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The map label for this gene is dapE [H]

Identifier: 117920357

GI number: 117920357

Start: 2268700

End: 2269845

Strand: Reverse

Name: dapE [H]

Synonym: Shewana3_1912

Alternate gene names: 117920357

Gene position: 2269845-2268700 (Counterclockwise)

Preceding gene: 117920358

Following gene: 117920356

Centisome position: 45.65

GC content: 53.4

Gene sequence:

>1146_bases
ATGACGCACGCTTCGAACACCCACCCAGTTACCGAACTGACTAAAGAATTAATCGCCCGTCCTTCGGTCACCCCGCTGGA
TGAAGGCTGCCAAACCCTGATGGCCGAACGTCTCGCCGCCATTGGCTTTAATATTGAGCCCATGGTCTTTGAAGACACCA
CCAATATGTGGGCGCGTCGCGGTAATGAAGGCCCAGTATTTTGTTTTGCCGGCCATACCGATGTAGTGCCCGCAGGCGAT
TTAAGCCGCTGGCATACCCCGCCTTTTGAGCCAACCATTATCGACGGTTACCTCTATGGGCGCGGCGCAGCCGACATGAA
AGGCTCACTGGCGGCGATGATCGTCGCGACCGAGCGTTTTGTGGCTAAGCACCCAAATCATCCCGGCTCGATTGCGTTTT
TGATCACTAGCGATGAAGAAGGCCCCTTTATCAATGGCACCACACGGGTTATCGACACCCTAGAAGCCCGTAATGAAAAA
ATCACTTGGACGCTGGTGGGCGAACCCTCATCGACCCTAAAACTCGGTGATGTGGTCAAAAATGGGCGCCGTGGCAGCCT
GACCGCCAACTTAACCGTTAAGGGCATTCAGGGCCATGTGGCCTATCCGCACCTTGCGGATAACCCAATCCACAAAGCCG
CGCCGTTTTTAGCCGAGTTAAGCCAAACCCATTGGGATAATGGCAACGAATTCTTCCCGCCCACCAGCATGCAAATCGCC
AATATTAATGGCGGTACGGGCGCATCGAACGTGATCCCAGGCACCCTAGAGGTGATGTTTAACTTCCGTTATTCGACCGA
AGTGACCGCCGAGATATTAATCGAGCGCGTGGAAGCCTTATTAACCGCCCACGAACTGGATTATGACATCAGCTGGACCT
TCAACGGTCTGCCATTTTTAACCGGCGAAGGCCCACTGTTAGACGCGACTCGCCATGCTATACGTCAGATCACTGGCTAT
GATACCGATCCGCAAACCACGGGCGGCACCTCTGATGGACGCTTTATTGCACCAACGGGCGCCAAAGTGTTGGAACTCGG
TCCAGTGAACGCCACTATCCATAAAGTGAACGAGTGCGTGAAAATCGACGATCTCGAGCAGTTAGCCCTGTGCTACGAAG
TGATTTTGGAACAGTTACTGTGTTAA

Upstream 100 bases:

>100_bases
TGCTCACTCACCCGACGCTGATCAAGCGTCCTGTGCTCACCGTGGGTGAACAAGTGTTGGTAGGCTTTAACGAAGCCGAA
TATAAGAAGGTGTTTTCCCT

Downstream 100 bases:

>100_bases
ACGCCACGCCACGCTTAACCAACACCGCCCAGCTTTATGGGATAGAGAGTGGGGTCACAAGCGGTTTAGACACGGGCTTA
CATACCGACTTATCCGCTGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTHASNTHPVTELTKELIARPSVTPLDEGCQTLMAERLAAIGFNIEPMVFEDTTNMWARRGNEGPVFCFAGHTDVVPAGD
LSRWHTPPFEPTIIDGYLYGRGAADMKGSLAAMIVATERFVAKHPNHPGSIAFLITSDEEGPFINGTTRVIDTLEARNEK
ITWTLVGEPSSTLKLGDVVKNGRRGSLTANLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQTHWDNGNEFFPPTSMQIA
NINGGTGASNVIPGTLEVMFNFRYSTEVTAEILIERVEALLTAHELDYDISWTFNGLPFLTGEGPLLDATRHAIRQITGY
DTDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVKIDDLEQLALCYEVILEQLLC

Sequences:

>Translated_381_residues
MTHASNTHPVTELTKELIARPSVTPLDEGCQTLMAERLAAIGFNIEPMVFEDTTNMWARRGNEGPVFCFAGHTDVVPAGD
LSRWHTPPFEPTIIDGYLYGRGAADMKGSLAAMIVATERFVAKHPNHPGSIAFLITSDEEGPFINGTTRVIDTLEARNEK
ITWTLVGEPSSTLKLGDVVKNGRRGSLTANLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQTHWDNGNEFFPPTSMQIA
NINGGTGASNVIPGTLEVMFNFRYSTEVTAEILIERVEALLTAHELDYDISWTFNGLPFLTGEGPLLDATRHAIRQITGY
DTDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVKIDDLEQLALCYEVILEQLLC
>Mature_380_residues
THASNTHPVTELTKELIARPSVTPLDEGCQTLMAERLAAIGFNIEPMVFEDTTNMWARRGNEGPVFCFAGHTDVVPAGDL
SRWHTPPFEPTIIDGYLYGRGAADMKGSLAAMIVATERFVAKHPNHPGSIAFLITSDEEGPFINGTTRVIDTLEARNEKI
TWTLVGEPSSTLKLGDVVKNGRRGSLTANLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQTHWDNGNEFFPPTSMQIAN
INGGTGASNVIPGTLEVMFNFRYSTEVTAEILIERVEALLTAHELDYDISWTFNGLPFLTGEGPLLDATRHAIRQITGYD
TDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECVKIDDLEQLALCYEVILEQLLC

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501901, Length=297, Percent_Identity=23.5690235690236, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788816, Length=373, Percent_Identity=63.8069705093834, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1790395, Length=272, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789236, Length=305, Percent_Identity=22.9508196721311, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17551016, Length=264, Percent_Identity=28.030303030303, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41475; Mature: 41344

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHASNTHPVTELTKELIARPSVTPLDEGCQTLMAERLAAIGFNIEPMVFEDTTNMWARR
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHC
GNEGPVFCFAGHTDVVPAGDLSRWHTPPFEPTIIDGYLYGRGAADMKGSLAAMIVATERF
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECEEECCCCCCCCCCEEEEEEHHHHH
VAKHPNHPGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWTLVGEPSSTLKLGDVVK
HHHCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHH
NGRRGSLTANLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQTHWDNGNEFFPPTSMQIA
CCCCCCEEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCEEEE
NINGGTGASNVIPGTLEVMFNFRYSTEVTAEILIERVEALLTAHELDYDISWTFNGLPFL
ECCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
TGEGPLLDATRHAIRQITGYDTDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHH
KIDDLEQLALCYEVILEQLLC
CCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THASNTHPVTELTKELIARPSVTPLDEGCQTLMAERLAAIGFNIEPMVFEDTTNMWARR
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHC
GNEGPVFCFAGHTDVVPAGDLSRWHTPPFEPTIIDGYLYGRGAADMKGSLAAMIVATERF
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECEEECCCCCCCCCCEEEEEEHHHHH
VAKHPNHPGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWTLVGEPSSTLKLGDVVK
HHHCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHH
NGRRGSLTANLTVKGIQGHVAYPHLADNPIHKAAPFLAELSQTHWDNGNEFFPPTSMQIA
CCCCCCEEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCEEEE
NINGGTGASNVIPGTLEVMFNFRYSTEVTAEILIERVEALLTAHELDYDISWTFNGLPFL
ECCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
TGEGPLLDATRHAIRQITGYDTDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVNECV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHH
KIDDLEQLALCYEVILEQLLC
CCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]