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The map label for this gene is nth [H]

Identifier: 117920618

GI number: 117920618

Start: 2606780

End: 2607415

Strand: Direct

Name: nth [H]

Synonym: Shewana3_2174

Alternate gene names: 117920618

Gene position: 2606780-2607415 (Clockwise)

Preceding gene: 117920617

Following gene: 117920619

Centisome position: 52.43

GC content: 49.21

Gene sequence:

>636_bases
ATGAATCAAGAAAAACGCATCCAAATCCTCACCCGCCTTCGCGAAAACAATCCCAAACCCGAAACCGAGCTGAATTTCTC
CAGTCCCTTTGAATTACTGGTGGCGGTGACTTTATCAGCGCAGGCGACCGATGTGAGCGTGAATAAGGCGACCGATAAAC
TGTTTCCAGTCGCCAATACCGCCCACAGCATTTATGCCTTAGGCGTGGATGGATTAAAGGAATATATCAAGACCATTGGC
CTGTATAACAACAAGGCCATCAATGTGATTAAAGCCTGTGAGATCTTGATTAAAAAATACAATGGCGAAGTCCCAGAGGA
CAGAGAAGCGCTAGAGTCGCTTCCTGGTGTCGGCCGTAAAACCGCTAACGTGGTGCTCAATACCGCCTTTGGCTGGCCAA
CCATCGCCGTCGATACCCATATCTTTCGCATGGCAAACCGCACTAAATTCGCCCCCGGTAAAAACGTGGTCGAAGTCGAG
GAGCGCATGTTAAAGGTTGTTCCCGCCGAATTTAAAGTCGATGTGCACCACTGGTTTATTCTGCATGGCCGCTATACCTG
TCTGGCCCGCAAACCCCGCTGTGGCAGCTGCATTATTGAGGACCTTTGCGAATACTCCCCACTAAAAGAAAAGTAA

Upstream 100 bases:

>100_bases
TGTAACTCGGGCACGGATCACCAAAGTGAGTTAAGCCCTTATTGAGATAACAGCTTAAGTAGTGGGTTTAGCGTTAAACC
CACTGCTTACAAGGATCACT

Downstream 100 bases:

>100_bases
AGAATCAGACTTAGTTTGTATAACAGTTTCCTTAGTAAATCTGTTTTTTATCTTTACAAAAAGCTAAAGACCTACCATAG
TGTATTTATGTGTAAGCGAT

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNQEKRIQILTRLRENNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVDGLKEYIKTIG
LYNNKAINVIKACEILIKKYNGEVPEDREALESLPGVGRKTANVVLNTAFGWPTIAVDTHIFRMANRTKFAPGKNVVEVE
ERMLKVVPAEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEYSPLKEK

Sequences:

>Translated_211_residues
MNQEKRIQILTRLRENNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVDGLKEYIKTIG
LYNNKAINVIKACEILIKKYNGEVPEDREALESLPGVGRKTANVVLNTAFGWPTIAVDTHIFRMANRTKFAPGKNVVEVE
ERMLKVVPAEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEYSPLKEK
>Mature_211_residues
MNQEKRIQILTRLRENNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVDGLKEYIKTIG
LYNNKAINVIKACEILIKKYNGEVPEDREALESLPGVGRKTANVVLNTAFGWPTIAVDTHIFRMANRTKFAPGKNVVEVE
ERMLKVVPAEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEYSPLKEK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.9435028248588, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=80.4878048780488, Blast_Score=354, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=29.8342541436464, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.1336898395722, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=32.5842696629214, Blast_Score=98, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23755; Mature: 23755

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEKRIQILTRLRENNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEECCCCCEEECHHHHHHHCCCCCC
AHSIYALGVDGLKEYIKTIGLYNNKAINVIKACEILIKKYNGEVPEDREALESLPGVGRK
CCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRMANRTKFAPGKNVVEVEERMLKVVPAEFKVDVHHWFI
HHHEEEECCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEEEEEE
LHGRYTCLARKPRCGSCIIEDLCEYSPLKEK
EECCEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNQEKRIQILTRLRENNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEECCCCCEEECHHHHHHHCCCCCC
AHSIYALGVDGLKEYIKTIGLYNNKAINVIKACEILIKKYNGEVPEDREALESLPGVGRK
CCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRMANRTKFAPGKNVVEVEERMLKVVPAEFKVDVHHWFI
HHHEEEECCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEEEEEE
LHGRYTCLARKPRCGSCIIEDLCEYSPLKEK
EECCEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]