Click here to switch to the map view.

The map label for this gene is mscL [H]

Identifier: 117918974

GI number: 117918974

Start: 611243

End: 611653

Strand: Reverse

Name: mscL [H]

Synonym: Shewana3_0520

Alternate gene names: 117918974

Gene position: 611653-611243 (Counterclockwise)

Preceding gene: 117918975

Following gene: 117918966

Centisome position: 12.3

GC content: 47.69

Gene sequence:

>411_bases
ATGAGCTTAATTAAAGAGTTCAAGGCTTTTGCGTCCCGTGGCAATGTGATCGATATGGCGGTGGGTATTATCATCGGTGC
CGCTTTTGGCAAGATAGTCTCATCCTTTGTAGCCGATATCATTATGCCGCCAATTGGGATCATCTTAGGGGGCGTGAATT
TCAGCGATCTGAGCATAGTGTTGCAAGCCGCCCAGGGCGATGCGCCAGCGGTAGTGATTGCTTACGGTAAATTCATTCAA
ACCATCATCGACTTTACCATTATCGCCTTCGCGATTTTTATGGGCTTAAAAGCCATTAACAGCCTAAAACGCAAACAGGA
AGAAGCGCCGAAAGCCCCACCAGCGCCGACCAAAGATCAAGAGCTGTTATCTGAGATCCGCGATCTGCTTAAAGCGCAGC
AAGAAAAGTAA

Upstream 100 bases:

>100_bases
AGCTTGGCAAGGTAACAGGCAGCAAAAGACCCTTAGGTTTTCCTGATACAGTTAACACAATAAAATTTAACATTTCATTT
TTAATTTCGGAGGAAACCTA

Downstream 100 bases:

>100_bases
GCTAACGCTATTTTTTATCTGATTGATTGCAATAAAAAACCCATCGACTTCGATGGGTTTTGTTTTTATATAAGCGCTTA
GCTTACTCGGGCACGTCGTG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIVLQAAQGDAPAVVIAYGKFIQ
TIIDFTIIAFAIFMGLKAINSLKRKQEEAPKAPPAPTKDQELLSEIRDLLKAQQEK

Sequences:

>Translated_136_residues
MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIVLQAAQGDAPAVVIAYGKFIQ
TIIDFTIIAFAIFMGLKAINSLKRKQEEAPKAPPAPTKDQELLSEIRDLLKAQQEK
>Mature_135_residues
SLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIVLQAAQGDAPAVVIAYGKFIQT
IIDFTIIAFAIFMGLKAINSLKRKQEEAPKAPPAPTKDQELLSEIRDLLKAQQEK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=64.7058823529412, Blast_Score=184, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14559; Mature: 14428

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LQAAQGDAPAVVIAYGKFIQTIIDFTIIAFAIFMGLKAINSLKRKQEEAPKAPPAPTKDQ
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ELLSEIRDLLKAQQEK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LQAAQGDAPAVVIAYGKFIQTIIDFTIIAFAIFMGLKAINSLKRKQEEAPKAPPAPTKDQ
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ELLSEIRDLLKAQQEK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA