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The map label for this gene is ybfF [H]

Identifier: 117920580

GI number: 117920580

Start: 2562280

End: 2563080

Strand: Direct

Name: ybfF [H]

Synonym: Shewana3_2136

Alternate gene names: 117920580

Gene position: 2562280-2563080 (Clockwise)

Preceding gene: 117920577

Following gene: 117920581

Centisome position: 51.53

GC content: 49.06

Gene sequence:

>801_bases
ATGTACCACAGCACAGGCGATTCCATGAATTTTGCATCCACAGGTCAAGGAGAAGAAGTCCTCCTTATCCACGGACTCTT
TGGCAATTTAGATAATCTTAAGGGGCTAGGACAAGTCCTCGAAGCAAATCATCAGGTTATCCGCGTTGATGTGCCTAATC
ATGGTTTGAGTGAACATTGGCAGGAGATGGATTACCCAAGCTTAGCCAAGGCCATGGTGGCATTGTTAGATGAACTTGAG
CTTGAGCGTATACACATTGTTGGCCATTCCATGGGCGGTAAAATCGCCATGGCAACCGCCCTTGCCTATCCAGAACGCAT
CATCAGCATGGTAGCCGCCGATATTGCCCCAGTTGCTTACCAGCCGCGCCATGATGCGGTATTTGCGGCACTCGAAAGTT
TGCCACTCGAAGGCCACACCGACCGCCGCTTCGCGTTATCGCATTTATTAGCGGGTGGCATCGATGAACCGACCGCGCAG
TTTTTACTCAAAAACCTCCAGCGCACCGACACCGGCTTTCGTTGGAAGATGAACCTTACTGGTTTAAAAAGCTGTTATCC
CAACATCATTGGTTGGCCAAATGAAAACCACAAATCGCAGCAAGTCTATAACGGACCGAGCCTTTTTATCCGTGGTGGCG
ACTCCAATTATGTGACCGCCGAACATCGCGATGAGATCATGCGTCAATTTCCCGCGGCGCAGGCCAAAACATTAGAAGGT
TGTGGCCATTGGCTGCATGCTCAAAAACCAGCGGTGTTTAATCGTATTGTGTCTGAATTTATTGACAAACACTCAGTGTA
A

Upstream 100 bases:

>100_bases
TTAATCGCTGAAAAAGCCAGCAAGTTCGGCGCACTGTGCCTCAAAATCGCCTCAAGACTTGTCAAACTTAACCCATTAGG
GGCACAATCCGCAGCACTTT

Downstream 100 bases:

>100_bases
ATGACAATGATTCTCGACCTAAATCATATAGCCGACACCCTGCTGATGTGATATATTCGCGCCTCTTTTAGCCTTAGAAG
ACTCTAGAGGACTAAGAAAG

Product: alpha/beta hydrolase fold domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MYHSTGDSMNFASTGQGEEVLLIHGLFGNLDNLKGLGQVLEANHQVIRVDVPNHGLSEHWQEMDYPSLAKAMVALLDELE
LERIHIVGHSMGGKIAMATALAYPERIISMVAADIAPVAYQPRHDAVFAALESLPLEGHTDRRFALSHLLAGGIDEPTAQ
FLLKNLQRTDTGFRWKMNLTGLKSCYPNIIGWPNENHKSQQVYNGPSLFIRGGDSNYVTAEHRDEIMRQFPAAQAKTLEG
CGHWLHAQKPAVFNRIVSEFIDKHSV

Sequences:

>Translated_266_residues
MYHSTGDSMNFASTGQGEEVLLIHGLFGNLDNLKGLGQVLEANHQVIRVDVPNHGLSEHWQEMDYPSLAKAMVALLDELE
LERIHIVGHSMGGKIAMATALAYPERIISMVAADIAPVAYQPRHDAVFAALESLPLEGHTDRRFALSHLLAGGIDEPTAQ
FLLKNLQRTDTGFRWKMNLTGLKSCYPNIIGWPNENHKSQQVYNGPSLFIRGGDSNYVTAEHRDEIMRQFPAAQAKTLEG
CGHWLHAQKPAVFNRIVSEFIDKHSV
>Mature_266_residues
MYHSTGDSMNFASTGQGEEVLLIHGLFGNLDNLKGLGQVLEANHQVIRVDVPNHGLSEHWQEMDYPSLAKAMVALLDELE
LERIHIVGHSMGGKIAMATALAYPERIISMVAADIAPVAYQPRHDAVFAALESLPLEGHTDRRFALSHLLAGGIDEPTAQ
FLLKNLQRTDTGFRWKMNLTGLKSCYPNIIGWPNENHKSQQVYNGPSLFIRGGDSNYVTAEHRDEIMRQFPAAQAKTLEG
CGHWLHAQKPAVFNRIVSEFIDKHSV

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=250, Percent_Identity=36.4, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI23200008, Length=250, Percent_Identity=36.4, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI223941839, Length=244, Percent_Identity=31.5573770491803, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786902, Length=256, Percent_Identity=37.890625, Blast_Score=177, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17508535, Length=270, Percent_Identity=31.1111111111111, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17507063, Length=263, Percent_Identity=27.7566539923954, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321468, Length=267, Percent_Identity=29.2134831460674, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24640348, Length=266, Percent_Identity=31.203007518797, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24646055, Length=259, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 29557; Mature: 29557

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHSTGDSMNFASTGQGEEVLLIHGLFGNLDNLKGLGQVLEANHQVIRVDVPNHGLSEHW
CCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
QEMDYPSLAKAMVALLDELELERIHIVGHSMGGKIAMATALAYPERIISMVAADIAPVAY
HHCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
QPRHDAVFAALESLPLEGHTDRRFALSHLLAGGIDEPTAQFLLKNLQRTDTGFRWKMNLT
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECC
GLKSCYPNIIGWPNENHKSQQVYNGPSLFIRGGDSNYVTAEHRDEIMRQFPAAQAKTLEG
HHHHHCCCCCCCCCCCCCCCEEECCCEEEEECCCCCEECHHHHHHHHHHCCCHHHHHHHH
CGHWLHAQKPAVFNRIVSEFIDKHSV
HHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYHSTGDSMNFASTGQGEEVLLIHGLFGNLDNLKGLGQVLEANHQVIRVDVPNHGLSEHW
CCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
QEMDYPSLAKAMVALLDELELERIHIVGHSMGGKIAMATALAYPERIISMVAADIAPVAY
HHCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
QPRHDAVFAALESLPLEGHTDRRFALSHLLAGGIDEPTAQFLLKNLQRTDTGFRWKMNLT
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECC
GLKSCYPNIIGWPNENHKSQQVYNGPSLFIRGGDSNYVTAEHRDEIMRQFPAAQAKTLEG
HHHHHCCCCCCCCCCCCCCCEEECCCEEEEECCCCCEECHHHHHHHHHHCCCHHHHHHHH
CGHWLHAQKPAVFNRIVSEFIDKHSV
HHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]