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The map label for this gene is glyS [H]

Identifier: 117918475

GI number: 117918475

Start: 18813

End: 20879

Strand: Reverse

Name: glyS [H]

Synonym: Shewana3_0016

Alternate gene names: 117918475

Gene position: 20879-18813 (Counterclockwise)

Preceding gene: 117918476

Following gene: 117918472

Centisome position: 0.42

GC content: 48.62

Gene sequence:

>2067_bases
ATGAATTTTGAAAACTTACTCATCGAGTTAGGCACTGAAGAACTGCCGCCAAAGGCGCTGCGTAAACTGGCTGAGTCTTT
CTTAGCGAATTTTACCGAAGAATTAACCAAAGCCGATTTAGCTTTTAAATCAGCTGTTTGGTATGCAGCACCCCGTCGTC
TCGCCATTAATGTGACAGAACTAGCACTTGCTCAAGCGGACAAAATCGTTGAAAAACGCGGCCCAGCTGTCAGCTCAGCA
TTCGATGCCGAAGGTAAACCAACGAAAGCCGCAGAAGGTTGGGCACGTGGTAACGGTATTACCGTTGACCAAGCCGAGCG
TTTAGTGACCGATAAAGGTGAATGGCTGGTTTATAACGCCAAAGTCGAAGGCGTTGAAACCAAAAGCTTAATCGCTGCGA
TGGCGCAACGTGCGCTGGATAAGTTACCCATTCCTAAGCCAATGCGCTGGGGAAGCAGCAAGACACAATTCATTCGCCCT
GTTCACACAGCCACTATGCTGTTAGGCAGTGAGTTAATCGAAGGTGAATTACTTGGCATTAAATCGGCACGTAATGTTCG
TGGTCACCGCTTTATGGGTACAGGCTTCGAGCTTGACCATGCAGACAACTACCTGACGCTACTGAAAGAAAAAGGTAAAG
TCATCGCCGATTACGAAAGCCGTAAAGCCTTAATTAAAGCCGATGCAGAAAAAGCGGCCGCTAAGATTGGTGGCACCGCA
GATATCGAAGATGACCTGCTGGAAGAAGTCACCTCTTTGGTTGAATGGCCAGTCGTATTAACCGCAAGCTTTGAAGAGAA
GTTCTTGAACGTGCCTTCAGAAGCCTTGGTTTATACGATGAAGGGCGATCAGAAATACTTCCCAGTGTTCGATGATGCAG
GTAAGTTACTGCCAAACTTCATCTTTGTCGCTAACATAGAATCAAAAGATCCGGCACAAATTATTGCGGGTAACGAGAAG
GTGGTTCGTCCACGCTTGGCCGATGCCGAGTTCTTCTTCAATACCGACAAAAAGCACACGCTGGAATCACGCTTACCGAG
TCTTGAGACCGTCTTGTTCCAACAACAACTAGGTACTTTAAAAGACAAAGTCACTCGTATCTCTGCCCTAGCCGCCTTTA
TTGCCGAGCAAACTGGCGCTAACGCCGTTGATGCAGCCCGTGCTGGTTTATTGTCTAAAACAGACTTAATGACCAATATG
GTGATGGAGTTTACCGATACTCAAGGCACTATGGGCATGCATTACGCCCGCCTAGATGGTGAAACTGAAGCCGTCGCCTT
AGCCATGGAAGAGCAATACAAGCCAAAATTCTCTGGCGATACAGTTCCTACTGCAGCGGTATCTTGCGCAGTAGCCTTAG
CGGATAAACTCGATACCTTAGTCGGTATCTTCGGTATTGGTCAGGCACCTAAGGGCGCTGCCGACCCATTCGCACTGCGC
CGCGCCGCTATCGGTGTGCTACGCATTATCGTTGAGAACAAGTTGCCACTAGACTTAGTGACCCTGATTGCCAAGGCTCA
AGAACTCCATGGCACTAACTTAAGCAACGCGAATGCAAGCGATGAAGTGCTTGAGTTCCTAATGGCGCGTTTCCGCGCTT
GGTACCAAGATAAAGGTATCGAGGTTGACGTGATCCTCGCCGTATTAGCACGTCGCCCAACTCGCCCAGCTGATTTTGAT
AGCCGTATCAATGCGGTATCACACTTCAGAAGCTTAGAAGCCTCTAGCGCTCTGGCAGCGGCGAATAAGCGTGTGTCTAA
CATTCTGGCGAAAGTGGAAGGCGAATTACCCACCACGATTAACTCAGCTCTGTTAGCCGAAGCCGCAGAACAAGCATTAG
CCGCTAAGCTTGCCGAACTGCAGCCTCAACTCGCTCCGCTGTTCGCTAATGCTGATTACCAACAGGCATTAACACTGCTG
GCGAGCTTACGTGAAAGCGTTGACCAGTTCTTCGAAGACGTGATGGTTATGGCGGACGACGAAGCATTGAAAAACAACCG
CTTAGCTCTGCTAAATAACCTACGTGAACAGTTCTTACATGTAGCGGATATTTCTCTGCTTCAGTAA

Upstream 100 bases:

>100_bases
TTATATCCTGCGCGTTCGTACCATGGCTAAAGCCGTTGCTGAATCCTATTATCAGGCACGCGAAGCGCTTGGCTTCCCAA
TGTGTAAGTAGAGGTAACAC

Downstream 100 bases:

>100_bases
GTTGCACGTTAGAGTGCCAATAAAAAACGCGCTTATAGCGCGTTTTTTATTGGCTGCAATATATTAAAACTGCTGAAGTT
TTTCGTGCAACGCTTCGAAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MNFENLLIELGTEELPPKALRKLAESFLANFTEELTKADLAFKSAVWYAAPRRLAINVTELALAQADKIVEKRGPAVSSA
FDAEGKPTKAAEGWARGNGITVDQAERLVTDKGEWLVYNAKVEGVETKSLIAAMAQRALDKLPIPKPMRWGSSKTQFIRP
VHTATMLLGSELIEGELLGIKSARNVRGHRFMGTGFELDHADNYLTLLKEKGKVIADYESRKALIKADAEKAAAKIGGTA
DIEDDLLEEVTSLVEWPVVLTASFEEKFLNVPSEALVYTMKGDQKYFPVFDDAGKLLPNFIFVANIESKDPAQIIAGNEK
VVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTLKDKVTRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNM
VMEFTDTQGTMGMHYARLDGETEAVALAMEEQYKPKFSGDTVPTAAVSCAVALADKLDTLVGIFGIGQAPKGAADPFALR
RAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANASDEVLEFLMARFRAWYQDKGIEVDVILAVLARRPTRPADFD
SRINAVSHFRSLEASSALAAANKRVSNILAKVEGELPTTINSALLAEAAEQALAAKLAELQPQLAPLFANADYQQALTLL
ASLRESVDQFFEDVMVMADDEALKNNRLALLNNLREQFLHVADISLLQ

Sequences:

>Translated_688_residues
MNFENLLIELGTEELPPKALRKLAESFLANFTEELTKADLAFKSAVWYAAPRRLAINVTELALAQADKIVEKRGPAVSSA
FDAEGKPTKAAEGWARGNGITVDQAERLVTDKGEWLVYNAKVEGVETKSLIAAMAQRALDKLPIPKPMRWGSSKTQFIRP
VHTATMLLGSELIEGELLGIKSARNVRGHRFMGTGFELDHADNYLTLLKEKGKVIADYESRKALIKADAEKAAAKIGGTA
DIEDDLLEEVTSLVEWPVVLTASFEEKFLNVPSEALVYTMKGDQKYFPVFDDAGKLLPNFIFVANIESKDPAQIIAGNEK
VVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTLKDKVTRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNM
VMEFTDTQGTMGMHYARLDGETEAVALAMEEQYKPKFSGDTVPTAAVSCAVALADKLDTLVGIFGIGQAPKGAADPFALR
RAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANASDEVLEFLMARFRAWYQDKGIEVDVILAVLARRPTRPADFD
SRINAVSHFRSLEASSALAAANKRVSNILAKVEGELPTTINSALLAEAAEQALAAKLAELQPQLAPLFANADYQQALTLL
ASLRESVDQFFEDVMVMADDEALKNNRLALLNNLREQFLHVADISLLQ
>Mature_688_residues
MNFENLLIELGTEELPPKALRKLAESFLANFTEELTKADLAFKSAVWYAAPRRLAINVTELALAQADKIVEKRGPAVSSA
FDAEGKPTKAAEGWARGNGITVDQAERLVTDKGEWLVYNAKVEGVETKSLIAAMAQRALDKLPIPKPMRWGSSKTQFIRP
VHTATMLLGSELIEGELLGIKSARNVRGHRFMGTGFELDHADNYLTLLKEKGKVIADYESRKALIKADAEKAAAKIGGTA
DIEDDLLEEVTSLVEWPVVLTASFEEKFLNVPSEALVYTMKGDQKYFPVFDDAGKLLPNFIFVANIESKDPAQIIAGNEK
VVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTLKDKVTRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNM
VMEFTDTQGTMGMHYARLDGETEAVALAMEEQYKPKFSGDTVPTAAVSCAVALADKLDTLVGIFGIGQAPKGAADPFALR
RAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANASDEVLEFLMARFRAWYQDKGIEVDVILAVLARRPTRPADFD
SRINAVSHFRSLEASSALAAANKRVSNILAKVEGELPTTINSALLAEAAEQALAAKLAELQPQLAPLFANADYQQALTLL
ASLRESVDQFFEDVMVMADDEALKNNRLALLNNLREQFLHVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=67.0537010159652, Blast_Score=925, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75155; Mature: 75155

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFENLLIELGTEELPPKALRKLAESFLANFTEELTKADLAFKSAVWYAAPRRLAINVTE
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
LALAQADKIVEKRGPAVSSAFDAEGKPTKAAEGWARGNGITVDQAERLVTDKGEWLVYNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
KVEGVETKSLIAAMAQRALDKLPIPKPMRWGSSKTQFIRPVHTATMLLGSELIEGELLGI
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
KSARNVRGHRFMGTGFELDHADNYLTLLKEKGKVIADYESRKALIKADAEKAAAKIGGTA
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCC
DIEDDLLEEVTSLVEWPVVLTASFEEKFLNVPSEALVYTMKGDQKYFPVFDDAGKLLPNF
CCHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCHHEEEEECCCCEECCCCCCHHHHCCCE
IFVANIESKDPAQIIAGNEKVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTL
EEEEECCCCCCCCEEECCCCEECCCCCCCHHEECCCCHHHHHHHCCHHHHHHHHHHHHHH
KDKVTRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNMVMEFTDTQGTMGMHYARLDG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
ETEAVALAMEEQYKPKFSGDTVPTAAVSCAVALADKLDTLVGIFGIGQAPKGAADPFALR
CCHHEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANASDEVLEFLMARFRAWYQDKGI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
EVDVILAVLARRPTRPADFDSRINAVSHFRSLEASSALAAANKRVSNILAKVEGELPTTI
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSALLAEAAEQALAAKLAELQPQLAPLFANADYQQALTLLASLRESVDQFFEDVMVMADD
HHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EALKNNRLALLNNLREQFLHVADISLLQ
HHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFENLLIELGTEELPPKALRKLAESFLANFTEELTKADLAFKSAVWYAAPRRLAINVTE
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
LALAQADKIVEKRGPAVSSAFDAEGKPTKAAEGWARGNGITVDQAERLVTDKGEWLVYNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
KVEGVETKSLIAAMAQRALDKLPIPKPMRWGSSKTQFIRPVHTATMLLGSELIEGELLGI
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
KSARNVRGHRFMGTGFELDHADNYLTLLKEKGKVIADYESRKALIKADAEKAAAKIGGTA
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCC
DIEDDLLEEVTSLVEWPVVLTASFEEKFLNVPSEALVYTMKGDQKYFPVFDDAGKLLPNF
CCHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCHHEEEEECCCCEECCCCCCHHHHCCCE
IFVANIESKDPAQIIAGNEKVVRPRLADAEFFFNTDKKHTLESRLPSLETVLFQQQLGTL
EEEEECCCCCCCCEEECCCCEECCCCCCCHHEECCCCHHHHHHHCCHHHHHHHHHHHHHH
KDKVTRISALAAFIAEQTGANAVDAARAGLLSKTDLMTNMVMEFTDTQGTMGMHYARLDG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
ETEAVALAMEEQYKPKFSGDTVPTAAVSCAVALADKLDTLVGIFGIGQAPKGAADPFALR
CCHHEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
RAAIGVLRIIVENKLPLDLVTLIAKAQELHGTNLSNANASDEVLEFLMARFRAWYQDKGI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
EVDVILAVLARRPTRPADFDSRINAVSHFRSLEASSALAAANKRVSNILAKVEGELPTTI
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSALLAEAAEQALAAKLAELQPQLAPLFANADYQQALTLLASLRESVDQFFEDVMVMADD
HHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EALKNNRLALLNNLREQFLHVADISLLQ
HHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA