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The map label for this gene is mltF [H]

Identifier: 117919688

GI number: 117919688

Start: 1448713

End: 1450149

Strand: Reverse

Name: mltF [H]

Synonym: Shewana3_1239

Alternate gene names: 117919688

Gene position: 1450149-1448713 (Counterclockwise)

Preceding gene: 117919698

Following gene: 117919683

Centisome position: 29.17

GC content: 50.59

Gene sequence:

>1437_bases
ATGACTCGATTTCTGTTCGCGATTATCTTAGGTTTCTTACTGACGGCTTGTCAGCAAGTGACCGTGGAAGAAACAGAATA
TGTCCCCCATAAACTCACCGAATTAAGGGTCGGCACCCTTTATGGCCCGCAAATTTATATGACCTCGGGCCAAGGCAACA
GCGGCTTCGATTACGATATGGCAGTGCTCTTTGCCGAGTATCTCGATGTGCCCTTAAAAATGGTGCCCTACACCAACCGT
GCCGAACTCTATGATGCACTTAAAAAAAACGAGATTGATATTATTGCCGCGGGGATGACTGAAACCCCAGCGCGGCGCGA
ACAATTCCGTTTAGGCCCGCCGCTGTATCGCGTCAATCAAGTACTGGTTTACCGTGAAGGCATGCCAACACCCAAAGATA
TCACTGACTTAAAAGGCAAAATTACTGTAATTGCCGACTCTTCTTTTGTGGAAACCTTGACGCAATTACAGAAACGTCAT
CCGACGCTGGTGTGGGATCAAGTCACAGATAAAGACAGCGAAGAGCTGCTGGCGATGATCGCCAACAAGGAAATCGACTA
CACCATCGCCGACTCGAGCAGCGTGCAGATTAACCGCCGCTATTTACCGGATCTACGTTCGGGGCTCGTGCTAGAAGAAA
AGCTCGATGTGGTTTGGTTGCTCCCCCCCACACACAGTGACGGCTTGATGAGTCAGTTATTGGCGTTTTGGCACCAAGAA
AAACTCGCCGGTACCCTCGATCATCTTAATGAAAAGTACTTTGGCCACGTTAAGCGTTTCGACTATATCGATACCCGCGC
CTTCCTGCGCGCCATCGAAACCGTATTGCCTCGTTATCGTCAGCTTTTCGAAACCCACGCTGGTGACTTAGATTGGCGTA
AATTGGCGGCAACCAGTTATCAAGAGTCACATTGGAATCCTAATGCCCGCTCACCCACTGGGGTGCGCGGCATGATGATG
TTAACGCAGCCGACCGCCAAAGAAATTGGCATTACAAATCGACTCGATGCGGAAGAAAGTATTCGCGGCGGCGCCGCTTA
TTTACGGGATATGATCAACCGTCTGCCCGAGTCTATCCCCGAGAGTCAACGCATGTGGTTTGCGCTGGCCTCTTACAATA
TCGGTTATGCCCATGTGGAAGACGCCCGTAAACTCGCTGAGTCCATGGAGCTGAATCCTAACGCCTGGCGGGATCTTAAA
AAAGTATTGCCACTACTGCAAAAGCGTAAATATTACCAAAAGACTCGCTACGGCTATGCCCGTGGTAGCGAAGCCGTGCA
TTACGTCGACAGCATCCGCCGTTATTACGACACCTTAGTCTGGGTCGATAATCAGTCTAAACAGCAAAATAGCGATGAGG
AAGAACCAAGCGACCTCGCCTCGGAAGATGGCCCAGCGCCAGTGCCCGGCACCCTCAGCCCTGATAAGCCGAAATAG

Upstream 100 bases:

>100_bases
CAAACGGATATAATCACAGCAATGTCAGCCAGTTTATCCTTGTTACCTCACTGACCTTCTACTGTTCATCATTTTGATTT
TATTTAATAAAGTGTGTTTA

Downstream 100 bases:

>100_bases
CCCTAACCGCTTCCCCCTCAATAACTTGACTTGCGCCGCAGCCTTGCTTTTTTACGGCTTTCAAACCACGATTTTAATCT
TAGATTAAGCCAATACAGGC

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTRFLFAIILGFLLTACQQVTVEETEYVPHKLTELRVGTLYGPQIYMTSGQGNSGFDYDMAVLFAEYLDVPLKMVPYTNR
AELYDALKKNEIDIIAAGMTETPARREQFRLGPPLYRVNQVLVYREGMPTPKDITDLKGKITVIADSSFVETLTQLQKRH
PTLVWDQVTDKDSEELLAMIANKEIDYTIADSSSVQINRRYLPDLRSGLVLEEKLDVVWLLPPTHSDGLMSQLLAFWHQE
KLAGTLDHLNEKYFGHVKRFDYIDTRAFLRAIETVLPRYRQLFETHAGDLDWRKLAATSYQESHWNPNARSPTGVRGMMM
LTQPTAKEIGITNRLDAEESIRGGAAYLRDMINRLPESIPESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLK
KVLPLLQKRKYYQKTRYGYARGSEAVHYVDSIRRYYDTLVWVDNQSKQQNSDEEEPSDLASEDGPAPVPGTLSPDKPK

Sequences:

>Translated_478_residues
MTRFLFAIILGFLLTACQQVTVEETEYVPHKLTELRVGTLYGPQIYMTSGQGNSGFDYDMAVLFAEYLDVPLKMVPYTNR
AELYDALKKNEIDIIAAGMTETPARREQFRLGPPLYRVNQVLVYREGMPTPKDITDLKGKITVIADSSFVETLTQLQKRH
PTLVWDQVTDKDSEELLAMIANKEIDYTIADSSSVQINRRYLPDLRSGLVLEEKLDVVWLLPPTHSDGLMSQLLAFWHQE
KLAGTLDHLNEKYFGHVKRFDYIDTRAFLRAIETVLPRYRQLFETHAGDLDWRKLAATSYQESHWNPNARSPTGVRGMMM
LTQPTAKEIGITNRLDAEESIRGGAAYLRDMINRLPESIPESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLK
KVLPLLQKRKYYQKTRYGYARGSEAVHYVDSIRRYYDTLVWVDNQSKQQNSDEEEPSDLASEDGPAPVPGTLSPDKPK
>Mature_477_residues
TRFLFAIILGFLLTACQQVTVEETEYVPHKLTELRVGTLYGPQIYMTSGQGNSGFDYDMAVLFAEYLDVPLKMVPYTNRA
ELYDALKKNEIDIIAAGMTETPARREQFRLGPPLYRVNQVLVYREGMPTPKDITDLKGKITVIADSSFVETLTQLQKRHP
TLVWDQVTDKDSEELLAMIANKEIDYTIADSSSVQINRRYLPDLRSGLVLEEKLDVVWLLPPTHSDGLMSQLLAFWHQEK
LAGTLDHLNEKYFGHVKRFDYIDTRAFLRAIETVLPRYRQLFETHAGDLDWRKLAATSYQESHWNPNARSPTGVRGMMML
TQPTAKEIGITNRLDAEESIRGGAAYLRDMINRLPESIPESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLKK
VLPLLQKRKYYQKTRYGYARGSEAVHYVDSIRRYYDTLVWVDNQSKQQNSDEEEPSDLASEDGPAPVPGTLSPDKPK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=445, Percent_Identity=42.9213483146067, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 54801; Mature: 54670

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFLFAIILGFLLTACQQVTVEETEYVPHKLTELRVGTLYGPQIYMTSGQGNSGFDYDM
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEECCEEEEECCCCCCCCCHHH
AVLFAEYLDVPLKMVPYTNRAELYDALKKNEIDIIAAGMTETPARREQFRLGPPLYRVNQ
HHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCHHHHCE
VLVYREGMPTPKDITDLKGKITVIADSSFVETLTQLQKRHPTLVWDQVTDKDSEELLAMI
EEEEECCCCCCCCHHCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
ANKEIDYTIADSSSVQINRRYLPDLRSGLVLEEKLDVVWLLPPTHSDGLMSQLLAFWHQE
HCCCCCEEEECCCCEEECHHHCCHHHHCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
KLAGTLDHLNEKYFGHVKRFDYIDTRAFLRAIETVLPRYRQLFETHAGDLDWRKLAATSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCH
QESHWNPNARSPTGVRGMMMLTQPTAKEIGITNRLDAEESIRGGAAYLRDMINRLPESIP
HHHCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCC
ESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLKKVLPLLQKRKYYQKTRYGYA
CHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGSEAVHYVDSIRRYYDTLVWVDNQSKQQNSDEEEPSDLASEDGPAPVPGTLSPDKPK
CCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRFLFAIILGFLLTACQQVTVEETEYVPHKLTELRVGTLYGPQIYMTSGQGNSGFDYDM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEECCEEEEECCCCCCCCCHHH
AVLFAEYLDVPLKMVPYTNRAELYDALKKNEIDIIAAGMTETPARREQFRLGPPLYRVNQ
HHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCHHHHCE
VLVYREGMPTPKDITDLKGKITVIADSSFVETLTQLQKRHPTLVWDQVTDKDSEELLAMI
EEEEECCCCCCCCHHCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
ANKEIDYTIADSSSVQINRRYLPDLRSGLVLEEKLDVVWLLPPTHSDGLMSQLLAFWHQE
HCCCCCEEEECCCCEEECHHHCCHHHHCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
KLAGTLDHLNEKYFGHVKRFDYIDTRAFLRAIETVLPRYRQLFETHAGDLDWRKLAATSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCH
QESHWNPNARSPTGVRGMMMLTQPTAKEIGITNRLDAEESIRGGAAYLRDMINRLPESIP
HHHCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCC
ESQRMWFALASYNIGYAHVEDARKLAESMELNPNAWRDLKKVLPLLQKRKYYQKTRYGYA
CHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGSEAVHYVDSIRRYYDTLVWVDNQSKQQNSDEEEPSDLASEDGPAPVPGTLSPDKPK
CCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]