Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 117921055

GI number: 117921055

Start: 3120963

End: 3121925

Strand: Direct

Name: gcvA [H]

Synonym: Shewana3_2614

Alternate gene names: 117921055

Gene position: 3120963-3121925 (Clockwise)

Preceding gene: 117921050

Following gene: 117921061

Centisome position: 62.77

GC content: 49.84

Gene sequence:

>963_bases
GTGAGCAAAGCTCACACTAAATGCCCTTATCATTTACTCGATTCGGATACGCCTATGTTTACTTCCTTGCCGCCCCTCAA
TGCATTACGGGCTTTCGAGTCTGCCGCCCGCTTGCAAAGCATGACTCTTGCCAGCAAAGAACTGCATGTCACCCACGGCG
CCATCAGTAAACAAATCCGGGTATTAGAAGAATTCTTAGGTTTTGCCCTCTTCGTGCGTCTGCATAAAAAAGTCGCACTC
ACGAACGAAGCCAAGCGCTACTTGCCCCATGTGCAAGCGGCTCTGCAAACTTTGTCCAGTGCCACCGCCGATTTACGTAG
CCAAGGCTCGCGGCCACAAACTTTAGCGATCAATGTGTTACCCAGTCTAACCATCAACTGGTTAATCCCCCGGATGGAGC
AATTTAAATCTCGGCATCCGCATTTGTATGTGGATCTCTCGATTGGTGACTTTGCCGTCGATTTCAGCCAAGGCCGTTAT
GACATTGCCATTCGCAGCAGCACTCAACTCCCCAGAGGTGTTAATTACATCAAACTGATGGATGAAGACTTGTGCTTAGT
GTGCGCGCCGTCACTCGCGCCTAAGCTCAAAAGCATCGAAGACATCAATCAAGTTACGCTGCTGAAACACACCACACGGC
CAGAGCTTTGGGAATATTGGGCGGATAAAGTGGGACTGGTACTCACGCAGGCACAAACCTTTGGCGTCGAGCATTTTTAT
ATGTTGAGTCAAGCCGCAGCCAGTGGCATGGGCGCAGCGCTGATCCCACGTTTTTTTATCGAAGAACAATTGGCCAATGG
CAGCTTGGTGATCCCGTTTGAAGCGCCTTTTACCAGCCCATACGCCTATTACTTACTCACGCCCAAATCCCCTAGCCTAC
CGCTAAAAGTACAAGCCTTTATCGATTGGATGCTCGAGTTATTTTCCCCATATCGGCAATCGTCAGATAACGACAGAGCC
TAG

Upstream 100 bases:

>100_bases
GTTCAAATTCCATGATTGTTATTATTGAGTAATAGGAGCTAAGGCTTCGCATTAGACTGCGTTTGCCTTATAAACGCAAA
GGAGTTATTGTCGCGCTAGC

Downstream 100 bases:

>100_bases
AGAGTAAAAAACCACCTGAGCGGTGGTTTTTTAACGGCTTATCCGGAGATAGGCTTAGTGATGGCAATGTTCATCGTGAA
CATGGTCGTCATCCATAAAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSKAHTKCPYHLLDSDTPMFTSLPPLNALRAFESAARLQSMTLASKELHVTHGAISKQIRVLEEFLGFALFVRLHKKVAL
TNEAKRYLPHVQAALQTLSSATADLRSQGSRPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRY
DIAIRSSTQLPRGVNYIKLMDEDLCLVCAPSLAPKLKSIEDINQVTLLKHTTRPELWEYWADKVGLVLTQAQTFGVEHFY
MLSQAAASGMGAALIPRFFIEEQLANGSLVIPFEAPFTSPYAYYLLTPKSPSLPLKVQAFIDWMLELFSPYRQSSDNDRA

Sequences:

>Translated_320_residues
MSKAHTKCPYHLLDSDTPMFTSLPPLNALRAFESAARLQSMTLASKELHVTHGAISKQIRVLEEFLGFALFVRLHKKVAL
TNEAKRYLPHVQAALQTLSSATADLRSQGSRPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRY
DIAIRSSTQLPRGVNYIKLMDEDLCLVCAPSLAPKLKSIEDINQVTLLKHTTRPELWEYWADKVGLVLTQAQTFGVEHFY
MLSQAAASGMGAALIPRFFIEEQLANGSLVIPFEAPFTSPYAYYLLTPKSPSLPLKVQAFIDWMLELFSPYRQSSDNDRA
>Mature_319_residues
SKAHTKCPYHLLDSDTPMFTSLPPLNALRAFESAARLQSMTLASKELHVTHGAISKQIRVLEEFLGFALFVRLHKKVALT
NEAKRYLPHVQAALQTLSSATADLRSQGSRPQTLAINVLPSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYD
IAIRSSTQLPRGVNYIKLMDEDLCLVCAPSLAPKLKSIEDINQVTLLKHTTRPELWEYWADKVGLVLTQAQTFGVEHFYM
LSQAAASGMGAALIPRFFIEEQLANGSLVIPFEAPFTSPYAYYLLTPKSPSLPLKVQAFIDWMLELFSPYRQSSDNDRA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=35.3951890034364, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=30.3886925795053, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=30, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=25.4180602006689, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=25.2491694352159, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=29.6442687747036, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=28.4883720930233, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788887, Length=165, Percent_Identity=28.4848484848485, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35967; Mature: 35836

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAHTKCPYHLLDSDTPMFTSLPPLNALRAFESAARLQSMTLASKELHVTHGAISKQIR
CCCCCCCCCEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHH
VLEEFLGFALFVRLHKKVALTNEAKRYLPHVQAALQTLSSATADLRSQGSRPQTLAINVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSSTQLPRGVNYIKLM
CCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCEEEEEEECCCCCCCCCCEEEEE
DEDLCLVCAPSLAPKLKSIEDINQVTLLKHTTRPELWEYWADKVGLVLTQAQTFGVEHFY
CCCEEEEECCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCEEEEHHHHHCHHHHH
MLSQAAASGMGAALIPRFFIEEQLANGSLVIPFEAPFTSPYAYYLLTPKSPSLPLKVQAF
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHH
IDWMLELFSPYRQSSDNDRA
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKAHTKCPYHLLDSDTPMFTSLPPLNALRAFESAARLQSMTLASKELHVTHGAISKQIR
CCCCCCCCEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHH
VLEEFLGFALFVRLHKKVALTNEAKRYLPHVQAALQTLSSATADLRSQGSRPQTLAINVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PSLTINWLIPRMEQFKSRHPHLYVDLSIGDFAVDFSQGRYDIAIRSSTQLPRGVNYIKLM
CCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCEEEEEEECCCCCCCCCCEEEEE
DEDLCLVCAPSLAPKLKSIEDINQVTLLKHTTRPELWEYWADKVGLVLTQAQTFGVEHFY
CCCEEEEECCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCEEEEHHHHHCHHHHH
MLSQAAASGMGAALIPRFFIEEQLANGSLVIPFEAPFTSPYAYYLLTPKSPSLPLKVQAF
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHH
IDWMLELFSPYRQSSDNDRA
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]