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The map label for this gene is dusB [H]

Identifier: 117918856

GI number: 117918856

Start: 457826

End: 458794

Strand: Reverse

Name: dusB [H]

Synonym: Shewana3_0399

Alternate gene names: 117918856

Gene position: 458794-457826 (Counterclockwise)

Preceding gene: 117918857

Following gene: 117918855

Centisome position: 9.23

GC content: 49.64

Gene sequence:

>969_bases
ATGCAGATTGGACCCTATCAACTGAAGAATCAGCTGATCGTGGCACCAATGGCAGGTGTCACCGATCAAGCTTTCCGTAA
TCTCTGTCTTCGTTATGGTGCAGCCTTAGCGGTGTCGGAGATGCTTTCATCGAATCCTGAAGTTTGGGACACAGATAAGA
GCCGCCAGCGTATGACGCATTCCGGCGAGGAAGGTATTCGCTCAGTGCAAATTGCAGGTGCAGATCCAGAGTTAATGGCG
CAGGCCGCCCAGTTCAACGTCGAACAAGGTGCCCACATCATTGATATCAACATGGGATGCCCGGCAAAAAAAGTGAATAA
AAAGCTGGCAGGTTCTGCCCTAATGCAAAATCCACCCTTAGTGAAAGAAATTTTACAAGCCGTGGTCGCTGCAGTAGAGG
TGCCTGTCACGTTAAAGATTCGCACAGGTTGGGAGCCTGAACACAGGAACGGTGTTCAAATCGCCCAGATTGCTGAGGAT
TGTGGTATCGCCTCGCTCGCCGTTCACGGCCGCACGAGACAATGCATGTACAGAGGCGACGCCGAGTACGACACCATCAA
AGCAATTAAACAGAATGTCTCGATTCCTGTTGTCGCGAATGGGGATATCGACAGTCCGGAGAAAGCCCGCTTCGTGCTGG
ACTATACCGGTGTCGATGCTCTGATGATAGGACGGGGTGCTCAAGGACGGCCTTGGATTTTCAGAGAAATCCAGCACTAC
TTGGATACGGGTAATAAGCTAGCGCCCATTGAGGTGGCTGAACAGCGTCAAGTGATGCTGGAACACCTCACCAAACTTTA
TGATTTGTATGGTGAATATAAGGGTATCCGCTTCGCCAGAAAGCATATTGGATGGTACCTAGACCAAGAGGATCAGCGCC
AGTTCCGGGCTGACTTTAATCAGCTGGAAACCGCTGCAGAACAGTATTCCCTGGTGGAATATTATTTTGATGAATTAGTA
CAGAATTAA

Upstream 100 bases:

>100_bases
TCAAGAAAAAAAATTGCTCCTAGGTCATTTATTGACCTTTTCACGGCCAATAAAAAGGGCTACTATAGCGCCCCTTTGAA
GAGCTAAGGTGAAACAGTAA

Downstream 100 bases:

>100_bases
TAAAGAGCAGAATAGAATGTTTGATCAGACAACTAACACAGAAGTTCACCAGCTTACCGTTGGCAAAATCGAAACCGCAA
ACGGCACTATCAAGCCCCAG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQIGPYQLKNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTHSGEEGIRSVQIAGADPELMA
QAAQFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPLVKEILQAVVAAVEVPVTLKIRTGWEPEHRNGVQIAQIAED
CGIASLAVHGRTRQCMYRGDAEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
LDTGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHIGWYLDQEDQRQFRADFNQLETAAEQYSLVEYYFDELV
QN

Sequences:

>Translated_322_residues
MQIGPYQLKNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTHSGEEGIRSVQIAGADPELMA
QAAQFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPLVKEILQAVVAAVEVPVTLKIRTGWEPEHRNGVQIAQIAED
CGIASLAVHGRTRQCMYRGDAEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
LDTGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHIGWYLDQEDQRQFRADFNQLETAAEQYSLVEYYFDELV
QN
>Mature_322_residues
MQIGPYQLKNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTHSGEEGIRSVQIAGADPELMA
QAAQFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPLVKEILQAVVAAVEVPVTLKIRTGWEPEHRNGVQIAQIAED
CGIASLAVHGRTRQCMYRGDAEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
LDTGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHIGWYLDQEDQRQFRADFNQLETAAEQYSLVEYYFDELV
QN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=33.6099585062241, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI239788462, Length=242, Percent_Identity=33.4710743801653, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI31742496, Length=309, Percent_Identity=29.4498381877023, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI40807366, Length=226, Percent_Identity=32.7433628318584, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=32.6530612244898, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=65, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=31.6666666666667, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693211, Length=321, Percent_Identity=27.7258566978193, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=33.0578512396694, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=228, Percent_Identity=30.7017543859649, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=261, Percent_Identity=31.8007662835249, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=29.2181069958848, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=312, Percent_Identity=26.9230769230769, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=27.1929824561404, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585320, Length=291, Percent_Identity=28.1786941580756, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=33.0396475770925, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=33.0396475770925, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=28.9795918367347, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921524, Length=310, Percent_Identity=23.8709677419355, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36124; Mature: 36124

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYQLKNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTH
CCCCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
SGEEGIRSVQIAGADPELMAQAAQFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPL
CCHHCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHH
VKEILQAVVAAVEVPVTLKIRTGWEPEHRNGVQIAQIAEDCGIASLAVHGRTRQCMYRGD
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCHHHHCCCC
AEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
CCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHIGWYLDQEDQRQFRADFN
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QLETAAEQYSLVEYYFDELVQN
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGPYQLKNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTH
CCCCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
SGEEGIRSVQIAGADPELMAQAAQFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPL
CCHHCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHH
VKEILQAVVAAVEVPVTLKIRTGWEPEHRNGVQIAQIAEDCGIASLAVHGRTRQCMYRGD
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCHHHHCCCC
AEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLDYTGVDALMIGRGAQGRPWIFREIQHY
CCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHIGWYLDQEDQRQFRADFN
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QLETAAEQYSLVEYYFDELVQN
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]