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The map label for this gene is cysK [H]

Identifier: 117920009

GI number: 117920009

Start: 1823283

End: 1824251

Strand: Reverse

Name: cysK [H]

Synonym: Shewana3_1561

Alternate gene names: 117920009

Gene position: 1824251-1823283 (Counterclockwise)

Preceding gene: 117920013

Following gene: 117920008

Centisome position: 36.69

GC content: 47.47

Gene sequence:

>969_bases
ATGAGCAAAATTTTCGAAGACAATTCATATACTATTGGCAATACTCCGTTAGTGCGTTTAAACCGCGTAAGCCAAGGTAA
AGTGCTGGCAAAGGTCGAAGCACGTAATCCAAGTTTCAGTGTCAAATGCCGTATCGGTGCCAATATGATTTGGGATGCCG
AGAAAAAAGGCCTGCTCACCAAAGATCACGAACTGATTGAGCCTACCTCTGGTAATACAGGGATTGCCTTAGCCTATGTG
GCGGCCGCCCGTGGTTATAAACTGACGTTAACTATGCCAAATACTATGAGCTTAGAGCGTCGTAAACTGCTGAAAGCCTT
AGGTGCAAACCTAGTGCTGACCGAAGGCGCAAAAGGCATGAAAGGTGCTATCGATAAAGCGGAAGAAATCCGTCAATCGG
CGCCTGAAAAATACATTATGCTGCAGCAGTTTAATAACCCAGCAAACCCTGAAATCCATGAAAAAACCACTGGTCCAGAG
ATTTGGAACGATACCGATGGTGCAGTGGATGTGTTTGTTGCAGGCGTGGGTACTGGCGGTACTATCACAGGTGTAAGCCG
TTACCTGAAGAAAGTCGCTGGCAAAGCCATTACTTCTGTCGCGGTAGAACCTGCTGATTCACCAGTTATCACCCAAACCT
TAGCGGGATTACCAGTGCAACCTGGTCCACACAAAATTCAAGGTATTGGCGCAGGTTTTATCCCTGGTAACTTAGATCTT
GAGTTAATCGATCGCGTTGAAACCGTCACCAACGATGAAGCCATCGACATGGCGCGCCGCTTAATGCAAGAAGAAGGCAT
TTTAGTCGGTATTTCTTCTGGCGCTGCTGTGGTTGCGGCGAACCGCATCGCGGCTCTGCCTGAATTTGCGGATAAAAACA
TTGTGGTTATCCTGCCTTCGGCTGCCGAGCGTTACTTATCTTCTGTACTGTTCCAAGGCCAGTTTGGCGACGAAGAGAAC
ATTCAGTAA

Upstream 100 bases:

>100_bases
CCTTTAATAACCAAATCGAATAAAGAAGAACAAACAATCACTTCTAAATGCGCCACAGGCGATTATGATTCAATAGTGCA
AACATAGGAGCAAGCAATCC

Downstream 100 bases:

>100_bases
TAAGTCGATTCGTCCGGCTAAAAATACCCAGCATTGTCTGGGTATTTTTATGGCCGAAATTGATGGGCAAACACGATTAA
CACCACTTCCCTCTCCTCCA

Product: cysteine synthase

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIFEDNSYTIGNTPLVRLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLTKDHELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIDKAEEIRQSAPEKYIMLQQFNNPANPEIHEKTTGPE
IWNDTDGAVDVFVAGVGTGGTITGVSRYLKKVAGKAITSVAVEPADSPVITQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
ELIDRVETVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPSAAERYLSSVLFQGQFGDEEN
IQ

Sequences:

>Translated_322_residues
MSKIFEDNSYTIGNTPLVRLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLTKDHELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIDKAEEIRQSAPEKYIMLQQFNNPANPEIHEKTTGPE
IWNDTDGAVDVFVAGVGTGGTITGVSRYLKKVAGKAITSVAVEPADSPVITQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
ELIDRVETVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPSAAERYLSSVLFQGQFGDEEN
IQ
>Mature_321_residues
SKIFEDNSYTIGNTPLVRLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLTKDHELIEPTSGNTGIALAYVA
AARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIDKAEEIRQSAPEKYIMLQQFNNPANPEIHEKTTGPEI
WNDTDGAVDVFVAGVGTGGTITGVSRYLKKVAGKAITSVAVEPADSPVITQTLAGLPVQPGPHKIQGIGAGFIPGNLDLE
LIDRVETVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPSAAERYLSSVLFQGQFGDEENI
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=340, Percent_Identity=37.9411764705882, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI295821200, Length=340, Percent_Identity=37.9411764705882, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4557415, Length=340, Percent_Identity=37.9411764705882, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1788754, Length=320, Percent_Identity=76.25, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI2367138, Length=316, Percent_Identity=42.7215189873418, Blast_Score=212, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=53.6184210526316, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=54.140127388535, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17562970, Length=313, Percent_Identity=49.2012779552716, Blast_Score=273, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=50.1628664495114, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17561720, Length=312, Percent_Identity=48.3974358974359, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=50, Blast_Score=200, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=40.3785488958991, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=318, Percent_Identity=37.1069182389937, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17561716, Length=125, Percent_Identity=49.6, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321594, Length=325, Percent_Identity=36.3076923076923, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6321449, Length=334, Percent_Identity=33.2335329341317, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24643623, Length=308, Percent_Identity=37.6623376623377, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI20129101, Length=308, Percent_Identity=37.6623376623377, Blast_Score=150, Evalue=8e-37,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34436; Mature: 34305

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFEDNSYTIGNTPLVRLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLT
CCCEECCCCEEECCCCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
KDHELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGM
CCCHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIDKAEEIRQSAPEKYIMLQQFNNPANPEIHEKTTGPEIWNDTDGAVDVFVAGVGTGG
HHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYLKKVAGKAITSVAVEPADSPVITQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
ELIDRVETVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPS
HHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHCCHHCCCCEEEEECC
AAERYLSSVLFQGQFGDEENIQ
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKIFEDNSYTIGNTPLVRLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLT
CCEECCCCEEECCCCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
KDHELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGM
CCCHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIDKAEEIRQSAPEKYIMLQQFNNPANPEIHEKTTGPEIWNDTDGAVDVFVAGVGTGG
HHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYLKKVAGKAITSVAVEPADSPVITQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
ELIDRVETVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPS
HHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHCCHHCCCCEEEEECC
AAERYLSSVLFQGQFGDEENIQ
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]