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The map label for this gene is yafC [C]

Identifier: 117919635

GI number: 117919635

Start: 1393260

End: 1394201

Strand: Direct

Name: yafC [C]

Synonym: Shewana3_1186

Alternate gene names: 117919635

Gene position: 1393260-1394201 (Clockwise)

Preceding gene: 117919634

Following gene: 117919644

Centisome position: 28.02

GC content: 48.83

Gene sequence:

>942_bases
ATGTTAGAACTGTTAGAACCTATTGCGATTTTTACTCATGTGGCCAGAGCTGGTAGTTTTAGTGCCGCCGCAAGAAGGCT
TGGGATCTCTAAATCTAAGGTCAGTACGCAGGTCGCCGATCTCGAGCATAGACTCGGTGTTCAACTGATCCAGCGTACCA
CCCGAAGCTTAAGTCTGACTGAAGCTGGACATTTGTTATATCTGCAAGGTGAAGAGTTGCTCCGCGATGCCGAACAGGCC
ATTGCTAGCGTGCATAACTTAAACGATGCCACCCGCGGCGTGTTAAAGGTGGGGATTTCCCAGTCCTTTGGCGCTATGCA
TATCATTCCCGCACTGCCATCATTTATGGCAAAACACCCTGAGCTAGAGCTGCAAGTCAGCTTATTAGATCATAAGGTGG
ATGTGGTCAGCGAAGGTTTAGACCTATTGTTGACTATGTCTGAGCAGCTTCCTCTCGGTATGGTGGCGCGCCCTTTGATG
AAGTGTCAGTTCCTCCTCGTTGCCTCCCCCGATTACATTGCGAAACATGGCGAACCGTCGCGCCCTGAACAATTGGTCGA
CCATAACTGCTTGGTGTATCAAGGCGAGTGGCATGAGCACAGCATGTGGCAGTTTAAAAAGGGCGATGATTACTGTGAGA
TAGGTGTCTCTGGTAACTTTAGAGTGGATAACGCTCCTGCTTTAAAATCAGCCGCAATCAGTGGCTTAGGGGTTGTATAT
CTTGCCAGCTACTTGATGGAAGATGAAATCGCTAAGGGCACCTTAGTGCCGCTGCTGAGGGATTGGCAACTGACTCATCA
TTTACCGCTACAGGCCGTTTACCCACGCCGTAAACACTTAGCGCCTAAGGTGAGTGCTTTCATTGAGTTTATTAAAGACC
ACATTGGTAATCCGCCTTATTGGGACATTCCCTATGCGGAACTCTTCAGTGAACGTCAGTAA

Upstream 100 bases:

>100_bases
GATATTAAGGCATTGAGGGATGCTTTGAGGCATTTGTGCTGCATTCCTATGCTATTCCGAAAGTTAATATCACTGTCGAA
ACTGCTTAAGGCGACTTTCC

Downstream 100 bases:

>100_bases
TTTTGGAAACATTGTTCTTAAAATGCGGTTAATGCATTTCTAGAGAGTTTGATGGTTTTGAAATTCATTTAAAATCAATT
ATTTGCGTCGAGATTAGTTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQA
IASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLVASPDYIAKHGEPSRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
LASYLMEDEIAKGTLVPLLRDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIGNPPYWDIPYAELFSERQ

Sequences:

>Translated_313_residues
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQA
IASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLVASPDYIAKHGEPSRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
LASYLMEDEIAKGTLVPLLRDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIGNPPYWDIPYAELFSERQ
>Mature_313_residues
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQA
IASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLVASPDYIAKHGEPSRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
LASYLMEDEIAKGTLVPLLRDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIGNPPYWDIPYAELFSERQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=30.976430976431, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=30.1369863013699, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789440, Length=299, Percent_Identity=26.7558528428094, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=30.5084745762712, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=27.4914089347079, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=31.1188811188811, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789639, Length=301, Percent_Identity=27.2425249169435, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=26.2975778546713, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788706, Length=304, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788297, Length=303, Percent_Identity=26.4026402640264, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790011, Length=291, Percent_Identity=25.4295532646048, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=305, Percent_Identity=26.5573770491803, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=33.6134453781513, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790783, Length=293, Percent_Identity=24.9146757679181, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788748, Length=121, Percent_Identity=34.7107438016529, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=26.6272189349112, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34910; Mature: 34910

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
EAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHP
CCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCC
ELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPDYIAKHGEPS
CCEEEEHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHEEEEEEECCHHHHCCCCCC
RPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
CCHHHHCCCEEEEECCCCCCCCEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LASYLMEDEIAKGTLVPLLRDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIGNPPY
HHHHHHHHHHCCCHHHHHHHHCHHHHCCCCHHCCCHHHHCCHHHHHHHHHHHHHCCCCCC
WDIPYAELFSERQ
CCCCHHHHHCCCC
>Mature Secondary Structure
MLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRSLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
EAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHP
CCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCC
ELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPDYIAKHGEPS
CCEEEEHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHEEEEEEECCHHHHCCCCCC
RPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGVSGNFRVDNAPALKSAAISGLGVVY
CCHHHHCCCEEEEECCCCCCCCEEECCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LASYLMEDEIAKGTLVPLLRDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIGNPPY
HHHHHHHHHHCCCHHHHHHHHCHHHHCCCCHHCCCHHHHCCHHHHHHHHHHHHHCCCCCC
WDIPYAELFSERQ
CCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]