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The map label for this gene is nadE [H]

Identifier: 117920881

GI number: 117920881

Start: 2923981

End: 2924871

Strand: Reverse

Name: nadE [H]

Synonym: Shewana3_2438

Alternate gene names: 117920881

Gene position: 2924871-2923981 (Counterclockwise)

Preceding gene: 117920882

Following gene: 117920879

Centisome position: 58.82

GC content: 48.37

Gene sequence:

>891_bases
ATGATAACGTTAGCCCAATCACTGCTCCCAATAACAGCGAATATTAAGGTGGAATTTTCGGTGAAAGCGCAGATCTTAAC
AGAAATGAAGGTACTCAAGACCATCGATCCTGAGTTCGAAGTGCAGCGCCGTGTGGCGTTTATTAAATCTAAACTGCAAG
CTGCCCGCAGCAAAGCCTTGGTCCTTGGGATCAGCGGTGGTGTGGATTCTTCCACGGCGGGACGTTTATGTCAGTTGGCC
GTTGATAGCCTCAATAGCGAGCATACTGACGGCGGTTATCAGTTTATTGCGGTGCGCTTACCCTATCAAATTCAAAAAGA
TGAGCACGAAGCTCAGCAAGCTTGCCAATTTATTCAGCCGACCAAATTAGTCACCATCAATGTGCATCAAGGGGTCGATG
GTGTGCATCAGGCGACTTTAACGGCCTTTGTCGAAGCGGGATTGCATTCACCGGATGCGGCTAAAGTTGATTTTATTAAA
GGTAACGTGAAAGCGCGCATGCGCATGATTGCCCAGTATGAGTTAGCCGGTTTAGTGGGCGGGCTTGTAGTGGGAACCGA
TCATAGCGCCGAGAATATCACCGGTTTTTATACCAAATGGGGCGATGGCGCCTGCGATCTTGCGCCTTTATTTGGACTTA
ACAAACGCCAAGTACGCCAGGTGGCAGCATATTTGGGCGCGCCAGAGTCCTTAGTCTATAAAGCGCCAACGGCGGATTTG
GAAGACAATCAACCACTACTGGAAGATGAAGTCGCGCTAGGGCTGACCTACGAGCAAATCGATGATTTCCTTGAAGGGAA
AGAAGTCGGCAAGGATGTTGAAGAAAAGCTTATCAATATCTACAAGGCCACGCAGCATAAGCGTCAACCTATCCCCACTA
TTTACGATTAA

Upstream 100 bases:

>100_bases
CCTTGTTGTTTTTTGGAACAGACGAAAACTTGAGCCAATGACGTTTTCTCAACAATTAACCCTGTGGATGGTAATTTTTG
CTACAGGCAGACCCGAATAA

Downstream 100 bases:

>100_bases
TTTAAGACGGTTACCGTCTGAGACATAACGAAAAAGCACCCTAGTGGGTGCTTTTTCATGTGTGAATGCTTAATGCTATC
TCGCCAAGTGTCTCGATTCG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MITLAQSLLPITANIKVEFSVKAQILTEMKVLKTIDPEFEVQRRVAFIKSKLQAARSKALVLGISGGVDSSTAGRLCQLA
VDSLNSEHTDGGYQFIAVRLPYQIQKDEHEAQQACQFIQPTKLVTINVHQGVDGVHQATLTAFVEAGLHSPDAAKVDFIK
GNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQVAAYLGAPESLVYKAPTADL
EDNQPLLEDEVALGLTYEQIDDFLEGKEVGKDVEEKLINIYKATQHKRQPIPTIYD

Sequences:

>Translated_296_residues
MITLAQSLLPITANIKVEFSVKAQILTEMKVLKTIDPEFEVQRRVAFIKSKLQAARSKALVLGISGGVDSSTAGRLCQLA
VDSLNSEHTDGGYQFIAVRLPYQIQKDEHEAQQACQFIQPTKLVTINVHQGVDGVHQATLTAFVEAGLHSPDAAKVDFIK
GNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQVAAYLGAPESLVYKAPTADL
EDNQPLLEDEVALGLTYEQIDDFLEGKEVGKDVEEKLINIYKATQHKRQPIPTIYD
>Mature_296_residues
MITLAQSLLPITANIKVEFSVKAQILTEMKVLKTIDPEFEVQRRVAFIKSKLQAARSKALVLGISGGVDSSTAGRLCQLA
VDSLNSEHTDGGYQFIAVRLPYQIQKDEHEAQQACQFIQPTKLVTINVHQGVDGVHQATLTAFVEAGLHSPDAAKVDFIK
GNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQVAAYLGAPESLVYKAPTADL
EDNQPLLEDEVALGLTYEQIDDFLEGKEVGKDVEEKLINIYKATQHKRQPIPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=279, Percent_Identity=49.8207885304659, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 32435; Mature: 32435

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLAQSLLPITANIKVEFSVKAQILTEMKVLKTIDPEFEVQRRVAFIKSKLQAARSKAL
CCCCCCHHCCEEEEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEE
VLGISGGVDSSTAGRLCQLAVDSLNSEHTDGGYQFIAVRLPYQIQKDEHEAQQACQFIQP
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCCHHHHHHHHHHCCC
TKLVTINVHQGVDGVHQATLTAFVEAGLHSPDAAKVDFIKGNVKARMRMIAQYELAGLVG
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHC
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQVAAYLGAPESLVYKAPTADL
CEEEECCCCCCCCEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHEEEECCCCCC
EDNQPLLEDEVALGLTYEQIDDFLEGKEVGKDVEEKLINIYKATQHKRQPIPTIYD
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MITLAQSLLPITANIKVEFSVKAQILTEMKVLKTIDPEFEVQRRVAFIKSKLQAARSKAL
CCCCCCHHCCEEEEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEE
VLGISGGVDSSTAGRLCQLAVDSLNSEHTDGGYQFIAVRLPYQIQKDEHEAQQACQFIQP
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCCHHHHHHHHHHCCC
TKLVTINVHQGVDGVHQATLTAFVEAGLHSPDAAKVDFIKGNVKARMRMIAQYELAGLVG
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHC
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQVAAYLGAPESLVYKAPTADL
CEEEECCCCCCCCEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHEEEECCCCCC
EDNQPLLEDEVALGLTYEQIDDFLEGKEVGKDVEEKLINIYKATQHKRQPIPTIYD
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA