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The map label for this gene is rpoN [H]

Identifier: 117919124

GI number: 117919124

Start: 791964

End: 793439

Strand: Reverse

Name: rpoN [H]

Synonym: Shewana3_0672

Alternate gene names: 117919124

Gene position: 793439-791964 (Counterclockwise)

Preceding gene: 117919125

Following gene: 117919123

Centisome position: 15.96

GC content: 48.78

Gene sequence:

>1476_bases
ATGAAAGCGTCACTCCAGCTCAAACTGGGTCAACAGTTAACCATGACGCCACAACTTCAACAGGCCATTCGTCTGTTGCA
ACTGTCGTCATTGGAACTGCAGCAAGAGATCCAGCAAGCCTTAGACTCTAATCCTCTTCTTGAGCTGGAAGAGGAGCAAT
TCGACGCGCCTACCGAACGTAAGCAAGATATCGCCGATACCGACTTTAGCGAAACGACAGCTCAGCCACAGGAACAAGAT
AATTCCACCGTGGACATTGCCGAGTCGTTGACCAAAGAATCCCTGCCCGAAGAACTACCCATGGACACCACTTGGGATGA
AGTCTTTACCGCATCGCCAAACTCAGGTTCTGGCGCCAGCCGTGAAGATGACATGCCCTTCCAAGGGGAAACCAGTGAAG
GCCTGTACGAACATTTAGAATGGCAGAAAAACCTCACGCCCTTCTCTGAAACCGATTTAGCTATTGCCACCGCGATTATT
GATGCCATCGACGATCAAGGTTATCTGACTCAAAGTACTGAAGATATCCTCGAGGCCATGGGCAATCCTGAGATCGAGCT
GGATGAAGTCGAAGCCGTACTCAAGCGCATTCAACACTTCGATCCCGTCGGTGTCGCCGCAAGGGATTTAAGCGAATGTC
TGCTTATCCAACTCTCTCACTTTGCGCTGGATACCCCACACCTCGAAAACGCCCGCATGTTGATCAAAGACCATTTAGAT
CTGATCGCCGCCCGCGACTTTAGATTGTTGATGCGTAAGACCAAACTCAAAGAGGACGAGTTAAGGGAAAGCATAGCGCT
GATCCAAACCTTAAATCCTCGCCCCGGTTTGCTCATCACTCCTGTTGATGAAGAATATGTCATCCCCGATGTATCAGTGT
CGAAGAAAAATGGTCGCTGGGTGGTCGAGTTAAATCCAGACTGCATGCCAAAAATCAATGTTAATCAGCAATATGCAGCC
ATGGCGCGCAGTACCAAAAGCCAAGCCGATAGCCAATTTATCCGCGGGCACCTGCAAGAAGCCAAGTGGTTTATCAAAAG
CCTTGAAAGCCGTAACGAAACACTGCTCAAGGTGGCAAACTGCATAGTGCAATATCAACAGGGCTTCTTCGAATACGGTG
AAGAAGCCATGAAACCTATGGTCCTAAACGATATCGCCGAGGCGGTAGAGATGCATGAGTCCACCATTTCACGGGTGACC
ACACAAAAATACATGCATACCCCAAAGGGACTTTTTGAACTAAAATATTTCTTCTCAAGCCACGTCGGGACTGATGATGG
CGGTGAGTGCTCATCCACGGCAATCAGAGCCTTCATTAAGAAACTGGTTGCAGCGGAAAACCAGAAAAAACCATTAAGCG
ACAGCAAGATGGCGCAACTTCTGGCAGAACAGGGAATTAACGTTGCGCGCAGAACCATTGCGAAGTACCGAGAGGCGATG
CTTATTCCCCCTTCCAATCAGCGTAAAAGTTTATAG

Upstream 100 bases:

>100_bases
TAAACAGAGCCAATTAGCCTTGAATTTTGGCTTATCAGCGCCATCAATATGATAACAAGCGGCCTAAATTCAAAATAATA
ACGTAGGGATCAGCGGTAAC

Downstream 100 bases:

>100_bases
CCTAAAGTGGAAATCGGAGGACGTTTATATGCAAATAAACCTGAGTGGACACCATATCGAGATTACTGAATCGTTACGGG
CTTATGTTGAGGAAAAGTTT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEEQFDAPTERKQDIADTDFSETTAQPQEQD
NSTVDIAESLTKESLPEELPMDTTWDEVFTASPNSGSGASREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAII
DAIDDQGYLTQSTEDILEAMGNPEIELDEVEAVLKRIQHFDPVGVAARDLSECLLIQLSHFALDTPHLENARMLIKDHLD
LIAARDFRLLMRKTKLKEDELRESIALIQTLNPRPGLLITPVDEEYVIPDVSVSKKNGRWVVELNPDCMPKINVNQQYAA
MARSTKSQADSQFIRGHLQEAKWFIKSLESRNETLLKVANCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVT
TQKYMHTPKGLFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREAM
LIPPSNQRKSL

Sequences:

>Translated_491_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEEQFDAPTERKQDIADTDFSETTAQPQEQD
NSTVDIAESLTKESLPEELPMDTTWDEVFTASPNSGSGASREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAII
DAIDDQGYLTQSTEDILEAMGNPEIELDEVEAVLKRIQHFDPVGVAARDLSECLLIQLSHFALDTPHLENARMLIKDHLD
LIAARDFRLLMRKTKLKEDELRESIALIQTLNPRPGLLITPVDEEYVIPDVSVSKKNGRWVVELNPDCMPKINVNQQYAA
MARSTKSQADSQFIRGHLQEAKWFIKSLESRNETLLKVANCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVT
TQKYMHTPKGLFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREAM
LIPPSNQRKSL
>Mature_491_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEEQFDAPTERKQDIADTDFSETTAQPQEQD
NSTVDIAESLTKESLPEELPMDTTWDEVFTASPNSGSGASREDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAII
DAIDDQGYLTQSTEDILEAMGNPEIELDEVEAVLKRIQHFDPVGVAARDLSECLLIQLSHFALDTPHLENARMLIKDHLD
LIAARDFRLLMRKTKLKEDELRESIALIQTLNPRPGLLITPVDEEYVIPDVSVSKKNGRWVVELNPDCMPKINVNQQYAA
MARSTKSQADSQFIRGHLQEAKWFIKSLESRNETLLKVANCIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVT
TQKYMHTPKGLFELKYFFSSHVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREAM
LIPPSNQRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor recognizes a DNA element, designated as region A upstream of the pressure-regulated operon [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=493, Percent_Identity=63.4888438133874, Blast_Score=631, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55388; Mature: 55388

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEEQFDAPTER
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHCCCCCHH
KQDIADTDFSETTAQPQEQDNSTVDIAESLTKESLPEELPMDTTWDEVFTASPNSGSGAS
HHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCC
REDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAIIDAIDDQGYLTQSTEDILEAM
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GNPEIELDEVEAVLKRIQHFDPVGVAARDLSECLLIQLSHFALDTPHLENARMLIKDHLD
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LIAARDFRLLMRKTKLKEDELRESIALIQTLNPRPGLLITPVDEEYVIPDVSVSKKNGRW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCEECCCCEE
VVELNPDCMPKINVNQQYAAMARSTKSQADSQFIRGHLQEAKWFIKSLESRNETLLKVAN
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
CIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPKGLFELKYFFSS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
HVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREAM
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHEE
LIPPSNQRKSL
ECCCCCCCCCC
>Mature Secondary Structure
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDSNPLLELEEEQFDAPTER
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHCCCCCHH
KQDIADTDFSETTAQPQEQDNSTVDIAESLTKESLPEELPMDTTWDEVFTASPNSGSGAS
HHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCC
REDDMPFQGETSEGLYEHLEWQKNLTPFSETDLAIATAIIDAIDDQGYLTQSTEDILEAM
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
GNPEIELDEVEAVLKRIQHFDPVGVAARDLSECLLIQLSHFALDTPHLENARMLIKDHLD
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LIAARDFRLLMRKTKLKEDELRESIALIQTLNPRPGLLITPVDEEYVIPDVSVSKKNGRW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCEECCCCEE
VVELNPDCMPKINVNQQYAAMARSTKSQADSQFIRGHLQEAKWFIKSLESRNETLLKVAN
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
CIVQYQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPKGLFELKYFFSS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
HVGTDDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAQLLAEQGINVARRTIAKYREAM
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHEE
LIPPSNQRKSL
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10760597 [H]