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The map label for this gene is aspS [H]

Identifier: 117920398

GI number: 117920398

Start: 2324112

End: 2325887

Strand: Direct

Name: aspS [H]

Synonym: Shewana3_1953

Alternate gene names: 117920398

Gene position: 2324112-2325887 (Clockwise)

Preceding gene: 117920397

Following gene: 117920399

Centisome position: 46.74

GC content: 48.99

Gene sequence:

>1776_bases
ATGCGCAGTCATTATTGTGGAGACGTCAATAAGTCTCACGTTGGACAAGAAGTTACCTTGGTAGGTTGGGTTAATCGTAG
CCGTGATTTGGGTGGCGTGGTCTTTTTAGATTTAAGAGACAGAGAAGGTCTGGTCCAAGTGGTTTACGATCCAGATTTAC
CTGAGGTGTTCAATGTGGCCAGTACGCTGCGCGCCGAGTTTTGTGTGCAGGTAAAAGGCGTGGTTCGTGCCCGTCCTGAT
AGCCAAGTCAACGCGCAAATGAAGACCGGCGAAATTGAAGTATTAGGTAAAGAACTGACGATCATCAACAGTTCTGAGCC
GCTGCCGCTGAGCTTAGATAACTATCAAAACAACAGTGAAGAGCAGCGTCTAAAGTACCGTTATTTAGATTTACGTCGCC
CAGAAATGGCGCAGCGCTTAATGTTCCGCGCGAAAGTGACCAGTGCCGTGCGTCGTTTCCTCGACTCAAACGGCTTCTTA
GATATCGAAACGCCTATTCTGACCAAGGCGACGCCAGAAGGTGCCCGTGACTATTTAGTGCCAAGCCGCACTTACAAAGG
TCAATTCTTCGCGCTGCCACAATCACCACAGCTGTTTAAGCAGTTGCTGATGATGTCAGGCTTTGACCGTTACTATCAAA
TCGTAAAATGCTTCCGTGACGAAGATTTACGTGCTGACCGTCAGCCAGAATTTACTCAAATCGATATCGAAACCTCATTT
ATGACGTCTGAGCAGGTGATGGCTAAGACCGAAGAGATGATGCGTGGTCTGTTCCTTGAGATGCTGAACGTCGATTTAGG
CGAATTCCCACGTATGACCTATAACGAAGCCATGCGTCGTTTCGGTTCAGATAAGCCAGATTTACGTAACCCATTAGAAC
TGGTTGACGTGGCTGACTTGCTCAAAGAAGTGGAGTTTGCGGTATTCTCAGGCCCAGCTAATGACGAAGAAGGCCGTGTT
GCGGCGCTGCGTATTCCAGGTGGTGCGAGCTTATCCCGTAAGCAAATCGATGATTACACTAAGTTTGTCGGTATTTACGG
TGCTAAAGGTTTAGCTTGGATGAAGCTAAACGACCTGACTCAAGGGTTAGAAGGCATTCAATCACCTGTGCTGAAGTTCT
TGAACGAAGGCATTGTGAACGAAATCATCAGCCGTACTGGCGCACAAACGGGCGATATCATCCTGTTTGGTGCCGATAAC
GCGACTGTGGTTGCTGAATCTATGGGCGCACTGCGTCTTAAAGCCGGTGAAGACTTTAATCTGCTCGAAGGTCAATGGCG
TCCACTGTGGGTGGTTGACTTCCCAATGTTTGAGAAGATCAACGGCAGCTTCCACGCGGTTCACCATCCGTTTACTGCGC
CACGTGGCGTGACACCACAAGAGCTGGAAGCCAATCCGGCTAACCGTGTGTCAGATGCCTACGACATGGTATTAAACGGC
TGTGAATTAGGTGGTGGTTCAGTTCGTATTCACAACCAAGAGATGCAATCTGCGGTATTCCGTATTCTTGGCATTACCGA
TGAAGAAGCGAAAGAGAAATTCGGCTTCTTACTCGAAGCGCTGCGCTACGGTACGCCACCACATGCGGGTCTGGCATTCG
GTTTAGACCGTATTATCATGCTGATGACGGGCGCAAGCTCAATCCGTGATGTGATGGCTTTCCCGAAAACCACCACAGCG
GCGTGTCCGTTAACCAATGCCCCAGGTTTTGCTAACCCACAACAATTAGCTGAACTAGGTATTTCCGTGGTGAAAGCCGC
GAAGACTGAAGACTAA

Upstream 100 bases:

>100_bases
TGAAGTACTTTGGTAATCGGCATTATTTGACTATAATGCCGCTTTTATCTCGTTTTGATTATTTCCAAAGCACCGGTTTC
CGGATAAAGGATAGAGTTAG

Downstream 100 bases:

>100_bases
TCTTTAGTGATTATCGAGTACCGAGGCGCAAGCCTCGGTCTATTTTTACCCCGTCATTTCATTTCTTATCAATATCTGCG
TTATCGACGTCACTGAATTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVASTLRAEFCVQVKGVVRARPD
SQVNAQMKTGEIEVLGKELTIINSSEPLPLSLDNYQNNSEEQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADLLKEVEFAVFSGPANDEEGRV
AALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLTQGLEGIQSPVLKFLNEGIVNEIISRTGAQTGDIILFGADN
ATVVAESMGALRLKAGEDFNLLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGFANPQQLAELGISVVKAAKTED

Sequences:

>Translated_591_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVASTLRAEFCVQVKGVVRARPD
SQVNAQMKTGEIEVLGKELTIINSSEPLPLSLDNYQNNSEEQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADLLKEVEFAVFSGPANDEEGRV
AALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLTQGLEGIQSPVLKFLNEGIVNEIISRTGAQTGDIILFGADN
ATVVAESMGALRLKAGEDFNLLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGFANPQQLAELGISVVKAAKTED
>Mature_591_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVASTLRAEFCVQVKGVVRARPD
SQVNAQMKTGEIEVLGKELTIINSSEPLPLSLDNYQNNSEEQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADLLKEVEFAVFSGPANDEEGRV
AALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLTQGLEGIQSPVLKFLNEGIVNEIISRTGAQTGDIILFGADN
ATVVAESMGALRLKAGEDFNLLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGFANPQQLAELGISVVKAAKTED

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=39.4389438943894, Blast_Score=411, Evalue=1e-114,
Organism=Homo sapiens, GI5031815, Length=249, Percent_Identity=27.710843373494, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI194272210, Length=249, Percent_Identity=27.710843373494, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI45439306, Length=268, Percent_Identity=28.3582089552239, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI226491502, Length=262, Percent_Identity=27.0992366412214, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4758762, Length=289, Percent_Identity=24.2214532871972, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=68.6971235194585, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=27.9863481228669, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1789256, Length=293, Percent_Identity=27.3037542662116, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=33.7209302325581, Blast_Score=334, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71984122, Length=306, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71994340, Length=180, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=275, Percent_Identity=26.5454545454545, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=180, Percent_Identity=28.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=180, Percent_Identity=28.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=323, Percent_Identity=37.1517027863777, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=266, Percent_Identity=28.1954887218045, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=252, Percent_Identity=26.5873015873016, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=178, Percent_Identity=24.7191011235955, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=263, Percent_Identity=24.3346007604563, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=35.2649006622517, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640849, Length=246, Percent_Identity=27.2357723577236, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24640851, Length=246, Percent_Identity=27.2357723577236, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17136276, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65993; Mature: 65993

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
STLRAEFCVQVKGVVRARPDSQVNAQMKTGEIEVLGKELTIINSSEPLPLSLDNYQNNSE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECEEEEEECCCCCCEEECCCCCCCH
EQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFLDIETPILTKATPEGARDYLV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
MTSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKEVEFAVFSGPANDEEGRVAALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLT
HHHHHHHEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
QGLEGIQSPVLKFLNEGIVNEIISRTGAQTGDIILFGADNATVVAESMGALRLKAGEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCCEEEECCCCCC
LLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHC
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIM
CCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMTGASSIRDVMAFPKTTTAACPLTNAPGFANPQQLAELGISVVKAAKTED
HHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
STLRAEFCVQVKGVVRARPDSQVNAQMKTGEIEVLGKELTIINSSEPLPLSLDNYQNNSE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECEEEEEECCCCCCEEECCCCCCCH
EQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFLDIETPILTKATPEGARDYLV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
MTSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKEVEFAVFSGPANDEEGRVAALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLT
HHHHHHHEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
QGLEGIQSPVLKFLNEGIVNEIISRTGAQTGDIILFGADNATVVAESMGALRLKAGEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCCEEEECCCCCC
LLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHC
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIM
CCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMTGASSIRDVMAFPKTTTAACPLTNAPGFANPQQLAELGISVVKAAKTED
HHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA