Click here to switch to the map view.

The map label for this gene is norM [H]

Identifier: 117920484

GI number: 117920484

Start: 2448169

End: 2449548

Strand: Reverse

Name: norM [H]

Synonym: Shewana3_2039

Alternate gene names: 117920484

Gene position: 2449548-2448169 (Counterclockwise)

Preceding gene: 117920486

Following gene: 117920468

Centisome position: 49.26

GC content: 50.36

Gene sequence:

>1380_bases
ATGAATCATTCAGGCTTTCAGGCCAAACGCTTAATTCAACTCGCACTCCCTGTGTTGATTGCCCAAGTGACCCAAACCAT
GATGGGGTTTATCGATACGGTCATGGCGGGCCGCGTCAGTGCTGTCGATATGGCCGCCGTGGCCGTAGGAACCAGTCTTT
GGTTACCCGCCATCTTATTTGTCCAAGGGTTGCTGATGGCGTTTACGCCGTTATTTGCCCATCATAATGGCGCAAATAAT
CAGAAAGCCATTCAACCGCTGGCGTTTCAAGGCGCTTATCTGGCCTTAATTGGTGGCCTTGGCGTGATGGCGTTTCTTGC
ATCCGCCCCACTCGTGCTCAGCCATATGAATTTAGAGCCCCAATTGTACAACTTAACCATAGGATATATCGACGGTATTT
TATGGGGCGCGCCCGCATTTGTGCTCTATCAGGTATTACGCGGTTGCAGCGAAGGGATTTCTTACACATTACCGACCATG
ATCATCGGATTCGTTGGGCTCGCGGTGAATATTCCGGCGAACTATATCTTTATCTATGGTCATTTCGGCATGCCAGCAAT
GGGCGGCGCCGGCTGTGGTGTCGCGACAGCACTAGTGTTTTGGGCCATGTTGATTGCCATGACGATTTATATGCAGCTAC
ATAAAAAGTTTGCCGCATTGGCGCCCTTTAGCCAGTTCCATCGCCCTGATTTTGCCACCATGAAGAAGATGACTAAGCTT
GGAATGCCGATTGCGATGGCGCTCTTCTTTGAGGTGAGCCTATTTGCAATTATTGCCCTGCTGCTGGCGCCATTGGGCGC
GACTGTCGTGGCCAGTCATCAGATTGCGCTGAACTTCTCCGCCATAGTATTTATGTTGCCACTCTCGATTGGTATTGCCG
TCTCGATTCGTATTGGCTACTACCTCGGCCGCGATCGCGCGGATATTTCTGCCGTGGTGGCAAAGGTTGGATTGTGGTTG
GCGCTTTCATTGGCCTTGTCGACGGCAATCTTAACGGTGTTATTCCGCTTCCAAATCGCTGAGTTGTATAACAGCGATCC
TGAAGTGGTGGTACTTGCCGGCAGTTTGATGCTCATGGCCGCGCTGTATCAATTATCTGACTCAATCCAAGTGGTCGCTG
CCGGTGCCTTGCGTGGATATAAAGATACCCGCAGTGCGTTTTATATTACGCTGTTTTCCTACTGGGGCATTGGCATGACA
TTAGGTTATACCTTGGCGTATACCGACTTTATCGTACCCGCCATGGGCGCCCATGGTTTTTGGACTGGATTAATTGCCGG
CCTCACCTCAGCGGCGCTGCTGTTTTTCATCCGCCTTCGTTATATTCAAAAGCATGGTGTGCATTTAAGTTTGATTGAAG
GCGACAGCGTGCATCATTAA

Upstream 100 bases:

>100_bases
TATTTAGGCCATCTTTCTTATGTATTGCAACCACCTCACAGGTGGCTTGAACTATACCAGTAAACATTTTTTGCTCGCTT
ATTTGTTAAAGGCTGTATTT

Downstream 100 bases:

>100_bases
GCAGATTGAAAGATAAAACGGCGAGCATTTGGCTCGCCGTTTTATTTCGCGCGGGGTCATTTGGGCCCTCATCACAGTCT
AACTTGCCGCTTTATCAACA

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNHSGFQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILFVQGLLMAFTPLFAHHNGANN
QKAIQPLAFQGAYLALIGGLGVMAFLASAPLVLSHMNLEPQLYNLTIGYIDGILWGAPAFVLYQVLRGCSEGISYTLPTM
IIGFVGLAVNIPANYIFIYGHFGMPAMGGAGCGVATALVFWAMLIAMTIYMQLHKKFAALAPFSQFHRPDFATMKKMTKL
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGYYLGRDRADISAVVAKVGLWL
ALSLALSTAILTVLFRFQIAELYNSDPEVVVLAGSLMLMAALYQLSDSIQVVAAGALRGYKDTRSAFYITLFSYWGIGMT
LGYTLAYTDFIVPAMGAHGFWTGLIAGLTSAALLFFIRLRYIQKHGVHLSLIEGDSVHH

Sequences:

>Translated_459_residues
MNHSGFQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILFVQGLLMAFTPLFAHHNGANN
QKAIQPLAFQGAYLALIGGLGVMAFLASAPLVLSHMNLEPQLYNLTIGYIDGILWGAPAFVLYQVLRGCSEGISYTLPTM
IIGFVGLAVNIPANYIFIYGHFGMPAMGGAGCGVATALVFWAMLIAMTIYMQLHKKFAALAPFSQFHRPDFATMKKMTKL
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGYYLGRDRADISAVVAKVGLWL
ALSLALSTAILTVLFRFQIAELYNSDPEVVVLAGSLMLMAALYQLSDSIQVVAAGALRGYKDTRSAFYITLFSYWGIGMT
LGYTLAYTDFIVPAMGAHGFWTGLIAGLTSAALLFFIRLRYIQKHGVHLSLIEGDSVHH
>Mature_459_residues
MNHSGFQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILFVQGLLMAFTPLFAHHNGANN
QKAIQPLAFQGAYLALIGGLGVMAFLASAPLVLSHMNLEPQLYNLTIGYIDGILWGAPAFVLYQVLRGCSEGISYTLPTM
IIGFVGLAVNIPANYIFIYGHFGMPAMGGAGCGVATALVFWAMLIAMTIYMQLHKKFAALAPFSQFHRPDFATMKKMTKL
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGYYLGRDRADISAVVAKVGLWL
ALSLALSTAILTVLFRFQIAELYNSDPEVVVLAGSLMLMAALYQLSDSIQVVAAGALRGYKDTRSAFYITLFSYWGIGMT
LGYTLAYTDFIVPAMGAHGFWTGLIAGLTSAALLFFIRLRYIQKHGVHLSLIEGDSVHH

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=455, Percent_Identity=23.5164835164835, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI22907060, Length=460, Percent_Identity=22.1739130434783, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI38261960, Length=491, Percent_Identity=22.6069246435845, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI48994890, Length=439, Percent_Identity=52.3917995444191, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI226510954, Length=460, Percent_Identity=22.6086956521739, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320545, Length=356, Percent_Identity=24.438202247191, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321821, Length=395, Percent_Identity=21.5189873417722, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49434; Mature: 49434

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSGFQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VQGLLMAFTPLFAHHNGANNQKAIQPLAFQGAYLALIGGLGVMAFLASAPLVLSHMNLEP
HHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
QLYNLTIGYIDGILWGAPAFVLYQVLRGCSEGISYTLPTMIIGFVGLAVNIPANYIFIYG
EEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCEEEEEE
HFGMPAMGGAGCGVATALVFWAMLIAMTIYMQLHKKFAALAPFSQFHRPDFATMKKMTKL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHH
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCEEEEEEEEE
YLGRDRADISAVVAKVGLWLALSLALSTAILTVLFRFQIAELYNSDPEVVVLAGSLMLMA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
ALYQLSDSIQVVAAGALRGYKDTRSAFYITLFSYWGIGMTLGYTLAYTDFIVPAMGAHGF
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
WTGLIAGLTSAALLFFIRLRYIQKHGVHLSLIEGDSVHH
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MNHSGFQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VQGLLMAFTPLFAHHNGANNQKAIQPLAFQGAYLALIGGLGVMAFLASAPLVLSHMNLEP
HHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
QLYNLTIGYIDGILWGAPAFVLYQVLRGCSEGISYTLPTMIIGFVGLAVNIPANYIFIYG
EEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCEEEEEE
HFGMPAMGGAGCGVATALVFWAMLIAMTIYMQLHKKFAALAPFSQFHRPDFATMKKMTKL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHH
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCEEEEEEEEE
YLGRDRADISAVVAKVGLWLALSLALSTAILTVLFRFQIAELYNSDPEVVVLAGSLMLMA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
ALYQLSDSIQVVAAGALRGYKDTRSAFYITLFSYWGIGMTLGYTLAYTDFIVPAMGAHGF
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
WTGLIAGLTSAALLFFIRLRYIQKHGVHLSLIEGDSVHH
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]