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The map label for this gene is nemA [H]

Identifier: 117919608

GI number: 117919608

Start: 1365887

End: 1366954

Strand: Direct

Name: nemA [H]

Synonym: Shewana3_1159

Alternate gene names: 117919608

Gene position: 1365887-1366954 (Clockwise)

Preceding gene: 117919606

Following gene: 117919610

Centisome position: 27.47

GC content: 49.63

Gene sequence:

>1068_bases
ATGACGATTGAACATGCAGCGAATTCCGTGGGTAATTTATTCGATACCTACAAACTTAATGACACTATTACCTTAAAAAA
CCGTATCTTAATGGCGCCATTAACGCGCTGTATGGCCGATGCGGATTTAGTGCCGACCGATGACATGGTGGCTTACTATG
CTCGCCGCGCCGAAGCGGGATTGATTATCTCCGAAGCGACGATCATTCGCCCTGATGGTCAAGGTTACCCAAATACGCCC
GGCATTTTTAATCAAGCGCAGATCGCCGGTTGGCGTAAAGTAACGGATGGTGTGCATGCCAATGGCGGCAAGATTTTTGT
GCAGTTATGGCATACCGGCCGTGTCGCGCACCCACATTTCTTCGGTGGCGGCGATGTACTGGCGCCTTCGGCAGAAAAAG
TAGAGGGCAGTGTGCCCAGAATGCGTGAGCTGATCTATGTCACTCCAAAAGCGGCAACTCTTGAAGATATTCAGGGCTTA
GTCCGCGATTATGCGAAAGCGGCTGAAAACGCGATTGAAGCCGGATTCGATGGGGTTGAGATCCACGGTGCAAACGGATA
TCTAATCGATGCATTCTTACACCATGACAGCAACCGTCGTACCGATGAATATGGCGGCACACCTGCCAATATGTCACGCT
TTGCCTTAGAAGTGGTTGATGCGATTGCGGCGCGTATTGGTAAAGACAGAACCGGTCTTCGTATTTCTCCCGGCGCCTAT
TTCAATATGGCTGTCGATAACCGTGACCGCGCCGTGTTTGACTACCTATTACCTGAGCTTGAAAAACGTGAACTGGCCTT
TGTGCATATCGGTATCTTCGACGACAGCATGGAGTTTGATTACTTAGATGGCCGTGTTTCAAGCTATGTGCGTGCTAACT
ATGGCAAAACCTTAGTGGGTGTAGGTGGCTACAGCGCCCAATCGGCAAGTGAAGCGATTGCCGCCGATAAGTTTGATTTA
ATTGCGATTGGTCGCCCCTTTATTGCCAACCCTGATTATGTGGCAAGAGTGCGCCAAGGTCATGAATTGGTGACCTATAG
CGATGAAATGCTGGCAAGCTTGGTGTAA

Upstream 100 bases:

>100_bases
GCTGATTCTAACTAATTTTGAACAAACATTCAAAAATGTTCTTGAATGATCGTTCAAATAAGAATATCTTGAATGCACAT
TCAAGAATGGGGCAACACCA

Downstream 100 bases:

>100_bases
CTCAGAGCAGCAATTACCGATAAGAAAGGGTTAGCTTAGCTAACCCTTTTGCTTTTGGTTCAACGATCAATTGGCTTTAG
GGAGCGCCCTCAGGGCATTG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTIEHAANSVGNLFDTYKLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAGLIISEATIIRPDGQGYPNTP
GIFNQAQIAGWRKVTDGVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAEKVEGSVPRMRELIYVTPKAATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVGVGGYSAQSASEAIAADKFDL
IAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV

Sequences:

>Translated_355_residues
MTIEHAANSVGNLFDTYKLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAGLIISEATIIRPDGQGYPNTP
GIFNQAQIAGWRKVTDGVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAEKVEGSVPRMRELIYVTPKAATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVGVGGYSAQSASEAIAADKFDL
IAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV
>Mature_354_residues
TIEHAANSVGNLFDTYKLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAGLIISEATIIRPDGQGYPNTPG
IFNQAQIAGWRKVTDGVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAEKVEGSVPRMRELIYVTPKAATLEDIQGLV
RDYAKAAENAIEAGFDGVEIHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAYF
NMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVGVGGYSAQSASEAIAADKFDLI
AIGRPFIANPDYVARVRQGHELVTYSDEMLASLV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=345, Percent_Identity=43.1884057971014, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789463, Length=344, Percent_Identity=29.3604651162791, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=355, Percent_Identity=26.7605633802817, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=348, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=353, Percent_Identity=27.7620396600567, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=363, Percent_Identity=26.1707988980716, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=370, Percent_Identity=25.9459459459459, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=359, Percent_Identity=25.6267409470752, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=363, Percent_Identity=37.1900826446281, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321973, Length=363, Percent_Identity=35.5371900826446, Blast_Score=183, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38880; Mature: 38749

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEHAANSVGNLFDTYKLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAG
CCCHHHHHHHHHHHHEEECCCEEEECCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LIISEATIIRPDGQGYPNTPGIFNQAQIAGWRKVTDGVHANGGKIFVQLWHTGRVAHPHF
EEEECEEEECCCCCCCCCCCCCCCCHHHCCHHHHCCCEECCCCEEEEEEECCCCCCCCEE
FGGGDVLAPSAEKVEGSVPRMRELIYVTPKAATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEECCCHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVG
EEEEECCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEE
VGGYSAQSASEAIAADKFDLIAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV
ECCCCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEEEEECHHHHHHCC
>Mature Secondary Structure 
TIEHAANSVGNLFDTYKLNDTITLKNRILMAPLTRCMADADLVPTDDMVAYYARRAEAG
CCHHHHHHHHHHHHEEECCCEEEECCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LIISEATIIRPDGQGYPNTPGIFNQAQIAGWRKVTDGVHANGGKIFVQLWHTGRVAHPHF
EEEECEEEECCCCCCCCCCCCCCCCHHHCCHHHHCCCEECCCCEEEEEEECCCCCCCCEE
FGGGDVLAPSAEKVEGSVPRMRELIYVTPKAATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEECCCHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDAFLHHDSNRRTDEYGGTPANMSRFALEVVDAIAARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAVDNRDRAVFDYLLPELEKRELAFVHIGIFDDSMEFDYLDGRVSSYVRANYGKTLVG
EEEEECCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEE
VGGYSAQSASEAIAADKFDLIAIGRPFIANPDYVARVRQGHELVTYSDEMLASLV
ECCCCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCEEEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]