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The map label for this gene is hyfR [H]

Identifier: 117919936

GI number: 117919936

Start: 1733503

End: 1735029

Strand: Reverse

Name: hyfR [H]

Synonym: Shewana3_1488

Alternate gene names: 117919936

Gene position: 1735029-1733503 (Counterclockwise)

Preceding gene: 117919940

Following gene: 117919933

Centisome position: 34.89

GC content: 48.26

Gene sequence:

>1527_bases
ATGGAAAAACACTTCTATTACGAATCAACCCTAAGACTCTGTAGCAGTCTGCAGCTCGAAAAATCGTTGCACAGTTACTT
CGAGTTTATTCGGAATGAACTGCCTATTGACGGGCTATTTGTGAATATTTTTCGCAAACAATTTAATGATATACAGTTTA
TCGCCCATGTTGATGATAGCCATTGCTGCACGTTAGATAAGCGCATCCAAATTCCTGAGAAGATGGCGCAATACTTAGAA
GATCCCGAGCGACCAAGGGTTAGAATTGTTAACGACATAGAGCATGATCCTGTCACCTCTTTAGTCGCACCACAGGTGAT
TAAAGACATTCGCTCGGTGATTATTCTGCGAATGTCGATAGAAAATACCCACCTTGGGATAGTTGGCTTTTATGCCAAAA
GCACTGGAGTGTTTAGGCCCCGCCATGCCGACTTAATCGCGCCGCATATGCAAAACCTAGCACTGATCACGGCGCTGAAT
TTAAGGGATCGCAACTTACTCAAAGAGAACGAAGCGCTCTCGAAGCAAAACGTGAGTCTGCGTCGTACCTTAGATATTCA
AAATGGCGTGGTTGGCGCCAATTCTGGCCTGAAACAGGTCATGCAACAGGTGCAATCTATCGCCAAAATCAACACCACAG
TGTTAATGCTTGGGGAAACGGGCTGCGGCAAGGAAGTCATCGCCAACGCCATTCACGAATTGTCGGAGCGCTCGGGTAAG
CCTTTTATCAAGGTAAACTGCGGCGCCATCCCAGATACCTTGATTGACTCCGAGCTTTTTGGTTATGAAAAAGGCGCATT
TACCGGCGCCGATTCGCGCAAAGTTGGCTACTTCGAGCAGGCCAACGGCGGCACCATTTTCTTAGATGAGATAGGGGAAT
TGCCGCTTTCGGCTCAAGTTCGACTACTTAGGGTACTGCAAAATAGTTGTATCACCCGCGTTGGCGGTCACGATGCGGTT
CAGCTCGATATTCGTATTGTGGCCGCAACCCACAGAAACCTTCAGGCCATGGTGCACAGAGAAGTGTTTCGCGAGGATCT
CTGGTATCGGCTGGCGATTTTCCCCATCGAGATCCCATCACTCAGACAAAGACGCACAGATATACCTCTGCTGGTACAAC
ATTTTATCGAGCAGCTCAGTGCCAAATTCAATTTACCGCAGCTGCCAAGAATCGCCCCAGAACAACTGAATATTCTCAAC
CAATATGGTTGGCCAGGGAATGTGCGCGAACTCATTAATGTGCTCGAGCGCGCCATCATTCAGCACCCTGCAGGGCCGCT
GGATTTTAGTTTTCTAGCACCAAAGAGCCTCGAAGCCGTTAAAACGCCATCCAATCAAACCGTGATTATCGATCCAAGTC
ATGCCAGCGATAAGCTGGTGCCGCTGGAAGTGATGACCAAAAAATACATCAGCCATGCCTTAAGGATCACGGGGGGTAAA
CTCTATGGCCCAGGCGGCGCCGCCGAGTTGCTGGATATCAATGCCAATACCCTGCGCAGTAAAATGAAGAAGTTAGGGAT
TGTGTAA

Upstream 100 bases:

>100_bases
TAAAAAGAGGTATTTATGCGGTGAACTTTGATTGCAGGCACAAAATCACGAAATATAAAGATCTACAATCACGATACTTA
GTGATTAACTTGAAATATCA

Downstream 100 bases:

>100_bases
TGCATTGATAAGAAATGGATAGTTATGGAAAAGGCCACAATATGTGGCCTTTGCTTTATGTGCTTTTTTAGCGCAATCAG
TCGTCGAACTTAGAAGAAAT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MEKHFYYESTLRLCSSLQLEKSLHSYFEFIRNELPIDGLFVNIFRKQFNDIQFIAHVDDSHCCTLDKRIQIPEKMAQYLE
DPERPRVRIVNDIEHDPVTSLVAPQVIKDIRSVIILRMSIENTHLGIVGFYAKSTGVFRPRHADLIAPHMQNLALITALN
LRDRNLLKENEALSKQNVSLRRTLDIQNGVVGANSGLKQVMQQVQSIAKINTTVLMLGETGCGKEVIANAIHELSERSGK
PFIKVNCGAIPDTLIDSELFGYEKGAFTGADSRKVGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSCITRVGGHDAV
QLDIRIVAATHRNLQAMVHREVFREDLWYRLAIFPIEIPSLRQRRTDIPLLVQHFIEQLSAKFNLPQLPRIAPEQLNILN
QYGWPGNVRELINVLERAIIQHPAGPLDFSFLAPKSLEAVKTPSNQTVIIDPSHASDKLVPLEVMTKKYISHALRITGGK
LYGPGGAAELLDINANTLRSKMKKLGIV

Sequences:

>Translated_508_residues
MEKHFYYESTLRLCSSLQLEKSLHSYFEFIRNELPIDGLFVNIFRKQFNDIQFIAHVDDSHCCTLDKRIQIPEKMAQYLE
DPERPRVRIVNDIEHDPVTSLVAPQVIKDIRSVIILRMSIENTHLGIVGFYAKSTGVFRPRHADLIAPHMQNLALITALN
LRDRNLLKENEALSKQNVSLRRTLDIQNGVVGANSGLKQVMQQVQSIAKINTTVLMLGETGCGKEVIANAIHELSERSGK
PFIKVNCGAIPDTLIDSELFGYEKGAFTGADSRKVGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSCITRVGGHDAV
QLDIRIVAATHRNLQAMVHREVFREDLWYRLAIFPIEIPSLRQRRTDIPLLVQHFIEQLSAKFNLPQLPRIAPEQLNILN
QYGWPGNVRELINVLERAIIQHPAGPLDFSFLAPKSLEAVKTPSNQTVIIDPSHASDKLVPLEVMTKKYISHALRITGGK
LYGPGGAAELLDINANTLRSKMKKLGIV
>Mature_508_residues
MEKHFYYESTLRLCSSLQLEKSLHSYFEFIRNELPIDGLFVNIFRKQFNDIQFIAHVDDSHCCTLDKRIQIPEKMAQYLE
DPERPRVRIVNDIEHDPVTSLVAPQVIKDIRSVIILRMSIENTHLGIVGFYAKSTGVFRPRHADLIAPHMQNLALITALN
LRDRNLLKENEALSKQNVSLRRTLDIQNGVVGANSGLKQVMQQVQSIAKINTTVLMLGETGCGKEVIANAIHELSERSGK
PFIKVNCGAIPDTLIDSELFGYEKGAFTGADSRKVGYFEQANGGTIFLDEIGELPLSAQVRLLRVLQNSCITRVGGHDAV
QLDIRIVAATHRNLQAMVHREVFREDLWYRLAIFPIEIPSLRQRRTDIPLLVQHFIEQLSAKFNLPQLPRIAPEQLNILN
QYGWPGNVRELINVLERAIIQHPAGPLDFSFLAPKSLEAVKTPSNQTVIIDPSHASDKLVPLEVMTKKYISHALRITGGK
LYGPGGAAELLDINANTLRSKMKKLGIV

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=474, Percent_Identity=36.9198312236287, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1789087, Length=513, Percent_Identity=34.1130604288499, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=42.4528301886792, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1788550, Length=245, Percent_Identity=49.7959183673469, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=41.25, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=38.4196185286104, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=38.0368098159509, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1788905, Length=280, Percent_Identity=40.3571428571429, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87082152, Length=234, Percent_Identity=44.8717948717949, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786524, Length=390, Percent_Identity=35.3846153846154, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1787583, Length=295, Percent_Identity=36.271186440678, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=32.6666666666667, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789828, Length=266, Percent_Identity=33.0827067669173, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57130; Mature: 57130

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHFYYESTLRLCSSLQLEKSLHSYFEFIRNELPIDGLFVNIFRKQFNDIQFIAHVDDS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCC
HCCTLDKRIQIPEKMAQYLEDPERPRVRIVNDIEHDPVTSLVAPQVIKDIRSVIILRMSI
CCCCCHHHCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
ENTHLGIVGFYAKSTGVFRPRHADLIAPHMQNLALITALNLRDRNLLKENEALSKQNVSL
CCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHCCCCE
RRTLDIQNGVVGANSGLKQVMQQVQSIAKINTTVLMLGETGCGKEVIANAIHELSERSGK
EEEEECCCCEECCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCC
PFIKVNCGAIPDTLIDSELFGYEKGAFTGADSRKVGYFEQANGGTIFLDEIGELPLSAQV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHH
RLLRVLQNSCITRVGGHDAVQLDIRIVAATHRNLQAMVHREVFREDLWYRLAIFPIEIPS
HHHHHHHHHHHHHCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHEEEEEEEEECCCH
LRQRRTDIPLLVQHFIEQLSAKFNLPQLPRIAPEQLNILNQYGWPGNVRELINVLERAII
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QHPAGPLDFSFLAPKSLEAVKTPSNQTVIIDPSHASDKLVPLEVMTKKYISHALRITGGK
HCCCCCCCEEEECCCCHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCE
LYGPGGAAELLDINANTLRSKMKKLGIV
EECCCCCCEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKHFYYESTLRLCSSLQLEKSLHSYFEFIRNELPIDGLFVNIFRKQFNDIQFIAHVDDS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCC
HCCTLDKRIQIPEKMAQYLEDPERPRVRIVNDIEHDPVTSLVAPQVIKDIRSVIILRMSI
CCCCCHHHCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
ENTHLGIVGFYAKSTGVFRPRHADLIAPHMQNLALITALNLRDRNLLKENEALSKQNVSL
CCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHCCCCE
RRTLDIQNGVVGANSGLKQVMQQVQSIAKINTTVLMLGETGCGKEVIANAIHELSERSGK
EEEEECCCCEECCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCC
PFIKVNCGAIPDTLIDSELFGYEKGAFTGADSRKVGYFEQANGGTIFLDEIGELPLSAQV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHH
RLLRVLQNSCITRVGGHDAVQLDIRIVAATHRNLQAMVHREVFREDLWYRLAIFPIEIPS
HHHHHHHHHHHHHCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHEEEEEEEEECCCH
LRQRRTDIPLLVQHFIEQLSAKFNLPQLPRIAPEQLNILNQYGWPGNVRELINVLERAII
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QHPAGPLDFSFLAPKSLEAVKTPSNQTVIIDPSHASDKLVPLEVMTKKYISHALRITGGK
HCCCCCCCEEEECCCCHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCE
LYGPGGAAELLDINANTLRSKMKKLGIV
EECCCCCCEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]