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The map label for this gene is iaaA [H]

Identifier: 117920340

GI number: 117920340

Start: 2248595

End: 2249626

Strand: Direct

Name: iaaA [H]

Synonym: Shewana3_1895

Alternate gene names: 117920340

Gene position: 2248595-2249626 (Clockwise)

Preceding gene: 117920337

Following gene: 117920341

Centisome position: 45.22

GC content: 50.68

Gene sequence:

>1032_bases
ATGAAAATAAAATTAGCCACCTTTAGTTTAATTCTCTCGAGTGCACTTTTTATGTCACAAAATACCCAAGCGAATGAAAA
ACCTTTTGCCATTGCGATCCACGGTGGCGCTGGCACGATTTCTAAGGCCAACCTCACGCCAGAGCTGCGTCAGGCCTACA
AAGACAAGCTCAAAGAAGCCGTCGACAAAGGCTCAAAAGTGCTTGAGCAGGGCGGTGATAGCTTAGTCGCGGTGCAAACC
GCTATCAATGTATTAGAAAACAGCCCGCTGTTTAACGCCGGTGTCGGTTCGGTTTACACCTATGATGGCGGCCACGAGTT
AGATGCGTCGATTATGGATGGTAAAACCATGAATGCGGGCGCCGTAGCGGGTGTGCGCCATATCGCTAATCCCATCGATT
TGGCCTTAGCCGTGATGAATAAGTCCGAGCATGTCATGCTGTCGGGCGCAGGCGCCGAGGAGTTTGCCTTAACCCAAGGT
TTTAAACTGGTACCTAACAGCCATTTCGATAGCGATGCCAGTTATCAGCAATTACTCGATGCCAGACAAAAACTGCAGGC
GGCTGAAAAATCAGATCAAATTGCGGGTATTGAGATGAAAGATCTCGATTATAAATTTGGTACCGTTGGAGCTGTCGCCT
TAGATAAAAACGGCAACTTAGCGGCGGGGACTTCGACTGGCGGTATGACGGCCAAGCGTTTTGGTCGCATTGGAGACTCG
CCCGTGATTGGGGCTGGCACCTATGCCGAAAACGGCGTTTGCGCTGTGTCGGCCACGGGCCACGGCGAGTTTTTTATTCG
CTACCAAGTGGCGGGCGATATCTGCGCTAAGGTGAAATACCAGCAAAAATCCATTATTCAAGCGGCAGATGAAGTGATTA
ATCAACGCCTTATCACCGCTGGCGGCTCTGGTGGTGTGATAGCGGTCGACCACAGGGGCAATATTGCCACGCCATTTAAC
ACCGAAGGCATGTACCGCGCTACCCGTAGTAATGGCGAGCCTGCCCAAGTGATGATTTGGCAGGACCAATAA

Upstream 100 bases:

>100_bases
AGTTCATTGTCGCCAGAATAGCCGCAACAGGCAAGGAGATGATTGAAATTAGTCATTGAAATGGTTACAAAGGACGTTAC
TGTTTAAGGGGATGTAAATA

Downstream 100 bases:

>100_bases
CCCATATCAATTGCCTTAGCGCACGTGAAGTGAGCGCTAAGGCTCATATCCTCAACGCGTTACCATCACAATGGGCGTTT
TAAGATCCAATTTAGGCCCA

Product: peptidase T2, asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKIKLATFSLILSSALFMSQNTQANEKPFAIAIHGGAGTISKANLTPELRQAYKDKLKEAVDKGSKVLEQGGDSLVAVQT
AINVLENSPLFNAGVGSVYTYDGGHELDASIMDGKTMNAGAVAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQG
FKLVPNSHFDSDASYQQLLDARQKLQAAEKSDQIAGIEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDS
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDHRGNIATPFN
TEGMYRATRSNGEPAQVMIWQDQ

Sequences:

>Translated_343_residues
MKIKLATFSLILSSALFMSQNTQANEKPFAIAIHGGAGTISKANLTPELRQAYKDKLKEAVDKGSKVLEQGGDSLVAVQT
AINVLENSPLFNAGVGSVYTYDGGHELDASIMDGKTMNAGAVAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQG
FKLVPNSHFDSDASYQQLLDARQKLQAAEKSDQIAGIEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDS
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDHRGNIATPFN
TEGMYRATRSNGEPAQVMIWQDQ
>Mature_343_residues
MKIKLATFSLILSSALFMSQNTQANEKPFAIAIHGGAGTISKANLTPELRQAYKDKLKEAVDKGSKVLEQGGDSLVAVQT
AINVLENSPLFNAGVGSVYTYDGGHELDASIMDGKTMNAGAVAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQG
FKLVPNSHFDSDASYQQLLDARQKLQAAEKSDQIAGIEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDS
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITAGGSGGVIAVDHRGNIATPFN
TEGMYRATRSNGEPAQVMIWQDQ

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=306, Percent_Identity=38.8888888888889, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI145275200, Length=306, Percent_Identity=38.8888888888889, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI98991769, Length=335, Percent_Identity=30.1492537313433, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI285002251, Length=237, Percent_Identity=32.4894514767932, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1787050, Length=313, Percent_Identity=50.1597444089457, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI32564649, Length=302, Percent_Identity=26.8211920529801, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18921183, Length=305, Percent_Identity=37.0491803278689, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=34.2007434944238, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI221330102, Length=232, Percent_Identity=32.7586206896552, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652135, Length=232, Percent_Identity=32.7586206896552, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 36162; Mature: 36162

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLATFSLILSSALFMSQNTQANEKPFAIAIHGGAGTISKANLTPELRQAYKDKLKEA
CEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
VDKGSKVLEQGGDSLVAVQTAINVLENSPLFNAGVGSVYTYDGGHELDASIMDGKTMNAG
HHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCEEECCCCCCCH
AVAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQGFKLVPNSHFDSDASYQQLLD
HHHHHHHHCCHHHHHEEEECCCCEEEEECCCCHHHHHHCCCEECCCCCCCCCCHHHHHHH
ARQKLQAAEKSDQIAGIEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDS
HHHHHHHHHCCCCCCCEEECCCCEECCCEEEEEECCCCCEEECCCCCCCCHHHHCCCCCC
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITA
CEEECCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEE
GGSGGVIAVDHRGNIATPFNTEGMYRATRSNGEPAQVMIWQDQ
CCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MKIKLATFSLILSSALFMSQNTQANEKPFAIAIHGGAGTISKANLTPELRQAYKDKLKEA
CEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
VDKGSKVLEQGGDSLVAVQTAINVLENSPLFNAGVGSVYTYDGGHELDASIMDGKTMNAG
HHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCEEECCCCCCCH
AVAGVRHIANPIDLALAVMNKSEHVMLSGAGAEEFALTQGFKLVPNSHFDSDASYQQLLD
HHHHHHHHCCHHHHHEEEECCCCEEEEECCCCHHHHHHCCCEECCCCCCCCCCHHHHHHH
ARQKLQAAEKSDQIAGIEMKDLDYKFGTVGAVALDKNGNLAAGTSTGGMTAKRFGRIGDS
HHHHHHHHHCCCCCCCEEECCCCEECCCEEEEEECCCCCEEECCCCCCCCHHHHCCCCCC
PVIGAGTYAENGVCAVSATGHGEFFIRYQVAGDICAKVKYQQKSIIQAADEVINQRLITA
CEEECCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEE
GGSGGVIAVDHRGNIATPFNTEGMYRATRSNGEPAQVMIWQDQ
CCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]