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The map label for this gene is seqA [H]

Identifier: 117920579

GI number: 117920579

Start: 2561457

End: 2562008

Strand: Reverse

Name: seqA [H]

Synonym: Shewana3_2135

Alternate gene names: 117920579

Gene position: 2562008-2561457 (Counterclockwise)

Preceding gene: 117920586

Following gene: 117920578

Centisome position: 51.53

GC content: 49.64

Gene sequence:

>552_bases
ATGAAATACATCGAAGTGGATGAAGAGCTCTATCGTCATATTGCCAGCAAAACGGAACGTATTGGTGAGAGCGCCTCTGA
CATTCTCCGTCGATTACTCGGCCTGTCCGTCGATGCGGTTGAACAGGCGCAGCCACAGGCCATTAGCCAACCGAGTTTAG
AGGCAACGACGCCTGAGCCACAGATAGCACCTCAGATAGAAACCTTAGTCTCTGCGACCGATTTCAATCAATTGGTGGAT
GAGCATAAGTTAGAGCAGCAAAAAGGCGCCGTGGGTCGTTTCTTATTCTTGCTCGAGAGTTTATATCAACAGAGCCAAGC
GCAATTCTCGCAAATTTTGCAGATCCAAGGTCGCGATCGACTCTATTTTGCGCGCTCACGGGAAGAGTTACTCAAGGCCA
GTGCTACCGCCAACCCCAAGGAAATCGGTAATACCGGATTTTGGGTCACGACCAACAATAACACCGCCAAAAAACGGGCG
ATTTTAGTCGAAGCCTTAATGCAATTTGGCTGCGATGCCCAAATCGCCAACGGCATTGCCGAACGCGTATAA

Upstream 100 bases:

>100_bases
ATAGATACACTGGGGCAACGATCGCACAGATCCTTTGCGAGGATCGCCTGTGGTGCACTTTTTCGGTTAAGCCTATCGAA
TAACAAAGGATAATAAGGTT

Downstream 100 bases:

>100_bases
CGCTTTTATCGACGCCGCAATGATGCGGCGTTTCCATTTAATTAACCGATTTTCCAGTAGGAGAGGATCAGGTGGCAATA
CATCAACGGGCAGGACAAAC

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEQAQPQAISQPSLEATTPEPQIAPQIETLVSATDFNQLVD
EHKLEQQKGAVGRFLFLLESLYQQSQAQFSQILQIQGRDRLYFARSREELLKASATANPKEIGNTGFWVTTNNNTAKKRA
ILVEALMQFGCDAQIANGIAERV

Sequences:

>Translated_183_residues
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEQAQPQAISQPSLEATTPEPQIAPQIETLVSATDFNQLVD
EHKLEQQKGAVGRFLFLLESLYQQSQAQFSQILQIQGRDRLYFARSREELLKASATANPKEIGNTGFWVTTNNNTAKKRA
ILVEALMQFGCDAQIANGIAERV
>Mature_183_residues
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEQAQPQAISQPSLEATTPEPQIAPQIETLVSATDFNQLVD
EHKLEQQKGAVGRFLFLLESLYQQSQAQFSQILQIQGRDRLYFARSREELLKASATANPKEIGNTGFWVTTNNNTAKKRA
ILVEALMQFGCDAQIANGIAERV

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form [H]

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=172, Percent_Identity=40.6976744186046, Blast_Score=111, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 20463; Mature: 20463

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEQAQPQAISQPSLEATTPEP
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCC
QIAPQIETLVSATDFNQLVDEHKLEQQKGAVGRFLFLLESLYQQSQAQFSQILQIQGRDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LYFARSREELLKASATANPKEIGNTGFWVTTNNNTAKKRAILVEALMQFGCDAQIANGIA
EEHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ERV
HCC
>Mature Secondary Structure
MKYIEVDEELYRHIASKTERIGESASDILRRLLGLSVDAVEQAQPQAISQPSLEATTPEP
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCC
QIAPQIETLVSATDFNQLVDEHKLEQQKGAVGRFLFLLESLYQQSQAQFSQILQIQGRDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LYFARSREELLKASATANPKEIGNTGFWVTTNNNTAKKRAILVEALMQFGCDAQIANGIA
EEHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ERV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]