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The map label for this gene is dosC [H]

Identifier: 117920984

GI number: 117920984

Start: 3040145

End: 3041566

Strand: Reverse

Name: dosC [H]

Synonym: Shewana3_2543

Alternate gene names: 117920984

Gene position: 3041566-3040145 (Counterclockwise)

Preceding gene: 117920985

Following gene: 117920982

Centisome position: 61.17

GC content: 46.13

Gene sequence:

>1422_bases
ATGATCGCAGGGATTATGATTAGTACAAAACTCAGTTGGGCACTGGTTCGTCAATCCTGTTCACTGGCAACATTGATATT
TGTTGTCCTGCTGCTCATTCTCTTACCCTTCAAAATCGATAAGGAACATAAGGCTTTCGTTCATTTCCTGAGGGCAAAAC
ACACTGTGCCAAGTTATGTGGAGCTGCTTGGGAAAGGATTGTCGTTACACGATATTATTGATTTTGATAGCGCGCTGCTT
GCGCCCTTTAAGCTCGACCCTTCGCGCTTATTATTGCCTCCTGGAAGTCATTTACTGCCGGAAGAAGAGCAAATCATTCG
TTATTTTGACTACCTCTATTCGAGTCGCAAGAATGCGGTTCGCGGGGCTTTTGTCTATATCGGCTTATATAAAAGCAATG
CCTTGTATGTGGCATCCCCAATTCCTGAAGAAGTGAAAAACCGATTAATTCACGATCACATTTTGACCAATGAGTTTTGT
CAGCGGCTGCATTATTGTAGTAAGGACGCGTCGGCGGAGTCCTTGGCGGACGGTATCGTGTTATCGATTCCTTATGTTGA
TGAAATTACAGGAAATGTAATTTTCTCTATGGCGGCACCGATTTCATCTTCCCACGATCCTGCGCACCTGTGGGGGGATG
TGATTGCCGATACGGCATTTATGGCATCGGGATTTATGCGGGGTAAAGGCTATGAGTCGGTGATGACTCCTTATGGCACA
GATATAGTGATCACTGGCGACAGCCTCTGGACGCGTATTACGCCAGAGTTCTTGTTTTATCCCATGAGTAATCATCTGGA
TAACCTCACCACATTAACCTACAAAGTCAGTGTATTACGCTTTGTGGTTCAGTATGTTCCCTTATGGTTCTTGTTGTGCG
TGTTAACTGTTATCTTCCAACTCAGATATATGCGCCTTAAACAGCTGCGAGCAGAACGTCAACGGTTCGGCGAAGCGATG
CTGCAGGATCCTTTTACTAAAACCTACAATAAGAAGATCTATGAGACGCTGACATTTAAGGCGGCGACCGAGGGCCTGTA
TGCGGTGATCTGTATCGATGGCGATAAAATCAAAACCATCAATGACACCTACGGACATAAACTGGGCGATATGGCGATTC
TGCAAATTGTTGAAGCCATGCGGGCGGTATTTCGGGAGAATGACTTGTTTATCCGCACTGGTGGGGACGAATTTGTGGTG
ATCCTCAGTAATTGCTCCCAGGATAAAGCGTTACAACTTGCTCATAAGCTTAAAGAGAATGTTGAGCAGCGCCAGTTCTT
AAGCATGCTAACGGTATCGTGCGGGGTGGCAGATTCGAGCGGCTATCCATCCTTTGACTCAGTGTTACAGGCGGCGGATA
AGGCTTTATACGTGGATAAGGCCAAAAAGGGTGGGCATGTGTCCGATAATAGCCTGCGCTGA

Upstream 100 bases:

>100_bases
GCCCTTCCCACTGCTTGAACTAGAGTTAACAATAAGATTTATTTCAATTTAATACTTGAACAATACAAATGTTCGTTATA
TTCAATTCATTTTAAGTAAG

Downstream 100 bases:

>100_bases
GTTCCTTTTACTGAGCTAGCTAGACATTAAAAATTAGGCATAAAAAAGCGTGGTCTTGCCACGCTTTTTTATTGCTATCT
GCCCTAGAGCACCATGGCGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIAGIMISTKLSWALVRQSCSLATLIFVVLLLILLPFKIDKEHKAFVHFLRAKHTVPSYVELLGKGLSLHDIIDFDSALL
APFKLDPSRLLLPPGSHLLPEEEQIIRYFDYLYSSRKNAVRGAFVYIGLYKSNALYVASPIPEEVKNRLIHDHILTNEFC
QRLHYCSKDASAESLADGIVLSIPYVDEITGNVIFSMAAPISSSHDPAHLWGDVIADTAFMASGFMRGKGYESVMTPYGT
DIVITGDSLWTRITPEFLFYPMSNHLDNLTTLTYKVSVLRFVVQYVPLWFLLCVLTVIFQLRYMRLKQLRAERQRFGEAM
LQDPFTKTYNKKIYETLTFKAATEGLYAVICIDGDKIKTINDTYGHKLGDMAILQIVEAMRAVFRENDLFIRTGGDEFVV
ILSNCSQDKALQLAHKLKENVEQRQFLSMLTVSCGVADSSGYPSFDSVLQAADKALYVDKAKKGGHVSDNSLR

Sequences:

>Translated_473_residues
MIAGIMISTKLSWALVRQSCSLATLIFVVLLLILLPFKIDKEHKAFVHFLRAKHTVPSYVELLGKGLSLHDIIDFDSALL
APFKLDPSRLLLPPGSHLLPEEEQIIRYFDYLYSSRKNAVRGAFVYIGLYKSNALYVASPIPEEVKNRLIHDHILTNEFC
QRLHYCSKDASAESLADGIVLSIPYVDEITGNVIFSMAAPISSSHDPAHLWGDVIADTAFMASGFMRGKGYESVMTPYGT
DIVITGDSLWTRITPEFLFYPMSNHLDNLTTLTYKVSVLRFVVQYVPLWFLLCVLTVIFQLRYMRLKQLRAERQRFGEAM
LQDPFTKTYNKKIYETLTFKAATEGLYAVICIDGDKIKTINDTYGHKLGDMAILQIVEAMRAVFRENDLFIRTGGDEFVV
ILSNCSQDKALQLAHKLKENVEQRQFLSMLTVSCGVADSSGYPSFDSVLQAADKALYVDKAKKGGHVSDNSLR
>Mature_473_residues
MIAGIMISTKLSWALVRQSCSLATLIFVVLLLILLPFKIDKEHKAFVHFLRAKHTVPSYVELLGKGLSLHDIIDFDSALL
APFKLDPSRLLLPPGSHLLPEEEQIIRYFDYLYSSRKNAVRGAFVYIGLYKSNALYVASPIPEEVKNRLIHDHILTNEFC
QRLHYCSKDASAESLADGIVLSIPYVDEITGNVIFSMAAPISSSHDPAHLWGDVIADTAFMASGFMRGKGYESVMTPYGT
DIVITGDSLWTRITPEFLFYPMSNHLDNLTTLTYKVSVLRFVVQYVPLWFLLCVLTVIFQLRYMRLKQLRAERQRFGEAM
LQDPFTKTYNKKIYETLTFKAATEGLYAVICIDGDKIKTINDTYGHKLGDMAILQIVEAMRAVFRENDLFIRTGGDEFVV
ILSNCSQDKALQLAHKLKENVEQRQFLSMLTVSCGVADSSGYPSFDSVLQAADKALYVDKAKKGGHVSDNSLR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=191, Percent_Identity=31.413612565445, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=32.2404371584699, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=240, Percent_Identity=28.3333333333333, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787262, Length=156, Percent_Identity=32.0512820512821, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=192, Percent_Identity=30.2083333333333, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=26.1904761904762, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082007, Length=137, Percent_Identity=30.6569343065693, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788381, Length=148, Percent_Identity=27.027027027027, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787056, Length=145, Percent_Identity=33.7931034482759, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=29.6969696969697, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 53286; Mature: 53286

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGIMISTKLSWALVRQSCSLATLIFVVLLLILLPFKIDKEHKAFVHFLRAKHTVPSYV
CCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
ELLGKGLSLHDIIDFDSALLAPFKLDPSRLLLPPGSHLLPEEEQIIRYFDYLYSSRKNAV
HHHHCCCCHHHHHCCCHHHCCCEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCC
RGAFVYIGLYKSNALYVASPIPEEVKNRLIHDHILTNEFCQRLHYCSKDASAESLADGIV
CEEEEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEE
LSIPYVDEITGNVIFSMAAPISSSHDPAHLWGDVIADTAFMASGFMRGKGYESVMTPYGT
EECCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
DIVITGDSLWTRITPEFLFYPMSNHLDNLTTLTYKVSVLRFVVQYVPLWFLLCVLTVIFQ
EEEEECCCHHHHCCHHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRYMRLKQLRAERQRFGEAMLQDPFTKTYNKKIYETLTFKAATEGLYAVICIDGDKIKTI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEC
NDTYGHKLGDMAILQIVEAMRAVFRENDLFIRTGGDEFVVILSNCSQDKALQLAHKLKEN
CHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHH
VEQRQFLSMLTVSCGVADSSGYPSFDSVLQAADKALYVDKAKKGGHVSDNSLR
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MIAGIMISTKLSWALVRQSCSLATLIFVVLLLILLPFKIDKEHKAFVHFLRAKHTVPSYV
CCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
ELLGKGLSLHDIIDFDSALLAPFKLDPSRLLLPPGSHLLPEEEQIIRYFDYLYSSRKNAV
HHHHCCCCHHHHHCCCHHHCCCEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCC
RGAFVYIGLYKSNALYVASPIPEEVKNRLIHDHILTNEFCQRLHYCSKDASAESLADGIV
CEEEEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEE
LSIPYVDEITGNVIFSMAAPISSSHDPAHLWGDVIADTAFMASGFMRGKGYESVMTPYGT
EECCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
DIVITGDSLWTRITPEFLFYPMSNHLDNLTTLTYKVSVLRFVVQYVPLWFLLCVLTVIFQ
EEEEECCCHHHHCCHHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRYMRLKQLRAERQRFGEAMLQDPFTKTYNKKIYETLTFKAATEGLYAVICIDGDKIKTI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEC
NDTYGHKLGDMAILQIVEAMRAVFRENDLFIRTGGDEFVVILSNCSQDKALQLAHKLKEN
CHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHH
VEQRQFLSMLTVSCGVADSSGYPSFDSVLQAADKALYVDKAKKGGHVSDNSLR
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]