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The map label for this gene is insE1 [H]

Identifier: 117676099

GI number: 117676099

Start: 21137

End: 21307

Strand: Direct

Name: insE1 [H]

Synonym: Shewana3_4159

Alternate gene names: 117676099

Gene position: 21137-21307 (Clockwise)

Preceding gene: 117676098

Following gene: 117676100

Centisome position: 7.58

GC content: 42.69

Gene sequence:

>171_bases
ATGTCAACGAAGAAACCCCGTAAAATCCACACGCAAGAATTTAAAGCTGAAGCCCTGAAGCTTGCTGATAAAATTGGTGT
AGCGGCAGCGGCACGTGAACTCAATCTTTATGAGTCACAGCTATACAATTGGCGCTCAAGTGTCGAAAAGAAATCTAATC
TGAACCAGTAA

Upstream 100 bases:

>100_bases
AGCGCTAGCAGCAAGTAGGTGGTGGCTGAAAAGAATTTGATCTGATCCGCTACTTTTGGACATCTCGCTAAGTGAGTAAA
ATCGCTTATTGAGGTGAAGT

Downstream 100 bases:

>100_bases
TAGTGGACACTTTGTATTATTACTAAAATTTGGTCAATTAGCCTAAGGGAACTGGTGACAGCCTCAAGAGTTGTGCCTCT
GAATGGGGCACGAACGTGTC

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 56; Mature: 55

Protein sequence:

>56_residues
MSTKKPRKIHTQEFKAEALKLADKIGVAAAARELNLYESQLYNWRSSVEKKSNLNQ

Sequences:

>Translated_56_residues
MSTKKPRKIHTQEFKAEALKLADKIGVAAAARELNLYESQLYNWRSSVEKKSNLNQ
>Mature_55_residues
STKKPRKIHTQEFKAEALKLADKIGVAAAARELNLYESQLYNWRSSVEKKSNLNQ

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2963

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 8 family [H]

Homologues:

Organism=Escherichia coli, GI226510958, Length=45, Percent_Identity=75.5555555555556, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI226510933, Length=45, Percent_Identity=75.5555555555556, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI226510930, Length=45, Percent_Identity=75.5555555555556, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI226510928, Length=45, Percent_Identity=75.5555555555556, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI226510927, Length=45, Percent_Identity=75.5555555555556, Blast_Score=77, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002514 [H]

Pfam domain/function: PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 6434; Mature: 6303

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKKPRKIHTQEFKAEALKLADKIGVAAAARELNLYESQLYNWRSSVEKKSNLNQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STKKPRKIHTQEFKAEALKLADKIGVAAAARELNLYESQLYNWRSSVEKKSNLNQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]