPPESLTHDTAAPVSVQGRVLTRHAITRGATLVLQYYLATVSGPVLVELPDSEYICFCHQSDMAALQLQTPGLALRFVP
LTLKSFKRQSVAAIYAPSSSVFRHLQRIATDAGIPLFEADIRPEQRFLIERFVALDVAFLGHFVGKAGIDGQLSVFSAIR
AKAVAPQSAIKLRSISLDFECSFDGLLYSVALYGRDAQSQPYEKVIMVGEAQPDAAIYIEWVNDEAALIHRLITWFTEFD
PDVIIGWSVVTFDLALLYRRALLHRIPLRIGRGGALLEWKVENKFRPETLSLPGRVVLDGIDWLKAAFYQFERFSLEFVA
QALLGEGKAIHDVENRAQEIDSLFAENKQGLAHYNLTDSRLVWDIFEHTQLWDFALARAELTGLELGRVGASVAAFNHLY
LPHLHRAGFVAPAEPASQGIESPGGYVMDSVPGFYQHILVFDFKSLYPSIIRTFLIDPKGLIEGLDNEDGSALDEPETVP
GFLGARFNRHQPILPKLIQNLSEQREKAKREANAPLSQAIKIIMNSLYGVLGSQGCVFHDAKLASSITMRGHQIMKQTRA
WIEEMGYQVIYGDTDSTFVYLGPEPDLSDINALGKQIAARMNQQWQSKIAQEFQLESFLELQFERHYEQFFMPTLRGSEE
GSKKRYVGAWRNNSGALEITFKGMEQVRSDWSPLARKVQAELYERMFNQRDISGYLADVIGELQAGKRDDELVFSKRMRR
NLDEYTAKSSPHVKVARQLCELTGKSSFGKRGAQIDYVITVNGPEPVSHRSSTIDYQYYIDKQIGPIAEPVFSIMKLNYT
SITSNQLLLI
>Mature_810_residues
MNPPESLTHDTAAPVSVQGRVLTRHAITRGATLVLQYYLATVSGPVLVELPDSEYICFCHQSDMAALQLQTPGLALRFVP
LTLKSFKRQSVAAIYAPSSSVFRHLQRIATDAGIPLFEADIRPEQRFLIERFVALDVAFLGHFVGKAGIDGQLSVFSAIR
AKAVAPQSAIKLRSISLDFECSFDGLLYSVALYGRDAQSQPYEKVIMVGEAQPDAAIYIEWVNDEAALIHRLITWFTEFD
PDVIIGWSVVTFDLALLYRRALLHRIPLRIGRGGALLEWKVENKFRPETLSLPGRVVLDGIDWLKAAFYQFERFSLEFVA
QALLGEGKAIHDVENRAQEIDSLFAENKQGLAHYNLTDSRLVWDIFEHTQLWDFALARAELTGLELGRVGASVAAFNHLY
LPHLHRAGFVAPAEPASQGIESPGGYVMDSVPGFYQHILVFDFKSLYPSIIRTFLIDPKGLIEGLDNEDGSALDEPETVP
GFLGARFNRHQPILPKLIQNLSEQREKAKREANAPLSQAIKIIMNSLYGVLGSQGCVFHDAKLASSITMRGHQIMKQTRA
WIEEMGYQVIYGDTDSTFVYLGPEPDLSDINALGKQIAARMNQQWQSKIAQEFQLESFLELQFERHYEQFFMPTLRGSEE
GSKKRYVGAWRNNSGALEITFKGMEQVRSDWSPLARKVQAELYERMFNQRDISGYLADVIGELQAGKRDDELVFSKRMRR
NLDEYTAKSSPHVKVARQLCELTGKSSFGKRGAQIDYVITVNGPEPVSHRSSTIDYQYYIDKQIGPIAEPVFSIMKLNYT
SITSNQLLLI

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=703, Percent_Identity=26.0312944523471, Blast_Score=154, Evalue=4e-37,
Organism=Homo sapiens, GI106507301, Length=659, Percent_Identity=23.2169954476479, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI1786246, Length=803, Percent_Identity=42.4657534246575, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17559488, Length=699, Percent_Identity=23.6051502145923, Blast_Score=136, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32565317, Length=627, Percent_Identity=24.4019138755981, Blast_Score=126, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320101, Length=699, Percent_Identity=23.8912732474964, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324227, Length=645, Percent_Identity=22.4806201550388, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6325090, Length=436, Percent_Identity=26.1467889908257, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24664937, Length=747, Percent_Identity=25.1673360107095, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24648780, Length=653, Percent_Identity=22.052067381317, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 91027; Mature: 91027

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPESLTHDTAAPVSVQGRVLTRHAITRGATLVLQYYLATVSGPVLVELPDSEYICFCH
CCCCCCCCCCCCCCCEECCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEE
QSDMAALQLQTPGLALRFVPLTLKSFKRQSVAAIYAPSSSVFRHLQRIATDAGIPLFEAD
CCCCEEEEEECCCEEEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECC
IRPEQRFLIERFVALDVAFLGHFVGKAGIDGQLSVFSAIRAKAVAPQSAIKLRSISLDFE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHEEEEEEEEEE
CSFDGLLYSVALYGRDAQSQPYEKVIMVGEAQPDAAIYIEWVNDEAALIHRLITWFTEFD
CCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
PDVIIGWSVVTFDLALLYRRALLHRIPLRIGRGGALLEWKVENKFRPETLSLPGRVVLDG
CCEEEEHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEEEECCCCCCCCCCCCCEEEECH
IDWLKAAFYQFERFSLEFVAQALLGEGKAIHDVENRAQEIDSLFAENKQGLAHYNLTDSR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCH
LVWDIFEHTQLWDFALARAELTGLELGRVGASVAAFNHLYLPHLHRAGFVAPAEPASQGI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
ESPGGYVMDSVPGFYQHILVFDFKSLYPSIIRTFLIDPKGLIEGLDNEDGSALDEPETVP
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCC
GFLGARFNRHQPILPKLIQNLSEQREKAKREANAPLSQAIKIIMNSLYGVLGSQGCVFHD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEH
AKLASSITMRGHQIMKQTRAWIEEMGYQVIYGDTDSTFVYLGPEPDLSDINALGKQIAAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHH
MNQQWQSKIAQEFQLESFLELQFERHYEQFFMPTLRGSEEGSKKRYVGAWRNNSGALEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCEEEEEECCCCEEEEE
FKGMEQVRSDWSPLARKVQAELYERMFNQRDISGYLADVIGELQAGKRDDELVFSKRMRR
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
NLDEYTAKSSPHVKVARQLCELTGKSSFGKRGAQIDYVITVNGPEPVSHRSSTIDYQYYI
CHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEE
DKQIGPIAEPVFSIMKLNYTSITSNQLLLI
CCCCCHHHHHHHHHHHCCCEEECCCCEEEC
>Mature Secondary Structure
MNPPESLTHDTAAPVSVQGRVLTRHAITRGATLVLQYYLATVSGPVLVELPDSEYICFCH
CCCCCCCCCCCCCCCEECCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEE
QSDMAALQLQTPGLALRFVPLTLKSFKRQSVAAIYAPSSSVFRHLQRIATDAGIPLFEAD
CCCCEEEEEECCCEEEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECC
IRPEQRFLIERFVALDVAFLGHFVGKAGIDGQLSVFSAIRAKAVAPQSAIKLRSISLDFE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHEEEEEEEEEE
CSFDGLLYSVALYGRDAQSQPYEKVIMVGEAQPDAAIYIEWVNDEAALIHRLITWFTEFD
CCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
PDVIIGWSVVTFDLALLYRRALLHRIPLRIGRGGALLEWKVENKFRPETLSLPGRVVLDG
CCEEEEHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEEEECCCCCCCCCCCCCEEEECH
IDWLKAAFYQFERFSLEFVAQALLGEGKAIHDVENRAQEIDSLFAENKQGLAHYNLTDSR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCH
LVWDIFEHTQLWDFALARAELTGLELGRVGASVAAFNHLYLPHLHRAGFVAPAEPASQGI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCC
ESPGGYVMDSVPGFYQHILVFDFKSLYPSIIRTFLIDPKGLIEGLDNEDGSALDEPETVP
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCC
GFLGARFNRHQPILPKLIQNLSEQREKAKREANAPLSQAIKIIMNSLYGVLGSQGCVFHD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEH
AKLASSITMRGHQIMKQTRAWIEEMGYQVIYGDTDSTFVYLGPEPDLSDINALGKQIAAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHH
MNQQWQSKIAQEFQLESFLELQFERHYEQFFMPTLRGSEEGSKKRYVGAWRNNSGALEIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCEEEEEECCCCEEEEE
FKGMEQVRSDWSPLARKVQAELYERMFNQRDISGYLADVIGELQAGKRDDELVFSKRMRR
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
NLDEYTAKSSPHVKVARQLCELTGKSSFGKRGAQIDYVITVNGPEPVSHRSSTIDYQYYI
CHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEE
DKQIGPIAEPVFSIMKLNYTSITSNQLLLI
CCCCCHHHHHHHHHHHCCCEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]