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The map label for this gene is pckA [H]

Identifier: 117918605

GI number: 117918605

Start: 170761

End: 172302

Strand: Reverse

Name: pckA [H]

Synonym: Shewana3_0147

Alternate gene names: 117918605

Gene position: 172302-170761 (Counterclockwise)

Preceding gene: 117918606

Following gene: 117918604

Centisome position: 3.47

GC content: 52.85

Gene sequence:

>1542_bases
ATGGCGGATGGATTAAACCGCGTTCACTACAACCCCTCAACAGCACAACTGGTCGAATTTGCCCTGCTACGCGGCGAAGG
TGAATTGACTGCAAACGGCGCACTGGTTGCTAAAACCGGTACCCGCACAGGTCGCTCTCCTGGCGATCGCTTCATTGTAA
AAGAAGACAGTTCAGCGGCTGACATCGAATGGGGCCCCGTCAACCAAGCATTTGAACCCGGTGCTTTTGAAGGATTATGG
GCGCGTGTAGAAGCATTCCTCGCCGATAAAGAGTTGTTTGTATCTGACCTCGAAGTCGGTGCAGACCCAGAACATTACCA
GCCAGTGCGTGTGACGACTCAATACGCTTGGCACCAACTGTTCGCCCGCAACCTTTTTATCATCCCAGAAGAATTTAACC
GTCAAGACAAGCCTGTATGGCAAATCATCAACGCGCCAGACTTCGTCTGTGTTCCTGAGCGTGATGGCACCAATTCAGAT
GCAGCGGTGATCTTAAACTTTGCCGAGCGCAAAGTGTTGCTAGCGGGCCTGAAATACGCTGGCGAAATGAAGAAGTCGAT
GTTCTCTGTACAAAACTTCCTGCTGCCAGCCCAAGGCGTATTGCCAATGCACTGCTCGGCAAACGTAGGTAAAGACGGCG
ACACTACCCTATTCTTCGGTCTGTCAGGCACGGGTAAAACCACCCTGTCTGCGGATCCAAAACGCTTCCTGATCGGTGAC
GATGAGCACGGCTGGGCACCAGGCGGCGTATTCAACATCGAAGGCGGCTGCTACGCCAAGTGTATCGATCTGAGCCAAAA
GAACGAGCCAGTGATTTGGGATGCGATTCGCTTCGGTACTGTGCTGGAAAACGTGGTAATGGACGAGCACCGCGTGCCTA
ACTACAAAGACAGCAGCCTGACGGAAAACACCCGTGCAGCTTACCCACTCGAGCATATCGCTCAGCGTAAAGAAGACAAC
CGTGGCGCCGAGCCGCACGCAGTGGTGTTCTTAACCTGCGACGTGTCTGGCGTATTACCACCGGTTTCTATCCTGAGCAA
AGAGCAAGCGGCTTACCACTTCCTCTCTGGTTACACCGCTAAAGTCGGTTCGACTGAAATCGGTTCAACCTCAGCGATTC
AGTCAACCTTCTCTACTTGCTTCGGCGCGCCTTTCTTCCCACGTCCTGCGGGCGTGTATGCAGAGCTGCTGATGAAGCGT
ATCGAATCATTCGGTAGCCAAGTGTACTTAGTTAACACTGGCTGGACTGGCGGTCCACACGGTATTGGTAAACGTTTCGA
TATCCCAACGACGCGCGCAATTGTCGATGCGATTGTCAGCGGTGAGCTGAAAAACGTCGAAACCGTACACTTAGAGACAC
TGAATCTGGCCGTGCCTGTCGCGGTACCCGGTGTAGACACTAACCTACTCAACCCAGTGAACACTTGGAGCGATAAGGCA
CTCTACGCCGAATACGCCCAAAAACTGGCCGAAGCCTTCACTAAGAACTTCGCTAAGTACCAAGTGTCAGATGCCATCCG
AAACGCTGGCCCAAAGGCATAA

Upstream 100 bases:

>100_bases
TTTGTCCCCTAGCACGCCTCGGGCGATAAATAAAAGCAAAATCGGCAGACGATCGATTAAAAAAATAACCTTACAAATAC
AAGTCAATGGAGACCTCACT

Downstream 100 bases:

>100_bases
GCGGTTAGCGGACTCATTTAGATAATGTAATCAAACACATTGTTGAGTAAAAAGCGCGGGTTCAGCGATGAACCCGCGCT
TTTTATTTGCCTGTTGATTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MADGLNRVHYNPSTAQLVEFALLRGEGELTANGALVAKTGTRTGRSPGDRFIVKEDSSAADIEWGPVNQAFEPGAFEGLW
ARVEAFLADKELFVSDLEVGADPEHYQPVRVTTQYAWHQLFARNLFIIPEEFNRQDKPVWQIINAPDFVCVPERDGTNSD
AAVILNFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSLTENTRAAYPLEHIAQRKEDN
RGAEPHAVVFLTCDVSGVLPPVSILSKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKNVETVHLETLNLAVPVAVPGVDTNLLNPVNTWSDKA
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA

Sequences:

>Translated_513_residues
MADGLNRVHYNPSTAQLVEFALLRGEGELTANGALVAKTGTRTGRSPGDRFIVKEDSSAADIEWGPVNQAFEPGAFEGLW
ARVEAFLADKELFVSDLEVGADPEHYQPVRVTTQYAWHQLFARNLFIIPEEFNRQDKPVWQIINAPDFVCVPERDGTNSD
AAVILNFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSLTENTRAAYPLEHIAQRKEDN
RGAEPHAVVFLTCDVSGVLPPVSILSKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKR
IESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKNVETVHLETLNLAVPVAVPGVDTNLLNPVNTWSDKA
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA
>Mature_512_residues
ADGLNRVHYNPSTAQLVEFALLRGEGELTANGALVAKTGTRTGRSPGDRFIVKEDSSAADIEWGPVNQAFEPGAFEGLWA
RVEAFLADKELFVSDLEVGADPEHYQPVRVTTQYAWHQLFARNLFIIPEEFNRQDKPVWQIINAPDFVCVPERDGTNSDA
AVILNFAERKVLLAGLKYAGEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGDD
EHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSLTENTRAAYPLEHIAQRKEDNR
GAEPHAVVFLTCDVSGVLPPVSILSKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRI
ESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKNVETVHLETLNLAVPVAVPGVDTNLLNPVNTWSDKAL
YAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=533, Percent_Identity=46.3414634146341, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322950, Length=523, Percent_Identity=43.9770554493308, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 55983; Mature: 55851

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGLNRVHYNPSTAQLVEFALLRGEGELTANGALVAKTGTRTGRSPGDRFIVKEDSSAA
CCCCCCCEECCCCHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCEEEEECCCCCC
DIEWGPVNQAFEPGAFEGLWARVEAFLADKELFVSDLEVGADPEHYQPVRVTTQYAWHQL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHH
FARNLFIIPEEFNRQDKPVWQIINAPDFVCVPERDGTNSDAAVILNFAERKVLLAGLKYA
HHCCEEEECHHCCCCCCCCEEEECCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHH
GEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
TENTRAAYPLEHIAQRKEDNRGAEPHAVVFLTCDVSGVLPPVSILSKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPH
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GIGKRFDIPTTRAIVDAIVSGELKNVETVHLETLNLAVPVAVPGVDTNLLNPVNTWSDKA
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCHH
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADGLNRVHYNPSTAQLVEFALLRGEGELTANGALVAKTGTRTGRSPGDRFIVKEDSSAA
CCCCCCEECCCCHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCEEEEECCCCCC
DIEWGPVNQAFEPGAFEGLWARVEAFLADKELFVSDLEVGADPEHYQPVRVTTQYAWHQL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHH
FARNLFIIPEEFNRQDKPVWQIINAPDFVCVPERDGTNSDAAVILNFAERKVLLAGLKYA
HHCCEEEECHHCCCCCCCCEEEECCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHH
GEMKKSMFSVQNFLLPAQGVLPMHCSANVGKDGDTTLFFGLSGTGKTTLSADPKRFLIGD
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCEEEECC
DEHGWAPGGVFNIEGGCYAKCIDLSQKNEPVIWDAIRFGTVLENVVMDEHRVPNYKDSSL
CCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
TENTRAAYPLEHIAQRKEDNRGAEPHAVVFLTCDVSGVLPPVSILSKEQAAYHFLSGYTA
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPH
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GIGKRFDIPTTRAIVDAIVSGELKNVETVHLETLNLAVPVAVPGVDTNLLNPVNTWSDKA
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCHH
LYAEYAQKLAEAFTKNFAKYQVSDAIRNAGPKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA