Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 117918576

GI number: 117918576

Start: 134067

End: 134690

Strand: Reverse

Name: rhtB [H]

Synonym: Shewana3_0118

Alternate gene names: 117918576

Gene position: 134690-134067 (Counterclockwise)

Preceding gene: 117918577

Following gene: 117918573

Centisome position: 2.71

GC content: 51.44

Gene sequence:

>624_bases
ATGCAGACCGAAACTTGGTTACTCTACCTTTTTGCGATTGTGTTGATTGGGATTTCCCCCGGGCCTATCGCCATGTTGTC
TATGTCCCACGGCATTCATTTCGGTAAAAAACGCAGCATAGCCACAGGGCTTGGCAGTGTCAGCGCCGCGCTAGTGCTGA
TGGCGGCTTCGGCGGCAGGATTAGGCGCGATAATCAGCGCCTCGGAATACGGCTTTAGCCTACTCAAATGGTGCGGCGCC
GCCTATCTGGTGTTTTTGGGCATTAAGTTATTGCTCACTAAGCAACAAGGGCAAAAGCTGGAAGTAACGAAAACCGAAGG
AAAAGGGACAGCGAAGCAACTCTATAAGCAGGCGTTTTTAGTGGGGATCAGCAATCCTAAAGACTTACTGTTTTTTGCGG
CGTTATTTCCACAATTTATCGATTTAACCGCACCACAAATGCCGCAACTGACGATTCTTGCCCTCACCTGGGCGCTGGTC
GATTTTAGCTTTGTGATGCTGTACGCCTCGATGGCGAATGTGCTGGCCCCGAGCCTAAAGGCCAGCAACAAACTGCATTG
GTTTGACCGTACCAGCGGCGGCGTATTTCTCACCCTCGCCGCCATCTTAGTCAGTCGGGATTAG

Upstream 100 bases:

>100_bases
CTCAGTTTTTACCGCAGCATTAAAGGGCGTCATCCCAAAACGATCCCCGACTATACGCCAGACAACAGACGTTAACGCCT
GAGGAATCACGGAGCAAAAC

Downstream 100 bases:

>100_bases
ATTAGCCCTCGACTTTCACCGATTTTAGACTGCGGATATCGTTGCCTGTCGGCGCTTGGAAGGCCTGTAAACCAAACTCG
GGCAATACGGCAAAGATATG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQTETWLLYLFAIVLIGISPGPIAMLSMSHGIHFGKKRSIATGLGSVSAALVLMAASAAGLGAIISASEYGFSLLKWCGA
AYLVFLGIKLLLTKQQGQKLEVTKTEGKGTAKQLYKQAFLVGISNPKDLLFFAALFPQFIDLTAPQMPQLTILALTWALV
DFSFVMLYASMANVLAPSLKASNKLHWFDRTSGGVFLTLAAILVSRD

Sequences:

>Translated_207_residues
MQTETWLLYLFAIVLIGISPGPIAMLSMSHGIHFGKKRSIATGLGSVSAALVLMAASAAGLGAIISASEYGFSLLKWCGA
AYLVFLGIKLLLTKQQGQKLEVTKTEGKGTAKQLYKQAFLVGISNPKDLLFFAALFPQFIDLTAPQMPQLTILALTWALV
DFSFVMLYASMANVLAPSLKASNKLHWFDRTSGGVFLTLAAILVSRD
>Mature_207_residues
MQTETWLLYLFAIVLIGISPGPIAMLSMSHGIHFGKKRSIATGLGSVSAALVLMAASAAGLGAIISASEYGFSLLKWCGA
AYLVFLGIKLLLTKQQGQKLEVTKTEGKGTAKQLYKQAFLVGISNPKDLLFFAALFPQFIDLTAPQMPQLTILALTWALV
DFSFVMLYASMANVLAPSLKASNKLHWFDRTSGGVFLTLAAILVSRD

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=205, Percent_Identity=30.2439024390244, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788099, Length=158, Percent_Identity=31.0126582278481, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22248; Mature: 22248

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTETWLLYLFAIVLIGISPGPIAMLSMSHGIHFGKKRSIATGLGSVSAALVLMAASAAG
CCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LGAIISASEYGFSLLKWCGAAYLVFLGIKLLLTKQQGQKLEVTKTEGKGTAKQLYKQAFL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
VGISNPKDLLFFAALFPQFIDLTAPQMPQLTILALTWALVDFSFVMLYASMANVLAPSLK
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNKLHWFDRTSGGVFLTLAAILVSRD
CCCCEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQTETWLLYLFAIVLIGISPGPIAMLSMSHGIHFGKKRSIATGLGSVSAALVLMAASAAG
CCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LGAIISASEYGFSLLKWCGAAYLVFLGIKLLLTKQQGQKLEVTKTEGKGTAKQLYKQAFL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
VGISNPKDLLFFAALFPQFIDLTAPQMPQLTILALTWALVDFSFVMLYASMANVLAPSLK
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNKLHWFDRTSGGVFLTLAAILVSRD
CCCCEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]