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The map label for this gene is lolB [H]

Identifier: 117919219

GI number: 117919219

Start: 889452

End: 890096

Strand: Reverse

Name: lolB [H]

Synonym: Shewana3_0767

Alternate gene names: 117919219

Gene position: 890096-889452 (Counterclockwise)

Preceding gene: 117919223

Following gene: 117919218

Centisome position: 17.9

GC content: 51.47

Gene sequence:

>645_bases
ATGAATAATTTGAAGCGCTTCACAAAATCCATTTTCTCTTGTATCGCCTTGAGCGGCCTATTGTTTTTAGGCGGCTGTGA
AACCCTGCCGCCAACGACAGATCTCAGCCCTATGACGGTCGACAATGCCGCCCAAGCCAAGGCATGGGAATTACAGGGGA
AACTCGCTATCCGCACGCCACAGGATAAGCTCAGCGCCAATCTCTATTGGCGCCACAGTGAGGAGCGAGACGAACTCACC
CTCACCACTATGCTCGGCACCACAGTGTTGACCTTGGATGCTACGCCTAACTCGGCTCACCTGCATGTCGATGGCAAAGA
TTTTCGTGATAGCAACGCCCAAGCATTACTCGAGCGAGTCAGCGGCTGGTCCATCCCGATTAACGACTTACCCCTTTGGA
TAACAGGCCAAGTCGGCGCTTTAGACCGTGTGCTTGCCGTCGATAGCAATGGCAAAACCAAACAAATTCAAAACTCGCAA
ACCTTGCCGCCTTGGGTCGTCACATTTTTGAGTTGGCAGCCCCAAAGCGGTGCCGAGGTGCCCTATCAGTTAAAGCTTGA
ACGTGGTGATTTACAGCTCAAGCTGCAACTCAATCAATGGCAAGCCTTAGGTAAACCCTCAATACTGCTCGGAGAAAAAC
CTTAG

Upstream 100 bases:

>100_bases
CCGAAGTTATCCCAAGTGACCGCCAATGGAAATTTTACGATTGGCATTGGTATTCTTTACAAGCCCTCGTATCATAAGGA
CTTTGGTATATCAGTTATAG

Downstream 100 bases:

>100_bases
CATGTCGAATGAGATTTCACGCAACTGGCCCGCGCCCGCCAAATTAAATCTGTTTTTGCATATCAACGGGCGCCGCGCCG
ACGGTTACCATGAGTTGCAA

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNNLKRFTKSIFSCIALSGLLFLGGCETLPPTTDLSPMTVDNAAQAKAWELQGKLAIRTPQDKLSANLYWRHSEERDELT
LTTMLGTTVLTLDATPNSAHLHVDGKDFRDSNAQALLERVSGWSIPINDLPLWITGQVGALDRVLAVDSNGKTKQIQNSQ
TLPPWVVTFLSWQPQSGAEVPYQLKLERGDLQLKLQLNQWQALGKPSILLGEKP

Sequences:

>Translated_214_residues
MNNLKRFTKSIFSCIALSGLLFLGGCETLPPTTDLSPMTVDNAAQAKAWELQGKLAIRTPQDKLSANLYWRHSEERDELT
LTTMLGTTVLTLDATPNSAHLHVDGKDFRDSNAQALLERVSGWSIPINDLPLWITGQVGALDRVLAVDSNGKTKQIQNSQ
TLPPWVVTFLSWQPQSGAEVPYQLKLERGDLQLKLQLNQWQALGKPSILLGEKP
>Mature_214_residues
MNNLKRFTKSIFSCIALSGLLFLGGCETLPPTTDLSPMTVDNAAQAKAWELQGKLAIRTPQDKLSANLYWRHSEERDELT
LTTMLGTTVLTLDATPNSAHLHVDGKDFRDSNAQALLERVSGWSIPINDLPLWITGQVGALDRVLAVDSNGKTKQIQNSQ
TLPPWVVTFLSWQPQSGAEVPYQLKLERGDLQLKLQLNQWQALGKPSILLGEKP

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=156, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 23685; Mature: 23685

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKRFTKSIFSCIALSGLLFLGGCETLPPTTDLSPMTVDNAAQAKAWELQGKLAIRTP
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEECCCCCCEEEEECCEEEEECC
QDKLSANLYWRHSEERDELTLTTMLGTTVLTLDATPNSAHLHVDGKDFRDSNAQALLERV
HHHHCEEEEEECCCCCCCEEEEEEECEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHH
SGWSIPINDLPLWITGQVGALDRVLAVDSNGKTKQIQNSQTLPPWVVTFLSWQPQSGAEV
CCCCCCCCCCCEEEECCCCCCEEEEEECCCCCEEECCCCCCCCHHHEEEEECCCCCCCCC
PYQLKLERGDLQLKLQLNQWQALGKPSILLGEKP
CEEEEEECCCEEEEEEECCHHCCCCCCEEECCCC
>Mature Secondary Structure
MNNLKRFTKSIFSCIALSGLLFLGGCETLPPTTDLSPMTVDNAAQAKAWELQGKLAIRTP
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEECCCCCCEEEEECCEEEEECC
QDKLSANLYWRHSEERDELTLTTMLGTTVLTLDATPNSAHLHVDGKDFRDSNAQALLERV
HHHHCEEEEEECCCCCCCEEEEEEECEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHH
SGWSIPINDLPLWITGQVGALDRVLAVDSNGKTKQIQNSQTLPPWVVTFLSWQPQSGAEV
CCCCCCCCCCCEEEECCCCCCEEEEEECCCCCEEECCCCCCCCHHHEEEEECCCCCCCCC
PYQLKLERGDLQLKLQLNQWQALGKPSILLGEKP
CEEEEEECCCEEEEEEECCHHCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]