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The map label for this gene is gap3 [H]

Identifier: 117920773

GI number: 117920773

Start: 2806888

End: 2807898

Strand: Reverse

Name: gap3 [H]

Synonym: Shewana3_2330

Alternate gene names: 117920773

Gene position: 2807898-2806888 (Counterclockwise)

Preceding gene: 117920774

Following gene: 117920772

Centisome position: 56.47

GC content: 50.15

Gene sequence:

>1011_bases
ATGACAATCAAAATTGGGATCAATGGCTTTGGCCGTATGGGACGTTTAGCGCTGCGCGCCGCTTGGGGCTGGAATGACGT
CGAATTTGTGCAAATTAACGATCCCGCAGGGGATGCACCGACCTTAGCCCATTTGCTGACATTCGATTCTGTGCATGGCC
GTTGGCAGCATGAGGCGAACAGTGAGGGTGATGACATCATTATCGATGGCAAGCGTATTCGCTGTACGCGCAATAAAACC
ATCGCCGAAACCGATTGGTCTGCCTGCGATGTGGTGATTGAAGCTTCCGGTAAGATGAAGACCAAAGCCGTGCTGCAAGC
TTATTTAGATCAAGGCGTTAAACGTGTTGTGGTGACGGCACCTGTTAAGGAAGAGGGCGTGTTAAACATCGTCATGGGAG
TGAATCATCAGCTCTATGATAAAGCCATTCATCCGATTGTGACGGCGGCATCTTGTACCACTAACTGTTTGGCGCCAATT
GTTAAAGTCATCCACGAAAACCTCGGTATAGTGCATGGTTCGATGACGACCATTCACGATATTACCAACACTCAAACGAT
TTTAGATGCACCGCACAAAGATCTTCGCCGCGCGCGGGCCTGTGGTTTAAGCCTTATCCCCACAACGACGGGCTCAGCGA
CGGCCATTACCCATATTTTCCCTGAACTCAAGGGTAAGCTAAACGGCCATGCGGTGCGCGTACCTCTGGCAAATGCATCT
TTAACCGACTGTGTATTTGAGGTGAGTCGTAAAACCACCGAAGCTGAGGTCAATCGCCTGTTAAAAGAGGCCGCAGAAGG
GCCATTAAAAGGCATTTTAGGTTATGAGGAACGCCCGTTAGTGTCGGTGGATTATAAAACCGATCCCCGTTCGAGCATTA
TCGATGCGCTGTCGACCATGATCATCAATGGCACTCAGGTCAAACTCTACGCTTGGTATGATAACGAGTGGGGTTATGCT
AACCGTGCGGCCGAGCTGGCCCGTATGGTCGGACTGATGGATAAGGCATAA

Upstream 100 bases:

>100_bases
GCTATATCGACCAGTTAACGCTTTAGCATAAGAGTGACGCTTTCATCTTAAACAATAATATCAAAAAGATACGATAAAAC
TGAGTGTGAAGGAAACAGGT

Downstream 100 bases:

>100_bases
GTTATTATATTTAAAGGGATACTATGGCTAAGCTGACAGGGTTATTGTCCAACTTATCACCCGACATTCGCCAGTATTTA
ATGATCACTGGCAACTATTG

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTIKIGINGFGRMGRLALRAAWGWNDVEFVQINDPAGDAPTLAHLLTFDSVHGRWQHEANSEGDDIIIDGKRIRCTRNKT
IAETDWSACDVVIEASGKMKTKAVLQAYLDQGVKRVVVTAPVKEEGVLNIVMGVNHQLYDKAIHPIVTAASCTTNCLAPI
VKVIHENLGIVHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
LTDCVFEVSRKTTEAEVNRLLKEAAEGPLKGILGYEERPLVSVDYKTDPRSSIIDALSTMIINGTQVKLYAWYDNEWGYA
NRAAELARMVGLMDKA

Sequences:

>Translated_336_residues
MTIKIGINGFGRMGRLALRAAWGWNDVEFVQINDPAGDAPTLAHLLTFDSVHGRWQHEANSEGDDIIIDGKRIRCTRNKT
IAETDWSACDVVIEASGKMKTKAVLQAYLDQGVKRVVVTAPVKEEGVLNIVMGVNHQLYDKAIHPIVTAASCTTNCLAPI
VKVIHENLGIVHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
LTDCVFEVSRKTTEAEVNRLLKEAAEGPLKGILGYEERPLVSVDYKTDPRSSIIDALSTMIINGTQVKLYAWYDNEWGYA
NRAAELARMVGLMDKA
>Mature_335_residues
TIKIGINGFGRMGRLALRAAWGWNDVEFVQINDPAGDAPTLAHLLTFDSVHGRWQHEANSEGDDIIIDGKRIRCTRNKTI
AETDWSACDVVIEASGKMKTKAVLQAYLDQGVKRVVVTAPVKEEGVLNIVMGVNHQLYDKAIHPIVTAASCTTNCLAPIV
KVIHENLGIVHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANASL
TDCVFEVSRKTTEAEVNRLLKEAAEGPLKGILGYEERPLVSVDYKTDPRSSIIDALSTMIINGTQVKLYAWYDNEWGYAN
RAAELARMVGLMDKA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=327, Percent_Identity=42.2018348623853, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI7657116, Length=336, Percent_Identity=39.5833333333333, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=43.161094224924, Blast_Score=269, Evalue=1e-73,
Organism=Escherichia coli, GI1789295, Length=328, Percent_Identity=39.0243902439024, Blast_Score=237, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17534677, Length=330, Percent_Identity=42.1212121212121, Blast_Score=273, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17534679, Length=330, Percent_Identity=41.5151515151515, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI32566163, Length=330, Percent_Identity=42.1212121212121, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17568413, Length=330, Percent_Identity=42.1212121212121, Blast_Score=270, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=42.2960725075529, Blast_Score=271, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=41.6918429003021, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=42.042042042042, Blast_Score=266, Evalue=2e-72,
Organism=Drosophila melanogaster, GI19922412, Length=337, Percent_Identity=39.7626112759644, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=40.1197604790419, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=40.1197604790419, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=40.1197604790419, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=40.1197604790419, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36715; Mature: 36583

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGINGFGRMGRLALRAAWGWNDVEFVQINDPAGDAPTLAHLLTFDSVHGRWQHEAN
CEEEEECCCCCCHHHEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
SEGDDIIIDGKRIRCTRNKTIAETDWSACDVVIEASGKMKTKAVLQAYLDQGVKRVVVTA
CCCCEEEECCCEEEEECCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNIVMGVNHQLYDKAIHPIVTAASCTTNCLAPIVKVIHENLGIVHGSMTTIHD
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEC
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCH
LTDCVFEVSRKTTEAEVNRLLKEAAEGPLKGILGYEERPLVSVDYKTDPRSSIIDALSTM
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
IINGTQVKLYAWYDNEWGYANRAAELARMVGLMDKA
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIKIGINGFGRMGRLALRAAWGWNDVEFVQINDPAGDAPTLAHLLTFDSVHGRWQHEAN
EEEEECCCCCCHHHEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
SEGDDIIIDGKRIRCTRNKTIAETDWSACDVVIEASGKMKTKAVLQAYLDQGVKRVVVTA
CCCCEEEECCCEEEEECCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNIVMGVNHQLYDKAIHPIVTAASCTTNCLAPIVKVIHENLGIVHGSMTTIHD
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEC
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCH
LTDCVFEVSRKTTEAEVNRLLKEAAEGPLKGILGYEERPLVSVDYKTDPRSSIIDALSTM
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
IINGTQVKLYAWYDNEWGYANRAAELARMVGLMDKA
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]