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The map label for this gene is xthA [H]

Identifier: 117919951

GI number: 117919951

Start: 1750675

End: 1751487

Strand: Reverse

Name: xthA [H]

Synonym: Shewana3_1503

Alternate gene names: 117919951

Gene position: 1751487-1750675 (Counterclockwise)

Preceding gene: 117919952

Following gene: 117919940

Centisome position: 35.23

GC content: 50.68

Gene sequence:

>813_bases
ATGAAAATCGTTTCTTTCAATATCAATGGATTGCGTTCTCGCCTGCATCAATTGCAAGCGTTAATCGACAGCCATCAGCC
CGACATTATCGGCCTGCAAGAAACCAAAGTGCACGATGAAGCCTTCCCGCTCGCCGAAGTCGAGGCCATGGGCTATCACG
TGCATTACCACGGTGGCAAGGCGCACTATGGCGTAGCCATGCTCTCCAAAGTCGCCCCACTTAAGGTGCAAAAGGGTTTT
GCAACTGATGAAGAAGATGCGCAGCGCCGCATGATTATCGGTACCTTTGCTCAGGCAAATGGCCACCCGCTAACTGTGTT
GAATGGTTATTTTCCTCAGGGCGAAAGCATCGACCATCCGACAAAGTACCCTGCTAAGCGCAAGTTTTATCAGGATTTAA
TGGCGCATTTGCACGCTAATCATAGCAACGATGAAGATATCGCCATCATTGGGGATATCAATATTTCGCCCATCGATTTG
GATATCGGCATCGGTGAAGTTAACCGCAAACGTTGGCTAAAAACCGGCAAATGCAGCTTCCAACCCGAAGAGCGTGAATG
GCTCAAGACCCTGCAAGGTTGGGGATTGGTGGATACCTTCCGTCAACTGCACCCCGACCGCTGCGAGCGTTATTCATGGT
TCGATTACCGCAGCAAAGGCTTCGATGATAACCGTGGCCTACGCATCGATGTGATCTTAGCGACGCCGTCACTGGCCGCA
CGTTTAGTTGAATCCGACGTCGATTACGAGCTGCGCGGTATCGACAAACCCTCAGATCACGCGCCAATTTGGAGCACCTT
TAAAGAAGCCTAA

Upstream 100 bases:

>100_bases
CGTTAGCCACGGCTCAAGCCTGGGCAGATGCTGATCCTTACTTCGCGGCGGGCGTATATCAAAGCGTTGTGGTTAAACCC
TTTAAACGAGTTCTCCCTTA

Downstream 100 bases:

>100_bases
CTCGATTGAGTGACTACTTTAATCGATATAAAAAACATCGGCCTAGGCCGATGTTTTTTTGTTATAGAGTCCAATAAAGT
ACCCATCTAATGTCACTGCC

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKIVSFNINGLRSRLHQLQALIDSHQPDIIGLQETKVHDEAFPLAEVEAMGYHVHYHGGKAHYGVAMLSKVAPLKVQKGF
ATDEEDAQRRMIIGTFAQANGHPLTVLNGYFPQGESIDHPTKYPAKRKFYQDLMAHLHANHSNDEDIAIIGDINISPIDL
DIGIGEVNRKRWLKTGKCSFQPEEREWLKTLQGWGLVDTFRQLHPDRCERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
RLVESDVDYELRGIDKPSDHAPIWSTFKEA

Sequences:

>Translated_270_residues
MKIVSFNINGLRSRLHQLQALIDSHQPDIIGLQETKVHDEAFPLAEVEAMGYHVHYHGGKAHYGVAMLSKVAPLKVQKGF
ATDEEDAQRRMIIGTFAQANGHPLTVLNGYFPQGESIDHPTKYPAKRKFYQDLMAHLHANHSNDEDIAIIGDINISPIDL
DIGIGEVNRKRWLKTGKCSFQPEEREWLKTLQGWGLVDTFRQLHPDRCERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
RLVESDVDYELRGIDKPSDHAPIWSTFKEA
>Mature_270_residues
MKIVSFNINGLRSRLHQLQALIDSHQPDIIGLQETKVHDEAFPLAEVEAMGYHVHYHGGKAHYGVAMLSKVAPLKVQKGF
ATDEEDAQRRMIIGTFAQANGHPLTVLNGYFPQGESIDHPTKYPAKRKFYQDLMAHLHANHSNDEDIAIIGDINISPIDL
DIGIGEVNRKRWLKTGKCSFQPEEREWLKTLQGWGLVDTFRQLHPDRCERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
RLVESDVDYELRGIDKPSDHAPIWSTFKEA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=274, Percent_Identity=27.3722627737226, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI18375503, Length=274, Percent_Identity=27.3722627737226, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI18375501, Length=274, Percent_Identity=27.3722627737226, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=62.6865671641791, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=27.6752767527675, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=34.1818181818182, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17136678, Length=271, Percent_Identity=34.3173431734317, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30741; Mature: 30741

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSFNINGLRSRLHQLQALIDSHQPDIIGLQETKVHDEAFPLAEVEAMGYHVHYHGGK
CEEEEECHHHHHHHHHHHHHHHHCCCCCEECCHHCCCCCHHCCHHHHHHEEEEEEEECCC
AHYGVAMLSKVAPLKVQKGFATDEEDAQRRMIIGTFAQANGHPLTVLNGYFPQGESIDHP
HHHHHHHHHHCCCEEEECCCCCCHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKYPAKRKFYQDLMAHLHANHSNDEDIAIIGDINISPIDLDIGIGEVNRKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCC
QPEEREWLKTLQGWGLVDTFRQLHPDRCERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
CCHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCCHHCCCCCCCCCCEEEEEEECHHHHH
RLVESDVDYELRGIDKPSDHAPIWSTFKEA
HHHHCCCCEEEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKIVSFNINGLRSRLHQLQALIDSHQPDIIGLQETKVHDEAFPLAEVEAMGYHVHYHGGK
CEEEEECHHHHHHHHHHHHHHHHCCCCCEECCHHCCCCCHHCCHHHHHHEEEEEEEECCC
AHYGVAMLSKVAPLKVQKGFATDEEDAQRRMIIGTFAQANGHPLTVLNGYFPQGESIDHP
HHHHHHHHHHCCCEEEECCCCCCHHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKYPAKRKFYQDLMAHLHANHSNDEDIAIIGDINISPIDLDIGIGEVNRKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHCCCCCCC
QPEEREWLKTLQGWGLVDTFRQLHPDRCERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
CCHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCCHHCCCCCCCCCCEEEEEEECHHHHH
RLVESDVDYELRGIDKPSDHAPIWSTFKEA
HHHHCCCCEEEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]