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The map label for this gene is rnt [H]

Identifier: 117920981

GI number: 117920981

Start: 3037023

End: 3037691

Strand: Direct

Name: rnt [H]

Synonym: Shewana3_2540

Alternate gene names: 117920981

Gene position: 3037023-3037691 (Clockwise)

Preceding gene: 117920979

Following gene: 117920983

Centisome position: 61.08

GC content: 49.78

Gene sequence:

>669_bases
ATGAGCGATATTAGCGACGCAAACAAATTGAAACACCGATTTCGAGGCTACTTTCCGGTAGTGATAGATGTCGAAACCGC
GGGCTTCAACTCTCAAACCGATGCGTTGCTGGAAATAGCCGTCACGCTGTTAAAAATGGATGATGAAGGCTTGCTCGGCA
TTGATAAAACCCTGCATTTCAATATTGAGCCCTTTGAAGGCGCCAACTTAGAACCTGAGGCGCTGGCCTTTAATGGTATT
GATCCCACCAATCCATTACGCGGCGCCGTAAGCGAAAAAGAAGCCTTCTTAGAGATTTTTAAAGCCGTTAAAAAGGCGCA
GAAGGCCTCAGATTGTCACCGCAGTATTATCGTGGCGCACAATGCCGCCTTCGATCATGGCTTTGTGAGTAAAGCGATTG
AGCGCTGTGACTTAAAACGATCCCCCTTCCACCCTTTTGCCACCTTTGATACGGCAACACTCGCAGGCTTAGCGATTGGC
CATACCGTGCTGGCCAAAGCCTGCATCATGGCGGGGATCCCTTTCGATAATAAAGAGGCTCACTCAGCACTTTACGATAC
CGAGCGCACCGCAGAGCTGTTTTGCCATATCGTCAACCGCTGGAAGCAGTTAGGTGGTTGGCCATTATTAGCCGCGGGTG
AGTCAGAGGATGCAGATGGTGAGGAATAG

Upstream 100 bases:

>100_bases
AACTATTTAGTTCCTTTAGCTCATAAAACCACCAATCAAACATGACACATCCTAGTACATTTAGCATAATAGCGCCCTAT
CATTTTTCTTGGGTACTTTA

Downstream 100 bases:

>100_bases
GCGTTAATGCTTATCGACCGTCCAATAAAAAAGCTGCCCTAGGGCAGCTTTATTATTACGCGAAAATCCGCAAATTACAG
AGAGCTTGCGTTTTCTGCTA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSDISDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGLLGIDKTLHFNIEPFEGANLEPEALAFNGI
DPTNPLRGAVSEKEAFLEIFKAVKKAQKASDCHRSIIVAHNAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIG
HTVLAKACIMAGIPFDNKEAHSALYDTERTAELFCHIVNRWKQLGGWPLLAAGESEDADGEE

Sequences:

>Translated_222_residues
MSDISDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGLLGIDKTLHFNIEPFEGANLEPEALAFNGI
DPTNPLRGAVSEKEAFLEIFKAVKKAQKASDCHRSIIVAHNAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIG
HTVLAKACIMAGIPFDNKEAHSALYDTERTAELFCHIVNRWKQLGGWPLLAAGESEDADGEE
>Mature_221_residues
SDISDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGLLGIDKTLHFNIEPFEGANLEPEALAFNGID
PTNPLRGAVSEKEAFLEIFKAVKKAQKASDCHRSIIVAHNAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIGH
TVLAKACIMAGIPFDNKEAHSALYDTERTAELFCHIVNRWKQLGGWPLLAAGESEDADGEE

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=214, Percent_Identity=70.0934579439252, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24312; Mature: 24181

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDISDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGLLGIDKTLHF
CCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECCCEEEE
NIEPFEGANLEPEALAFNGIDPTNPLRGAVSEKEAFLEIFKAVKKAQKASDCHRSIIVAH
ECCCCCCCCCCHHHEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
NAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIGHTVLAKACIMAGIPFDNKEA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTERTAELFCHIVNRWKQLGGWPLLAAGESEDADGEE
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDISDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGLLGIDKTLHF
CCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECCCEEEE
NIEPFEGANLEPEALAFNGIDPTNPLRGAVSEKEAFLEIFKAVKKAQKASDCHRSIIVAH
ECCCCCCCCCCHHHEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
NAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIGHTVLAKACIMAGIPFDNKEA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTERTAELFCHIVNRWKQLGGWPLLAAGESEDADGEE
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA