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The map label for this gene is yhaR [H]

Identifier: 117920117

GI number: 117920117

Start: 1962045

End: 1962905

Strand: Reverse

Name: yhaR [H]

Synonym: Shewana3_1670

Alternate gene names: 117920117

Gene position: 1962905-1962045 (Counterclockwise)

Preceding gene: 117920118

Following gene: 117920116

Centisome position: 39.48

GC content: 51.57

Gene sequence:

>861_bases
ATGACGGGAATAATAATGACTGACACACAACACCAGAACACTAGCACTCAATCCTTAGCTAATTTGCAGCACGTCAGCTA
TGCGCTGAATAACGGCGTGGGCGAGTTAATCCTTAACCGCGCAGAAGTGCACAACGCCTTCGATGAGGTGATGATCAGCG
AAATGATCGCCGTCCTCGGTTATTTTGCCGAGCACAAAGACTGCAAACTGTTGGTATTAAAAGCCAATGGCAAAAACTTC
AGCGCTGGCGCCGACTTAAACTGGATGCGCAAACAAGCCAAGATGGATTTTGAGCAAAATCTGAATGACGCCAAGGCGCT
GGCTAAGCTGATGCAGGATTTAGATACCTTTCCAAAACCCACTATCGCCTTAGTCCAAGGTGCGGCCTTTGGCGGCGCGC
TTGGGTTAATCTGCGCCAGTGATATTGCCATCGCCACCGAGCGTGCCAGTTTCTGCTTAAGCGAAGTCAAACTAGGGCTG
ATCCCTGCGGTGATTAGCCCCTACGTTGCGCGCGCCATGGGCAATCGCGCCTCACGCCGCTATATGCTGACCGCGGAGCG
TTTTGATGCGCAAACGGCGCTCAAACTCAATGTTATCCATGAGATTAACGATGATTTAGCGGCCGCAGCCCAACCCATTA
TCACCGCGCTACAGGCCAACAGTCCGCAGGGAATGGCATGGGTAAAGACCTTACTTGCCCGCCTCGAAGATGGCGTGATA
GACCAAGATACGATTGATTACACCAGCGAGCGCATCGCCCGCATTCGGGTATCTGATGAAGGCCAAGAAGGTTTAAACGC
CTTCTTTGAAAAACGTCAGCCGAACTGGCACACGCCAACAGATACTCAAGGAGTTCTTTGA

Upstream 100 bases:

>100_bases
CAGCCGCATTAAATGCCCCTATCGAAGACACCCGCTTCGGCGTCTTCCGTATGTAACGCATCTTGCGATGCCTTACGTAT
CACTCATAGAGGGAATCACT

Downstream 100 bases:

>100_bases
TGTTGACTAAGCTTTTAATTGCTAACCGTGGTGAAATTGCCTGCCGCATTATCAAAACCGCGCAGGCCATGGGCGTTCGC
ACCGTAGCCTTATATTCCGA

Product: methylglutaconyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTGIIMTDTQHQNTSTQSLANLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIAVLGYFAEHKDCKLLVLKANGKNF
SAGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALVQGAAFGGALGLICASDIAIATERASFCLSEVKLGL
IPAVISPYVARAMGNRASRRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALQANSPQGMAWVKTLLARLEDGVI
DQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGVL

Sequences:

>Translated_286_residues
MTGIIMTDTQHQNTSTQSLANLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIAVLGYFAEHKDCKLLVLKANGKNF
SAGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALVQGAAFGGALGLICASDIAIATERASFCLSEVKLGL
IPAVISPYVARAMGNRASRRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALQANSPQGMAWVKTLLARLEDGVI
DQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGVL
>Mature_285_residues
TGIIMTDTQHQNTSTQSLANLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIAVLGYFAEHKDCKLLVLKANGKNFS
AGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALVQGAAFGGALGLICASDIAIATERASFCLSEVKLGLI
PAVISPYVARAMGNRASRRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALQANSPQGMAWVKTLLARLEDGVID
QDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGVL

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=270, Percent_Identity=24.4444444444444, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI194097323, Length=271, Percent_Identity=30.2583025830258, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=178, Percent_Identity=25.8426966292135, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=27.9569892473118, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI213417737, Length=178, Percent_Identity=25.8426966292135, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=28.1632653061224, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=26.9961977186312, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=25.9842519685039, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=26.615969581749, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787660, Length=272, Percent_Identity=30.5147058823529, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=27.4285714285714, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=265, Percent_Identity=27.1698113207547, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=29.9065420560748, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=25.3968253968254, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=25.4098360655738, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=29.3838862559242, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=28.4444444444444, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=27.7551020408163, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=27.7551020408163, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=23.1075697211155, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 31334; Mature: 31203

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIIMTDTQHQNTSTQSLANLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIAVLG
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFAEHKDCKLLVLKANGKNFSAGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKP
HHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCC
TIALVQGAAFGGALGLICASDIAIATERASFCLSEVKLGLIPAVISPYVARAMGNRASRR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCE
YMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALQANSPQGMAWVKTLLARLEDGVI
EEEEHHHHCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
DQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGVL
CCCHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGIIMTDTQHQNTSTQSLANLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIAVLG
CCEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFAEHKDCKLLVLKANGKNFSAGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKP
HHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCC
TIALVQGAAFGGALGLICASDIAIATERASFCLSEVKLGLIPAVISPYVARAMGNRASRR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCE
YMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALQANSPQGMAWVKTLLARLEDGVI
EEEEHHHHCCCHHHEEHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
DQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGVL
CCCHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]