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The map label for this gene is ldh [H]

Identifier: 117920189

GI number: 117920189

Start: 2055662

End: 2056696

Strand: Reverse

Name: ldh [H]

Synonym: Shewana3_1742

Alternate gene names: 117920189

Gene position: 2056696-2055662 (Counterclockwise)

Preceding gene: 117920191

Following gene: 117920188

Centisome position: 41.36

GC content: 48.02

Gene sequence:

>1035_bases
GTGGCTGTATTTAATCATGTATCCTTTGATGAACATGAACAGGTCGTATTCTGTCATGATAAAGAGAGTGGCTTAAAAGC
CATCGTTGCCATCCATAACACCAACTTAGGTCCTGCTGTGGGTGGCTGTCGGATGTGGAACTACCAATCCGACGATGAAG
CCCTGACAGACGTATTACGCCTCTCCCGCGGTATGACGTACAAAAACGCCCTCGCAGGTTTGACTATGGGTGGTGGTAAG
TCAGTAATTATTGCCGATCCTAAGCGCCCTGATCGCGAAGCCCTCTTCCGTGCTTTTGGCCGTTTTATCAATAGCCTGGG
TGGACGTTACTATTCAGCAGAAGACGTAGGTACCACTACAGCGGATATCATGATCGCCCATCAAGAAACCCCATACATGG
CGGGTCTTGAAGGCAAGAGCGGCGATCCATCACCCTTTACTGCATTAGGCACCTATTTAGGGATCAAAGCAGCCGTTAAA
CACAAACTCGGTTTAGACAGCTTAAAAGGCCTTAAAATTGCCGTTCAAGGTGTGGGCCATGTAGGTTACTACCTGTGTAA
ACACCTGCATGAAGAAGGTGCAGAGCTTATCGTCACCGATATTCACCAAGCTTCACTCGACAAAGTGGCTACCGACTTTG
GCGCCATCGTCGTTGCCCCACAGGATATCTATGCCCAAGACGTTGATGTCTATGCGCCATGTGCATTAGGTGCCACCTTA
AACGATGTGACCTTGCCACAGCTTAAAGCTAAGATTGTTGCCGGTTGCGCCAACAACCAATTAGCCGAAGTTCGCCATGG
CGAGCAGTTAAAAGAAATGGGCATTCTGTATGCACCAGACTATGTGATTAACGCTGGCGGTATCATCAACGTGTCATTTG
AAAAAGACTATGACGCGGCGAAATCAGAAGCTAAGGTCAGAGAAATCTACAATACATTGCTGAAGATTTTCACTAAAGCC
GATGCTGAAAACCGCACTACTGCGGCGGTTGCAGACGAAATGGCCCGTGCCATTTATCAAGCGGCAAAAGCTTAA

Upstream 100 bases:

>100_bases
GAGAGTATCGCTAAATGCTATGGTTCTGCAATAAATATAACGAGCTGTCGATGGGAAGTGATTCCATCGACGTTTTATTG
TGTATAGCGGAGAACTTCAC

Downstream 100 bases:

>100_bases
CACTGCTTTCGGCGGACAACGACTTGTTGTCCGCCTCAACCTCTTCCCTCGCTTCGCTTATCCAGCTAAAAAATAAATTC
CTCTTAATACCAATCAGATA

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLRLSRGMTYKNALAGLTMGGGK
SVIIADPKRPDREALFRAFGRFINSLGGRYYSAEDVGTTTADIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVK
HKLGLDSLKGLKIAVQGVGHVGYYLCKHLHEEGAELIVTDIHQASLDKVATDFGAIVVAPQDIYAQDVDVYAPCALGATL
NDVTLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAAKSEAKVREIYNTLLKIFTKA
DAENRTTAAVADEMARAIYQAAKA

Sequences:

>Translated_344_residues
MAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLRLSRGMTYKNALAGLTMGGGK
SVIIADPKRPDREALFRAFGRFINSLGGRYYSAEDVGTTTADIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVK
HKLGLDSLKGLKIAVQGVGHVGYYLCKHLHEEGAELIVTDIHQASLDKVATDFGAIVVAPQDIYAQDVDVYAPCALGATL
NDVTLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAAKSEAKVREIYNTLLKIFTKA
DAENRTTAAVADEMARAIYQAAKA
>Mature_343_residues
AVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLRLSRGMTYKNALAGLTMGGGKS
VIIADPKRPDREALFRAFGRFINSLGGRYYSAEDVGTTTADIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKH
KLGLDSLKGLKIAVQGVGHVGYYLCKHLHEEGAELIVTDIHQASLDKVATDFGAIVVAPQDIYAQDVDVYAPCALGATLN
DVTLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAAKSEAKVREIYNTLLKIFTKAD
AENRTTAAVADEMARAIYQAAKA

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=314, Percent_Identity=24.8407643312102, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4885281, Length=314, Percent_Identity=24.8407643312102, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 37082; Mature: 36950

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLR
CCEECCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHH
LSRGMTYKNALAGLTMGGGKSVIIADPKRPDREALFRAFGRFINSLGGRYYSAEDVGTTT
HHCCCCHHHHHHCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCE
ADIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKHKLGLDSLKGLKIAVQGVGH
EEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHH
VGYYLCKHLHEEGAELIVTDIHQASLDKVATDFGAIVVAPQDIYAQDVDVYAPCALGATL
HHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEECCCHHHHCCCCEEECCCCCCCC
NDVTLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEECCCCCHH
KSEAKVREIYNTLLKIFTKADAENRTTAAVADEMARAIYQAAKA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVFNHVSFDEHEQVVFCHDKESGLKAIVAIHNTNLGPAVGGCRMWNYQSDDEALTDVLR
CEECCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHH
LSRGMTYKNALAGLTMGGGKSVIIADPKRPDREALFRAFGRFINSLGGRYYSAEDVGTTT
HHCCCCHHHHHHCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCE
ADIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKHKLGLDSLKGLKIAVQGVGH
EEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHH
VGYYLCKHLHEEGAELIVTDIHQASLDKVATDFGAIVVAPQDIYAQDVDVYAPCALGATL
HHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEECCCHHHHCCCCEEECCCCCCCC
NDVTLPQLKAKIVAGCANNQLAEVRHGEQLKEMGILYAPDYVINAGGIINVSFEKDYDAA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEECCCCCHH
KSEAKVREIYNTLLKIFTKADAENRTTAAVADEMARAIYQAAKA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]