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The map label for this gene is aat [H]

Identifier: 117920203

GI number: 117920203

Start: 2070388

End: 2071098

Strand: Reverse

Name: aat [H]

Synonym: Shewana3_1756

Alternate gene names: 117920203

Gene position: 2071098-2070388 (Counterclockwise)

Preceding gene: 117920206

Following gene: 117920202

Centisome position: 41.65

GC content: 48.52

Gene sequence:

>711_bases
GTGAACTCACTGTCTTTTCTGAATCACGAATTTGAAGCTTTTCCTTCGCCCGAACTCGCTCTAACTGATCCAAACGGTTT
ATTGGCGATAGGCGGCGATCTTCGCCCTGAACGTTTATTGACCGCATATTATCATGGCATTTTCCCTTGGTTTAACGCCG
ACGATCCCATTTTGTGGTGGTCACCGGATCCACGCGCCATTTTTATTCCTGGCCAAGTGAATATCAGCACCAGTTTGCGT
AAATATTTAAAAAAACAGCCCTGGCGTTTCACCATCAACCATGCCTTTACCGATGTCATGGCGGGCTGCGCTCAACCTCG
GCGTAAGCAAGCCGGCACTTGGATAACCCATGAAATTCAAATGGCTTATCGCGAGTTGCACCATAATGGCCATGCCCATT
CCGTCGAAGTCTGGCACGGCGAGCGTTTAATTGGCGGACTTTATGGCATTGCCATCGGCCAAGTCTTTTGTGGGGAATCC
ATGTTCCACCGCGAAACTAACGCATCCAAGGCGGCCATGGCGGTGTTGCAACAGCATCTTATCAAAATGAATTTTAAACT
GATTGACGCACAAGTGATGAATCCCCACCTTGAGAGCTTAGGGGCCAAACCGGTGAAGCGCGCTGATTTTATTCAACTCT
TAACTCAGTTTAGGGATAAGCCAGTCAACCCTGCGGCTTGGATACCAAGTGAGGTCACACTTGAACTCTAA

Upstream 100 bases:

>100_bases
CTAAAGCACAAATAAACATAAAGCTCACTAGTGTAGGTTTCCACATATCCGAATTTCCGCTAACTTAATATAAGGGTCTA
TTACTGAATCATACGCAATT

Downstream 100 bases:

>100_bases
CGCAAGTAACACGCCCATCGCTATTGGGATCAGCCAAATCTTTCCCTGCAGTTATCTGGATGGACAGCAAGAACAACTGT
TGGTGATCCAAGAGGAAACA

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNSLSFLNHEFEAFPSPELALTDPNGLLAIGGDLRPERLLTAYYHGIFPWFNADDPILWWSPDPRAIFIPGQVNISTSLR
KYLKKQPWRFTINHAFTDVMAGCAQPRRKQAGTWITHEIQMAYRELHHNGHAHSVEVWHGERLIGGLYGIAIGQVFCGES
MFHRETNASKAAMAVLQQHLIKMNFKLIDAQVMNPHLESLGAKPVKRADFIQLLTQFRDKPVNPAAWIPSEVTLEL

Sequences:

>Translated_236_residues
MNSLSFLNHEFEAFPSPELALTDPNGLLAIGGDLRPERLLTAYYHGIFPWFNADDPILWWSPDPRAIFIPGQVNISTSLR
KYLKKQPWRFTINHAFTDVMAGCAQPRRKQAGTWITHEIQMAYRELHHNGHAHSVEVWHGERLIGGLYGIAIGQVFCGES
MFHRETNASKAAMAVLQQHLIKMNFKLIDAQVMNPHLESLGAKPVKRADFIQLLTQFRDKPVNPAAWIPSEVTLEL
>Mature_236_residues
MNSLSFLNHEFEAFPSPELALTDPNGLLAIGGDLRPERLLTAYYHGIFPWFNADDPILWWSPDPRAIFIPGQVNISTSLR
KYLKKQPWRFTINHAFTDVMAGCAQPRRKQAGTWITHEIQMAYRELHHNGHAHSVEVWHGERLIGGLYGIAIGQVFCGES
MFHRETNASKAAMAVLQQHLIKMNFKLIDAQVMNPHLESLGAKPVKRADFIQLLTQFRDKPVNPAAWIPSEVTLEL

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine [H]

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family [H]

Homologues:

Organism=Escherichia coli, GI1787111, Length=217, Percent_Identity=53.4562211981567, Blast_Score=243, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR004616 [H]

Pfam domain/function: PF03588 Leu_Phe_trans [H]

EC number: =2.3.2.6 [H]

Molecular weight: Translated: 26816; Mature: 26816

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLSFLNHEFEAFPSPELALTDPNGLLAIGGDLRPERLLTAYYHGIFPWFNADDPILWW
CCCCHHHCCHHHCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
SPDPRAIFIPGQVNISTSLRKYLKKQPWRFTINHAFTDVMAGCAQPRRKQAGTWITHEIQ
CCCCCEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHH
MAYRELHHNGHAHSVEVWHGERLIGGLYGIAIGQVFCGESMFHRETNASKAAMAVLQQHL
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHH
IKMNFKLIDAQVMNPHLESLGAKPVKRADFIQLLTQFRDKPVNPAAWIPSEVTLEL
HHHHHHEEEHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEC
>Mature Secondary Structure
MNSLSFLNHEFEAFPSPELALTDPNGLLAIGGDLRPERLLTAYYHGIFPWFNADDPILWW
CCCCHHHCCHHHCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
SPDPRAIFIPGQVNISTSLRKYLKKQPWRFTINHAFTDVMAGCAQPRRKQAGTWITHEIQ
CCCCCEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHH
MAYRELHHNGHAHSVEVWHGERLIGGLYGIAIGQVFCGESMFHRETNASKAAMAVLQQHL
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHH
IKMNFKLIDAQVMNPHLESLGAKPVKRADFIQLLTQFRDKPVNPAAWIPSEVTLEL
HHHHHHEEEHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]