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The map label for this gene is fumB [H]

Identifier: 117920745

GI number: 117920745

Start: 2766330

End: 2767874

Strand: Reverse

Name: fumB [H]

Synonym: Shewana3_2302

Alternate gene names: 117920745

Gene position: 2767874-2766330 (Counterclockwise)

Preceding gene: 117920748

Following gene: 117920744

Centisome position: 55.67

GC content: 51.2

Gene sequence:

>1545_bases
ATGTCATCTCACCATGAAACGTCTGAAAATGTAGTGATTAAACAAGCCGACTTTATTGAAAGTGTGGCCGATGCCCTGCA
ATACATCTCCTATTATCATCCCAAAGATTTTGTGGATGCCATGAGCGAAGCTTATGAGCGTGAGCAAAGTGCGGCGGCGA
AGGATGCGATTGCGCAAATTTTGATTAACTCGCGTATGTCTGCCGAAGGTAAACGTCCACTGTGTCAAGACACGGGTATT
GTGACCACCTTCGTTAAGATTGGTATGGGGGTTAAGTGGGACAAAACCGATATGACTGTGCAACAAATGGTCGATGAAGG
TGTGCGTCGTGCTTATACCAATCCAGATAACCCACTGCGTGCATCGATTGTGGCCGATCCTGCGGGTAGCCGTAAAAACA
CCAAAGACAACACGCCATCCGTCGTGCATATCGATATGGTGCCAGGCAATCACATTGAAGTCGCTATCGCGGCCAAGGGC
GGCGGCAGTGAAAACAAAGCCAAGATGGTGATGTTAAACCCATCTGACGATATCGCCGCTTGGGTTGAAAAAACGCTGCC
AACCATGGGCGCGGGCTGGTGTCCTCCAGGTATGCTTGGCATCGGTATTGGCGGCACCGCTGAGAAAGCGGCCGTACTCG
CGAAAGAAGCACTGATGGAAAGCGTGGATATCCATGAGTTAATGGCGCGCGGTGCAGAAACCAGCGAAGAAAAACTGCGT
TTAGACATTTTCGAGCGTGCTAACAACCTTGGTATTGGTGCGCAGGGTCTTGGCGGTTTAACCACAGTGTTAGACGTGAA
AATCAAATCTGCACCTACTCATGCAGCATCAAAACCTGTGGTGATGATCCCGAACTGCGCGGCAACTCGTCACGTACACT
TCCATTTAGATGGTTCGGGCCCTGCTGATCTTGCGCCGCCTACACTCAGCGACTGGCCAGAAATCACCCGCGAAGTGGGC
AGCGATGTGCGACGCGTTAACTTAGACACAGTCACTCAAGCCGATATCGAGCAGTGGAAGAGCGGTGAAACCATTCTGTT
AAGCGGTAAAATGCTCACCGGCCGTGATGCTGCCCATAAACGTATCCAAAGCCTGATTGAAAGCGGCGAAGGCTTACCAG
AAGGCGTCGACTTTACTGGCAAGTTTATCTACTACGTGGGCCCTGTTGACCCAGTCGGTAACGAAGTCGTGGGTCCTGCT
GGCCCAACAACCGCGACCCGTATGGACAAGTTCACCGATCTGATGCTGGATAAAACTGGCCTGATGGGCATGATTGGTAA
GGCTGAACGTGGTCCAGCGACCGTTGAGTCTATCAAGAAGCACAAAGCTGTGTACCTGATGGCTGTGGGCGGCGCGGCTT
ACTTAGTGTCGAAAGCCATTAAGAAATCCCGCGTGGTGGCCTTTGCCGACTTAGGTATGGAAGCAATTTACGAGTTTGAC
GTACAAGATATGCCAGTTACTGTGGCGGTGGATTCAAACGGCGTGAATGCCCATGAAACTGGCCCTGCGATTTGGAAAGT
GAATATCGCTAACGCCAAAGCCTAA

Upstream 100 bases:

>100_bases
GTGATGTAGCCCAAAAAGATGGGAAAGAGTATCATATTGGCCCAGAAAGAATTACACAAAAAACAAGTAAGGCCCTAAAG
GCTGCTGGAGCGCACCTACT

Downstream 100 bases:

>100_bases
GCAAATGGCCTAAGCGAATGTCCTTCTTGCAAAAATGTCGCTTTTTTAAGCGGGATTTGAGCGGGAAAGGGCGGCGAGTG
GCACTCAATGACGCCATTCG

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSSHHETSENVVIKQADFIESVADALQYISYYHPKDFVDAMSEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGI
VTTFVKIGMGVKWDKTDMTVQQMVDEGVRRAYTNPDNPLRASIVADPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKG
GGSENKAKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAETSEEKLR
LDIFERANNLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPADLAPPTLSDWPEITREVG
SDVRRVNLDTVTQADIEQWKSGETILLSGKMLTGRDAAHKRIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGNEVVGPA
GPTTATRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFD
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA

Sequences:

>Translated_514_residues
MSSHHETSENVVIKQADFIESVADALQYISYYHPKDFVDAMSEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGI
VTTFVKIGMGVKWDKTDMTVQQMVDEGVRRAYTNPDNPLRASIVADPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKG
GGSENKAKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAETSEEKLR
LDIFERANNLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPADLAPPTLSDWPEITREVG
SDVRRVNLDTVTQADIEQWKSGETILLSGKMLTGRDAAHKRIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGNEVVGPA
GPTTATRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFD
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA
>Mature_513_residues
SSHHETSENVVIKQADFIESVADALQYISYYHPKDFVDAMSEAYEREQSAAAKDAIAQILINSRMSAEGKRPLCQDTGIV
TTFVKIGMGVKWDKTDMTVQQMVDEGVRRAYTNPDNPLRASIVADPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKGG
GSENKAKMVMLNPSDDIAAWVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAETSEEKLRL
DIFERANNLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSGPADLAPPTLSDWPEITREVGS
DVRRVNLDTVTQADIEQWKSGETILLSGKMLTGRDAAHKRIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGNEVVGPAG
PTTATRMDKFTDLMLDKTGLMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFDV
QDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=32.3404255319149, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787897, Length=466, Percent_Identity=33.6909871244635, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789442, Length=280, Percent_Identity=30.3571428571429, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=31.2849162011173, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54972; Mature: 54841

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHHETSENVVIKQADFIESVADALQYISYYHPKDFVDAMSEAYEREQSAAAKDAIAQI
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LINSRMSAEGKRPLCQDTGIVTTFVKIGMGVKWDKTDMTVQQMVDEGVRRAYTNPDNPLR
HHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEE
ASIVADPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKGGGSENKAKMVMLNPSDDIAA
EEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHH
WVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAETSEEKLR
HHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHH
LDIFERANNLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSG
HHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCC
PADLAPPTLSDWPEITREVGSDVRRVNLDTVTQADIEQWKSGETILLSGKMLTGRDAAHK
CCCCCCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHCCCCEEEEECCEECCCHHHHH
RIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGNEVVGPAGPTTATRMDKFTDLMLDKTG
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFD
CHHHCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCHHHHEECC
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA
CCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
SSHHETSENVVIKQADFIESVADALQYISYYHPKDFVDAMSEAYEREQSAAAKDAIAQI
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LINSRMSAEGKRPLCQDTGIVTTFVKIGMGVKWDKTDMTVQQMVDEGVRRAYTNPDNPLR
HHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEE
ASIVADPAGSRKNTKDNTPSVVHIDMVPGNHIEVAIAAKGGGSENKAKMVMLNPSDDIAA
EEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHH
WVEKTLPTMGAGWCPPGMLGIGIGGTAEKAAVLAKEALMESVDIHELMARGAETSEEKLR
HHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHH
LDIFERANNLGIGAQGLGGLTTVLDVKIKSAPTHAASKPVVMIPNCAATRHVHFHLDGSG
HHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECCCC
PADLAPPTLSDWPEITREVGSDVRRVNLDTVTQADIEQWKSGETILLSGKMLTGRDAAHK
CCCCCCCCCCHHHHHHHHHCCHHEEECCCHHHHHHHHHHCCCCEEEEECCEECCCHHHHH
RIQSLIESGEGLPEGVDFTGKFIYYVGPVDPVGNEVVGPAGPTTATRMDKFTDLMLDKTG
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
LMGMIGKAERGPATVESIKKHKAVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFD
CHHHCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCHHHHEECC
VQDMPVTVAVDSNGVNAHETGPAIWKVNIANAKA
CCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]