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The map label for this gene is iscS [H]

Identifier: 117920723

GI number: 117920723

Start: 2742056

End: 2743270

Strand: Reverse

Name: iscS [H]

Synonym: Shewana3_2280

Alternate gene names: 117920723

Gene position: 2743270-2742056 (Counterclockwise)

Preceding gene: 117920724

Following gene: 117920722

Centisome position: 55.17

GC content: 48.4

Gene sequence:

>1215_bases
ATGAAGCTTCCTATCTATTTAGATTATGCCGCCACCACGCCAGTTGATCCGCGAGTCGCGGAAAAAATGTTCCAATACAT
GACGATGGATGGCATCTTCGGTAACCCAGCATCACGTTCTCACCGTTATGGCTGGCAAGCGGAAGAAGCGGTGGATGTGG
CACGCAGCCAAGTGGCTGATCTGATCAATGCCGATCACCGTGAAATCGTGTTTACCTCAGGTGCAACCGAATCAAACAAC
CTGGCGATCAAAGGCGTAGCGCATTTCTACAATAAGAAAGGCAAGCACATCATCACCAGTAAGACTGAACATAAAGCGGT
ACTCGATACCTGCCGTCAGCTTGAGCGTGAAGGCTTTGAAGTCACTTACTTAGAGCCAGAAGCCAACGGCATTATTCCGA
TGGAACGTTTAGAAGCGGCGATGCGCGATGACACCATTCTTGTCAGCATCATGCATGTGAATAACGAAATCGGTGTGATC
CATGATGTGGATGCCATCGGTGAGTTATGCCGCTCAAAAGGCATTATCTTCCATATGGATGCGGCACAAAGCGCGGGTAA
ATTACCTATCGATGTACAAACGACTAAGGTCGACCTGATCTCTATCTCTGGCCACAAAATGTATGGTCCAAAAGGGATTG
GTGCTTTGTACGTTCGCCGTAAGCCACGCATTCGCTTAGAAGCCCAAATGCACGGCGGTGGTCATGAGCGTGGTATGCGC
AGTGGTACACTGGCGACTCACCAAATCGTCGGTTTAGGTGAAGCGGCTGCAATCGCAAAAGCTGAAATGGCAACGGACAA
CGCCCGTATCGCCAAGCTGCGTGACAAGTTATGGAATGGCATCAAGCACATCGAAGAGACGTATGTAAACGGCGACATGA
CTCACCGTGTGAGCGGTAGCCTGAACGTCAGCTTCAACTATGTTGAAGGTGAGTCGCTGATGATGGCATTAAAAGATTTA
GCGGTTTCATCGGGTTCTGCCTGTACTTCAGCCAGTCTCGAGCCAAGCTACGTGTTACGTGCGCTCGGTTTAAATGATGA
AATGGCGCATAGCTCAATTCGTTTCTCAATCGGTCGTTTTACCACTGAAGAAGAAATCGACCACGCTATCGAAACTATTA
CACAATCTATTGATAAATTAAGAGAAATGTCTCCATTGTGGGAGATGTTCAAAGATGGCATCGACCTGAATCAGGTCCAG
TGGGCCCATCATTAA

Upstream 100 bases:

>100_bases
GGAGCAAAGAGTCAGCTTATAGGCTTTTTGTTATACGATAAAAATTATTTTTGTACGTTGTATGTAGCCTCGGCAGAGAC
TGCTAAGTACGGAGTGTGTT

Downstream 100 bases:

>100_bases
TTCGACCGTTTACAATAGATAATTTTGGAGCAGTACCATGGCTTACAGTGAAAAAGTGATAGATCATTATGAGAACCCAC
GTAACGTTGGTTCTTTCGAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVADLINADHREIVFTSGATESNN
LAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPEANGIIPMERLEAAMRDDTILVSIMHVNNEIGVI
HDVDAIGELCRSKGIIFHMDAAQSAGKLPIDVQTTKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLATHQIVGLGEAAAIAKAEMATDNARIAKLRDKLWNGIKHIEETYVNGDMTHRVSGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVADLINADHREIVFTSGATESNN
LAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPEANGIIPMERLEAAMRDDTILVSIMHVNNEIGVI
HDVDAIGELCRSKGIIFHMDAAQSAGKLPIDVQTTKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLATHQIVGLGEAAAIAKAEMATDNARIAKLRDKLWNGIKHIEETYVNGDMTHRVSGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH
>Mature_404_residues
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVADLINADHREIVFTSGATESNN
LAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLEPEANGIIPMERLEAAMRDDTILVSIMHVNNEIGVI
HDVDAIGELCRSKGIIFHMDAAQSAGKLPIDVQTTKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLATHQIVGLGEAAAIAKAEMATDNARIAKLRDKLWNGIKHIEETYVNGDMTHRVSGSLNVSFNYVEGESLMMALKDL
AVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRFTTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQ
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=59.7014925373134, Blast_Score=521, Evalue=1e-148,
Organism=Homo sapiens, GI156713448, Length=421, Percent_Identity=32.7790973871734, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.960396039604, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=181, Percent_Identity=33.1491712707182, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=31.1627906976744, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=61.2903225806452, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17533177, Length=334, Percent_Identity=33.2335329341317, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=58.354114713217, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=58.7064676616915, Blast_Score=499, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44804; Mature: 44804

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVAD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCE
VTYLEPEANGIIPMERLEAAMRDDTILVSIMHVNNEIGVIHDVDAIGELCRSKGIIFHMD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEC
AAQSAGKLPIDVQTTKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEEEEECCCCHHCCCC
SGTLATHQIVGLGEAAAIAKAEMATDNARIAKLRDKLWNGIKHIEETYVNGDMTHRVSGS
CCCCHHHHEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCE
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEEECCC
TTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
>Mature Secondary Structure
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARSQVAD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LINADHREIVFTSGATESNNLAIKGVAHFYNKKGKHIITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCE
VTYLEPEANGIIPMERLEAAMRDDTILVSIMHVNNEIGVIHDVDAIGELCRSKGIIFHMD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEC
AAQSAGKLPIDVQTTKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
CCCCCCCCCEEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEEEEEEECCCCHHCCCC
SGTLATHQIVGLGEAAAIAKAEMATDNARIAKLRDKLWNGIKHIEETYVNGDMTHRVSGS
CCCCHHHHEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCE
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEEECCC
TTEEEIDHAIETITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA