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The map label for this gene is amtB [H]

Identifier: 117919230

GI number: 117919230

Start: 900554

End: 901954

Strand: Reverse

Name: amtB [H]

Synonym: Shewana3_0778

Alternate gene names: 117919230

Gene position: 901954-900554 (Counterclockwise)

Preceding gene: 117919231

Following gene: 117919223

Centisome position: 18.14

GC content: 53.32

Gene sequence:

>1401_bases
ATGAGCCAACAGACAGCCGCACTTAACGCTAAGCTAGAGCGCAGCACGAATATTCCAAACGGCCAGCACGCTAAGTTCGC
TCAGCCAAACGCCCGTTATTCAAAATTGGGTTATCAGGCATTATTGGCTCTGCTCACCAGCGGCTTTGCGGGCTCGGTTT
TGGCCGATGAGGCGCAGCTCAGCGGCGCCAACACCGCATGGATTTTAAGCTCATCCGCCCTCGTCTTATTGATGACACTC
CCTGGACTGGCGCTGTTTTACGGCGGTCTGGTGCGCAGTAAAAACGTACTCTCGATTTTAATGCAGTGTTTTTCTATCGC
TGCTATCGCCTCTGTACTGTGGTTTGTGGTGGGTTACTCCATCGCCTTCGATACCGGCAACGGTTTTATCGGCGGGCTTG
GCAAAGCTTTTCTAGCCAGCATTGGTCGCGATAGTGTCAGTGGGGATATTCCTGAACCTCTGTTTATGCTTTTCCAGATG
ACCTTCGCGATTATCACGCCAGCGCTGATCATCGGTGGCTTTGCCGAGCGGATGAGGTTCTCCGCCGTACTGATGTTTTC
AAGCGCTTGGTTGCTGTTAGTCTATGCACCTATCACGCACTGGGTGTGGGGCGGTGGCTGGTTGGCCGAGCTTGGATTGT
ATGACTTTGCTGGCGGTACAGTCGTACACATTACCGCTGGGGTGGCGGCACTGGTGGCGGCAAAAGTATTAGGGCCGCGT
AAGGGATTTTTAAACTCGGCCATTATGCCCCATAACTTGACCATGACAGTGACAGGCGCGGGCATGCTGTGGGTAGGCTG
GTTTGGCTTTAATGGCGGCAGCGCTTTAGGCGCCAATGGCACTGCGGCCATGGCGATTCTCGCGACCCATTTAGCCGCGT
CGATGGGCGCCATTACTTGGGCGGGTATCGAGTGGTATAAATTCGGTAAGGCCAGTGCACTCGGTATCGTCACTGGCATG
GTGGCAGGCCTTGGCACTATCACGCCCGCATCGGGTTATGTCGGCCCAGCTGGCGCATTAGTGATAGGTTTTCTTGGCGG
CGTGGTTTGCTTTTTTTCAACTGTTTATATTAAACAAAAGCTTAAGATTGACGACTCACTCGATGTGTTCCCAGTGCATG
GTGTCGGCGGTATTCTCGGCACTTTACTGGCGGGGGTATTTAGTTCGACTCAGCTTGGGATCTTCAGCGGTTATGGCTTT
GCCAGTGTTAACCCCACTATGCTCGACCAACTCGGAGCGCAGGCCATTGGGGTTATTGCCACCTTGAGTTACACCGCCCT
GGTGTCTTGGTTGTTGTTTGTGGTCATTGGCAAAATCCTCGGCGGACTTCGTGTCAGTACCGAACAGGAAGTGAATGGCC
TCGATCTTTCCGAACATGAAGAAACAGGCTACAGCCTCTAA

Upstream 100 bases:

>100_bases
CCGCCGCTCACACGGGTAAAGTCGGTGACGGAAAGATATTCGTCACCCCACTCGAACAAGTGATCCGCGTGCGCACCTCG
GAAGAAGGGAGTGATGCGAT

Downstream 100 bases:

>100_bases
TACTCGCCTCGCAAAACTGACCGTCAGGGGCATATCTGCCCTGACGGTTATTAACTAGTTATGCTCCTTAAGCCACAGTT
CCAGTTCATTTAATTTCATC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSQQTAALNAKLERSTNIPNGQHAKFAQPNARYSKLGYQALLALLTSGFAGSVLADEAQLSGANTAWILSSSALVLLMTL
PGLALFYGGLVRSKNVLSILMQCFSIAAIASVLWFVVGYSIAFDTGNGFIGGLGKAFLASIGRDSVSGDIPEPLFMLFQM
TFAIITPALIIGGFAERMRFSAVLMFSSAWLLLVYAPITHWVWGGGWLAELGLYDFAGGTVVHITAGVAALVAAKVLGPR
KGFLNSAIMPHNLTMTVTGAGMLWVGWFGFNGGSALGANGTAAMAILATHLAASMGAITWAGIEWYKFGKASALGIVTGM
VAGLGTITPASGYVGPAGALVIGFLGGVVCFFSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGIFSGYGF
ASVNPTMLDQLGAQAIGVIATLSYTALVSWLLFVVIGKILGGLRVSTEQEVNGLDLSEHEETGYSL

Sequences:

>Translated_466_residues
MSQQTAALNAKLERSTNIPNGQHAKFAQPNARYSKLGYQALLALLTSGFAGSVLADEAQLSGANTAWILSSSALVLLMTL
PGLALFYGGLVRSKNVLSILMQCFSIAAIASVLWFVVGYSIAFDTGNGFIGGLGKAFLASIGRDSVSGDIPEPLFMLFQM
TFAIITPALIIGGFAERMRFSAVLMFSSAWLLLVYAPITHWVWGGGWLAELGLYDFAGGTVVHITAGVAALVAAKVLGPR
KGFLNSAIMPHNLTMTVTGAGMLWVGWFGFNGGSALGANGTAAMAILATHLAASMGAITWAGIEWYKFGKASALGIVTGM
VAGLGTITPASGYVGPAGALVIGFLGGVVCFFSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGIFSGYGF
ASVNPTMLDQLGAQAIGVIATLSYTALVSWLLFVVIGKILGGLRVSTEQEVNGLDLSEHEETGYSL
>Mature_465_residues
SQQTAALNAKLERSTNIPNGQHAKFAQPNARYSKLGYQALLALLTSGFAGSVLADEAQLSGANTAWILSSSALVLLMTLP
GLALFYGGLVRSKNVLSILMQCFSIAAIASVLWFVVGYSIAFDTGNGFIGGLGKAFLASIGRDSVSGDIPEPLFMLFQMT
FAIITPALIIGGFAERMRFSAVLMFSSAWLLLVYAPITHWVWGGGWLAELGLYDFAGGTVVHITAGVAALVAAKVLGPRK
GFLNSAIMPHNLTMTVTGAGMLWVGWFGFNGGSALGANGTAAMAILATHLAASMGAITWAGIEWYKFGKASALGIVTGMV
AGLGTITPASGYVGPAGALVIGFLGGVVCFFSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGIFSGYGFA
SVNPTMLDQLGAQAIGVIATLSYTALVSWLLFVVIGKILGGLRVSTEQEVNGLDLSEHEETGYSL

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=415, Percent_Identity=46.0240963855422, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=30.7692307692308, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=29.0322580645161, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32564042, Length=431, Percent_Identity=27.3781902552204, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=26.8348623853211, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=424, Percent_Identity=37.9716981132075, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=34.6153846153846, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=32.2115384615385, Blast_Score=202, Evalue=7e-53,
Organism=Drosophila melanogaster, GI161078314, Length=423, Percent_Identity=29.7872340425532, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 48449; Mature: 48318

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQTAALNAKLERSTNIPNGQHAKFAQPNARYSKLGYQALLALLTSGFAGSVLADEAQL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCHHHC
SGANTAWILSSSALVLLMTLPGLALFYGGLVRSKNVLSILMQCFSIAAIASVLWFVVGYS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFDTGNGFIGGLGKAFLASIGRDSVSGDIPEPLFMLFQMTFAIITPALIIGGFAERMRF
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVLMFSSAWLLLVYAPITHWVWGGGWLAELGLYDFAGGTVVHITAGVAALVAAKVLGPR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHCCCCCEEEEEHHHHHHHHHHHHHCCC
KGFLNSAIMPHNLTMTVTGAGMLWVGWFGFNGGSALGANGTAAMAILATHLAASMGAITW
HHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
AGIEWYKFGKASALGIVTGMVAGLGTITPASGYVGPAGALVIGFLGGVVCFFSTVYIKQK
CCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGIFSGYGFASVNPTMLDQLGAQAIGVIA
HCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCHHHHHHHH
TLSYTALVSWLLFVVIGKILGGLRVSTEQEVNGLDLSEHEETGYSL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQQTAALNAKLERSTNIPNGQHAKFAQPNARYSKLGYQALLALLTSGFAGSVLADEAQL
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCHHHC
SGANTAWILSSSALVLLMTLPGLALFYGGLVRSKNVLSILMQCFSIAAIASVLWFVVGYS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFDTGNGFIGGLGKAFLASIGRDSVSGDIPEPLFMLFQMTFAIITPALIIGGFAERMRF
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVLMFSSAWLLLVYAPITHWVWGGGWLAELGLYDFAGGTVVHITAGVAALVAAKVLGPR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHCCCCCEEEEEHHHHHHHHHHHHHCCC
KGFLNSAIMPHNLTMTVTGAGMLWVGWFGFNGGSALGANGTAAMAILATHLAASMGAITW
HHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
AGIEWYKFGKASALGIVTGMVAGLGTITPASGYVGPAGALVIGFLGGVVCFFSTVYIKQK
CCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGIFSGYGFASVNPTMLDQLGAQAIGVIA
HCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCHHHHHHHH
TLSYTALVSWLLFVVIGKILGGLRVSTEQEVNGLDLSEHEETGYSL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]