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The map label for this gene is aglA [H]

Identifier: 117920754

GI number: 117920754

Start: 2778452

End: 2780074

Strand: Reverse

Name: aglA [H]

Synonym: Shewana3_2311

Alternate gene names: 117920754

Gene position: 2780074-2778452 (Counterclockwise)

Preceding gene: 117920760

Following gene: 117920753

Centisome position: 55.91

GC content: 49.91

Gene sequence:

>1623_bases
ATGAGTGAGTTAACTTGGTGGCGTGGAGCCGTGATCTATCAAATCTATCCACGTAGCCTTTTGGATACTAATGGTGATGG
CGTTGGCGATCTGCGCGGTATTATCACTAAGTTGGACTATATTGCCAGCCTGAATGTGGATGCGATTTGGATTTCGCCAT
TCTTTAAATCCCCGATGGCCGACTTTGGTTACGATATCAGTGACTACCGCGAGGTGGATCCTCTGTTTGGCACTATGCAG
GATTTTGATGAACTGATTGAAAAAGCCCATCAACGTGGCATTAAAGTCATTATCGACCAAGTGCTAAGCCATACCTCAGA
TCAACATGCGTGGTTTTGTGAGAGTCGCGAGAGCCGCACCAATCCCAAGGCAGATTGGTATGTGTGGGCCGAACCAAAGG
AAGATGGTACGGCGCCAAACAACTGGTTAGCGATTTTCGGCGGTTGCGCCTGGGAGTGGGAGCCGCGCAGACAACAATAT
TATCTGCACAACTTTTTACGCAGCCAACCCGATATCAACTTCCACAATCCCGACGTGCGTCAAGCCGTGTTAGATAATGT
GGAGTTTTGGCTGAAGAAGGGCGTCGATGGTTTCCGCTTAGATGCCATTACCTTCTGTTATCATGATGAACAGCTTAGGG
ATAATCCGCCCAAACCCAAGGATAAACGCCAAGGGCGCGGATTCAGTGAAGATAATCCCTATGCCTATCAGTACCATTAC
TACAATAACGATAGACCGCAAACGATTCTATTTATTGAAGAATTACGCCAGTTAATCAATCGCTATCCAGGTGCTGTGAC
CTTAGGGGAAGTCTCCGCCGAGGATTCACTTGCGGTGATGGCCGCCTATACCAAGGGCGAAGACCGCTTACATATGGCCT
ACAGTTTCGAGCTGCTGACCGATGACTATAGCGCCGCTTATATTCGCCAAACGGTAGAAGCACTGGAAGCGAGTATTGGT
GATGGTTGGCCCTGCTGGGCGATAGGTAATCACGATGCACAGCGCGTCGCCAGCCGTTGGGGACGTGGTAAGCAAACATC
CGACATGGTGAAAATGCTCAATGCCATGGTTAACTCACTGCGAGGCAGTGTCTGTAGCTATCAAGGAGAAGAGCTGGGTT
TAACCGAAGCGCCGATTGAATACCACGAGTTGCAGGATCCCTTCGGCAAAACCTTCTGGCCAATGTTTAAGGGCCGTGAT
GGCTGCCGTACACCTATGCCGTGGGAGCAAAATGCAGATTTTAGCGGCTTTAGCCAAGTGACACCTTGGTTGCCTATCGC
GCAGGCGCACAGAGCACTGGCGGTTGATGTGCAGGAAGCCGATAGCCAATCGATGTTACATGGCTATCGTCAGTTCCTCG
CATGGCGCAAAGGCTATCCCGCTTTGGTTGAAGGGGAAATTGAATTCCTCGATGCGCCAGAACCCTTGTTGGTGTTTGTG
CGTACGTCCGGGGAGCAAAAGCTACTGGTGTGCTTTAACCTACAAGATACAGAGCAGGCATTATCACTGGATGCACTCAG
CATCCAGCAAGAAATTACCGGTCACGGTTTGAAAACTGCGCAAAGGATCGCCGACACATTGACATTTTCGGCCTATGCCT
GCTTTTATGCGTTGTTGAACTAG

Upstream 100 bases:

>100_bases
ATACAAGCCGTAGCGCCTTTACTCCGCAGTAACATGATTGTAACTGCTCATGTGAGAGTTTTTGGGTTAGCGCGCTGAAG
AATAAGGATAAAGGGGCAAT

Downstream 100 bases:

>100_bases
TTTCGACTCGGCATAACACCACTTCGGATGGGCAGCACAATTTCATTGTGGCTGCCTGTCGTAGTACGAAAACGCCTAAG
GGACATTCCCTTTAGATAAT

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMQ
DFDELIEKAHQRGIKVIIDQVLSHTSDQHAWFCESRESRTNPKADWYVWAEPKEDGTAPNNWLAIFGGCAWEWEPRRQQY
YLHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPPKPKDKRQGRGFSEDNPYAYQYHY
YNNDRPQTILFIEELRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIG
DGWPCWAIGNHDAQRVASRWGRGKQTSDMVKMLNAMVNSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRD
GCRTPMPWEQNADFSGFSQVTPWLPIAQAHRALAVDVQEADSQSMLHGYRQFLAWRKGYPALVEGEIEFLDAPEPLLVFV
RTSGEQKLLVCFNLQDTEQALSLDALSIQQEITGHGLKTAQRIADTLTFSAYACFYALLN

Sequences:

>Translated_540_residues
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMQ
DFDELIEKAHQRGIKVIIDQVLSHTSDQHAWFCESRESRTNPKADWYVWAEPKEDGTAPNNWLAIFGGCAWEWEPRRQQY
YLHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPPKPKDKRQGRGFSEDNPYAYQYHY
YNNDRPQTILFIEELRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIG
DGWPCWAIGNHDAQRVASRWGRGKQTSDMVKMLNAMVNSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRD
GCRTPMPWEQNADFSGFSQVTPWLPIAQAHRALAVDVQEADSQSMLHGYRQFLAWRKGYPALVEGEIEFLDAPEPLLVFV
RTSGEQKLLVCFNLQDTEQALSLDALSIQQEITGHGLKTAQRIADTLTFSAYACFYALLN
>Mature_539_residues
SELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMADFGYDISDYREVDPLFGTMQD
FDELIEKAHQRGIKVIIDQVLSHTSDQHAWFCESRESRTNPKADWYVWAEPKEDGTAPNNWLAIFGGCAWEWEPRRQQYY
LHNFLRSQPDINFHNPDVRQAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPPKPKDKRQGRGFSEDNPYAYQYHYY
NNDRPQTILFIEELRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLTDDYSAAYIRQTVEALEASIGD
GWPCWAIGNHDAQRVASRWGRGKQTSDMVKMLNAMVNSLRGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDG
CRTPMPWEQNADFSGFSQVTPWLPIAQAHRALAVDVQEADSQSMLHGYRQFLAWRKGYPALVEGEIEFLDAPEPLLVFVR
TSGEQKLLVCFNLQDTEQALSLDALSIQQEITGHGLKTAQRIADTLTFSAYACFYALLN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=525, Percent_Identity=32.7619047619048, Blast_Score=285, Evalue=1e-76,
Organism=Homo sapiens, GI65506891, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744475, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744479, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744477, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744483, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744481, Length=132, Percent_Identity=30.3030303030303, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790687, Length=551, Percent_Identity=34.3012704174229, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1786604, Length=487, Percent_Identity=24.2299794661191, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789995, Length=91, Percent_Identity=38.4615384615385, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32565753, Length=513, Percent_Identity=23.9766081871345, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25147709, Length=487, Percent_Identity=22.9979466119097, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322245, Length=552, Percent_Identity=33.5144927536232, Blast_Score=286, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6319776, Length=556, Percent_Identity=34.5323741007194, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6321731, Length=556, Percent_Identity=33.9928057553957, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6321726, Length=582, Percent_Identity=32.8178694158076, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6324416, Length=545, Percent_Identity=33.0275229357798, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6322241, Length=545, Percent_Identity=33.0275229357798, Blast_Score=259, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6322021, Length=545, Percent_Identity=33.0275229357798, Blast_Score=259, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24583749, Length=531, Percent_Identity=37.4764595103578, Blast_Score=324, Evalue=8e-89,
Organism=Drosophila melanogaster, GI24583747, Length=525, Percent_Identity=37.7142857142857, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24586591, Length=520, Percent_Identity=36.1538461538462, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24586587, Length=517, Percent_Identity=37.330754352031, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24586593, Length=521, Percent_Identity=35.700575815739, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24583745, Length=556, Percent_Identity=34.5323741007194, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24586589, Length=558, Percent_Identity=34.2293906810036, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI221330053, Length=545, Percent_Identity=33.9449541284404, Blast_Score=298, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24586599, Length=507, Percent_Identity=33.9250493096647, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI45549022, Length=558, Percent_Identity=32.078853046595, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24586597, Length=533, Percent_Identity=34.1463414634146, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI281360393, Length=464, Percent_Identity=33.1896551724138, Blast_Score=237, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61888; Mature: 61757

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMA
CCCCCHHCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCHH
DFGYDISDYREVDPLFGTMQDFDELIEKAHQRGIKVIIDQVLSHTSDQHAWFCESRESRT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCC
NPKADWYVWAEPKEDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLRSQPDINFHNPDVR
CCCCCEEEEECCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
QAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPPKPKDKRQGRGFSEDNPYAYQYHY
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNDRPQTILFIEELRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLT
ECCCCCCEEHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCEEEEEEEEEEEC
DDYSAAYIRQTVEALEASIGDGWPCWAIGNHDAQRVASRWGRGKQTSDMVKMLNAMVNSL
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDGCRTPMPWEQNADFSGFSQV
CCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
TPWLPIAQAHRALAVDVQEADSQSMLHGYRQFLAWRKGYPALVEGEIEFLDAPEPLLVFV
CCCCCHHHCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCCEEEEE
RTSGEQKLLVCFNLQDTEQALSLDALSIQQEITGHGLKTAQRIADTLTFSAYACFYALLN
EECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELTWWRGAVIYQIYPRSLLDTNGDGVGDLRGIITKLDYIASLNVDAIWISPFFKSPMA
CCCCHHCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCHH
DFGYDISDYREVDPLFGTMQDFDELIEKAHQRGIKVIIDQVLSHTSDQHAWFCESRESRT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCC
NPKADWYVWAEPKEDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLRSQPDINFHNPDVR
CCCCCEEEEECCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
QAVLDNVEFWLKKGVDGFRLDAITFCYHDEQLRDNPPKPKDKRQGRGFSEDNPYAYQYHY
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNDRPQTILFIEELRQLINRYPGAVTLGEVSAEDSLAVMAAYTKGEDRLHMAYSFELLT
ECCCCCCEEHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCCEEEEEEEEEEEC
DDYSAAYIRQTVEALEASIGDGWPCWAIGNHDAQRVASRWGRGKQTSDMVKMLNAMVNSL
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RGSVCSYQGEELGLTEAPIEYHELQDPFGKTFWPMFKGRDGCRTPMPWEQNADFSGFSQV
CCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
TPWLPIAQAHRALAVDVQEADSQSMLHGYRQFLAWRKGYPALVEGEIEFLDAPEPLLVFV
CCCCCHHHCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCCEEEEE
RTSGEQKLLVCFNLQDTEQALSLDALSIQQEITGHGLKTAQRIADTLTFSAYACFYALLN
EECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]