Click here to switch to the map view.

The map label for this gene is gshAB

Identifier: 116874133

GI number: 116874133

Start: 2751179

End: 2753509

Strand: Direct

Name: gshAB

Synonym: lwe2717

Alternate gene names: 116874133

Gene position: 2751179-2753509 (Clockwise)

Preceding gene: 116874128

Following gene: 116874137

Centisome position: 97.76

GC content: 37.37

Gene sequence:

>2331_bases
ATGATAAAACTTGATATGACAATACTTGATTCTCTTAAAGAAAACAAAGCTCTCCGGAAACTTTTATTTTCTGGTCATTT
TGGTTTGGAAAAAGAAAATATCCGTGTAACTTCTGATGGAAAACTGGCTCTTACGCCTCATCCAGCTATTTTTGGACCAA
AAGAAGATAATCCATACATTAAAACTGATTTTTCGGAAAGCCAAATTGAAATGATTACTCCAGTAACCGATTCGATTGAC
GATGTATATAACTGGCTTGAAAACCTCCATAATATTGTTTCTTTACGTTCCAAAAATGAACTTCTTTGGCCTTCTAGCAA
TCCGCCAATTTTGCCTGCGGAGAAGGACATTCCTATTGCTGAATATAAAACGCCAGACAGTCCAGATAGAAAATATCGCG
AACATTTAGCGCAAGGTTATGGTAAAAAAATCCAATTATTATCTGGAATTCACTATAATTTTTCATTCCCAGAAGCGTTA
ATTGATGGGCTTTATGATGAGATCAGTCTTCCTAATGAATCGAAGCGCGACTTTAAAAATCGCTTATACTTAAAAGTAGC
TAAATATTTTATGAAAAATCGTTGGTTGCTCATTTATTTAACTGGTGCAAGTCCTGTTTATCTTGCTGATTTCACAAAAA
CCAAACAGGAAGAGAAACTTCGCGATGGTAGCAGCGCTCTTCACGACGGAATTTCCCTTCGTAACAGTAATGCTGGATAT
AAAAATAAAGAATCACTTTACGTTGATTACAATTCATTTGATGCTTACATTTCGAGTATCTCAAACTACATTGAAGCGGG
TAAAATTGAAAGTATGCGCGAATTTTATAACCCGATAAGATTAAAAAACGCACATACTGACCAAACTGTTGAAAGTTTAG
CGAAGCATGGCGTGGAATATTTAGAAATTCGTTCCATTGATTTAAATCCACTTGAACCAAATGGAATTTCTAAAGAGGCG
CTTCATTTTATTCACCTATTCTTAATCAAAGGTTTACTGTCAGAAGACCGCGAGCTATGCGAAAACAACCAACAGTTAGC
TGATGAAAACGAAAATAATATTGCACTAAATGGTCTTTCGAAACCAGCAATTAAAAACTGTGACAACGAAGAAATGGCCC
TTGCAGACGCTGGACTCTTGGAATTAGATAAAATGAACGATTTCATCCAAAGCTTACGTCCAGAAGACACCTATTTCCAA
GCAATCATCGAAAAACAAAAAGAACGCCTGCTACACCCTGAAAAAACGATTGCGGCACAAGTGAAAGAACAATCAGCAAC
AGCAGGTTTTATCGAATTCCATTTAAATCAAGCAAAAACTTACATGGAAGAAACCGAAGCACTAGCTTATAAACTAATTG
GTGCAGAAGACATGGAACTTTCCACACAAATTATTTGGAAAGATGCAATCGCTCGCGGTATCAAAGTTGATGTATTAGAC
CGAGCTGAAAACTTCCTTCGCTTCCAAAAAGGTGACCATGTAGAATATGTGAAACAAGCCAGTAAAACTTCTAAAGACAA
CTATGTTTCCGTTTTAATGATGGAAAATAAAGTGGTGACGAAGCTTGTACTAGCAGAGAACAACATTCGCGTGCCATTCG
GCGATAGTTTTAGTGACCAAGCACTAGCGCTTGAAGCATTTTCCTTATTTAAAGATAAACAAATCGTCGTTAAACCTAAG
TCCACCAACTACGGATGGGGAATCAGTATTTTTAAAAATAAATTCACGACAGAAGATTACCAAGAAGCATTAAATATCGC
TTTTAGTTATGATAGTTCTGTGATTATTGAAGAATTCATTCCCGGAGACGAATTCCGCTTTTTAGTAATTAATGACAAAG
TAGAAGCCGTGCTAAAACGCGTTCCTGCTAATGTCACAGGTGATGGAATTCATACTGTCCGCGAATTAGTTGAAGAAAAA
AATATGGATCCGTTGCGCGGAACCGACCATTTAAAACCACTTGAAAAAATTCGTACTGGCCCAGAAGAAACACTCATGCT
TTCCATGCAAAAACTTTCTTGGGATAGCATTCCAAAAGCCAATGAGACCATCTACCTTCGCGAAAACTCCAATGTCAGCA
CTGGTGGCGATAGCATTGACTATACGGCGGAAATGGACGATTACTTCAAAGAAATAGCCATTCGCGCTACACAGGTTCTC
GATGCCAAAATTTGCGGAGTAGACATAATTGTTCCACGTGAAACAATTGACCGAGATAAACACGCTATCATTGAGCTAAA
CTTTAACCCCGCTATGCATATGCACTGCTTCCCTTATCAAGGTGAGCAGAAAAAAATTGGGGATAAGATTTTAGATTTCT
TATTTGAATAA

Upstream 100 bases:

>100_bases
TCTTTGCAACTGTAAATCACAAACCAATCATTATACTAATTTCACATATTGCGGTAAAATATTTTTATATGAACTTATTT
AAAACCCCTGAGGTGAAAAC

Downstream 100 bases:

>100_bases
AAAAATCCCAGCTACTTCATTAGTAGTTGGGATTTTTTCTTTACTCCTCTAATCCATTTTCACCATTCGAGCTAATAACA
TCTTTATACCAATAATAAGA

Product: bifunctional glutamate--cysteine ligase/glutathione synthetase

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MIKLDMTILDSLKENKALRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSID
DVYNWLENLHNIVSLRSKNELLWPSSNPPILPAEKDIPIAEYKTPDSPDRKYREHLAQGYGKKIQLLSGIHYNFSFPEAL
IDGLYDEISLPNESKRDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFTKTKQEEKLRDGSSALHDGISLRNSNAGY
KNKESLYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAKHGVEYLEIRSIDLNPLEPNGISKEA
LHFIHLFLIKGLLSEDRELCENNQQLADENENNIALNGLSKPAIKNCDNEEMALADAGLLELDKMNDFIQSLRPEDTYFQ
AIIEKQKERLLHPEKTIAAQVKEQSATAGFIEFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAENNIRVPFGDSFSDQALALEAFSLFKDKQIVVKPK
STNYGWGISIFKNKFTTEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEK
NMDPLRGTDHLKPLEKIRTGPEETLMLSMQKLSWDSIPKANETIYLRENSNVSTGGDSIDYTAEMDDYFKEIAIRATQVL
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEQKKIGDKILDFLFE

Sequences:

>Translated_776_residues
MIKLDMTILDSLKENKALRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSID
DVYNWLENLHNIVSLRSKNELLWPSSNPPILPAEKDIPIAEYKTPDSPDRKYREHLAQGYGKKIQLLSGIHYNFSFPEAL
IDGLYDEISLPNESKRDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFTKTKQEEKLRDGSSALHDGISLRNSNAGY
KNKESLYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAKHGVEYLEIRSIDLNPLEPNGISKEA
LHFIHLFLIKGLLSEDRELCENNQQLADENENNIALNGLSKPAIKNCDNEEMALADAGLLELDKMNDFIQSLRPEDTYFQ
AIIEKQKERLLHPEKTIAAQVKEQSATAGFIEFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAENNIRVPFGDSFSDQALALEAFSLFKDKQIVVKPK
STNYGWGISIFKNKFTTEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEK
NMDPLRGTDHLKPLEKIRTGPEETLMLSMQKLSWDSIPKANETIYLRENSNVSTGGDSIDYTAEMDDYFKEIAIRATQVL
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEQKKIGDKILDFLFE
>Mature_776_residues
MIKLDMTILDSLKENKALRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSID
DVYNWLENLHNIVSLRSKNELLWPSSNPPILPAEKDIPIAEYKTPDSPDRKYREHLAQGYGKKIQLLSGIHYNFSFPEAL
IDGLYDEISLPNESKRDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFTKTKQEEKLRDGSSALHDGISLRNSNAGY
KNKESLYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAKHGVEYLEIRSIDLNPLEPNGISKEA
LHFIHLFLIKGLLSEDRELCENNQQLADENENNIALNGLSKPAIKNCDNEEMALADAGLLELDKMNDFIQSLRPEDTYFQ
AIIEKQKERLLHPEKTIAAQVKEQSATAGFIEFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAENNIRVPFGDSFSDQALALEAFSLFKDKQIVVKPK
STNYGWGISIFKNKFTTEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEK
NMDPLRGTDHLKPLEKIRTGPEETLMLSMQKLSWDSIPKANETIYLRENSNVSTGGDSIDYTAEMDDYFKEIAIRATQVL
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEQKKIGDKILDFLFE

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789044, Length=434, Percent_Identity=29.0322580645161, Blast_Score=186, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHAB_LISW6 (A0AMA3)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_850914.1
- ProteinModelPortal:   A0AMA3
- SMR:   A0AMA3
- STRING:   A0AMA3
- GeneID:   4466025
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe2717
- NMPDR:   fig|386043.6.peg.2643
- eggNOG:   COG2918
- HOGENOM:   HBG296652
- OMA:   PKSTNYG
- PhylomeDB:   A0AMA3
- ProtClustDB:   PRK02471
- BioCyc:   LWEL386043:LWE2717-MONOMER
- HAMAP:   MF_00782
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335
- InterPro:   IPR020561
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR01435

Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase

EC number: =6.3.2.2; =6.3.2.3

Molecular weight: Translated: 88616; Mature: 88616

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLDMTILDSLKENKALRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYI
CEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCEECCCCCCCEE
KTDFSESQIEMITPVTDSIDDVYNWLENLHNIVSLRSKNELLWPSSNPPILPAEKDIPIA
EECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCC
EYKTPDSPDRKYREHLAQGYGKKIQLLSGIHYNFSFPEALIDGLYDEISLPNESKRDFKN
CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
RLYLKVAKYFMKNRWLLIYLTGASPVYLADFTKTKQEEKLRDGSSALHDGISLRNSNAGY
HHHHHHHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHHHHCCHHHHHCCCEECCCCCCC
KNKESLYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAKHGVEY
CCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEE
LEIRSIDLNPLEPNGISKEALHFIHLFLIKGLLSEDRELCENNQQLADENENNIALNGLS
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEECCC
KPAIKNCDNEEMALADAGLLELDKMNDFIQSLRPEDTYFQAIIEKQKERLLHPEKTIAAQ
CCHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
VKEQSATAGFIEFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
HHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEHH
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAENNIRVPFGDSFSDQ
HHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCEECCCCCCCCCH
ALALEAFSLFKDKQIVVKPKSTNYGWGISIFKNKFTTEDYQEALNIAFSYDSSVIIEEFI
HHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHEEEECCCCCHHHHCC
PGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEKNMDPLRGTDHLKPLEKIRTG
CCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC
PEETLMLSMQKLSWDSIPKANETIYLRENSNVSTGGDSIDYTAEMDDYFKEIAIRATQVL
CCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEQKKIGDKILDFLFE
HHHHCCEEEEECHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKLDMTILDSLKENKALRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYI
CEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCEECCCCCCCEE
KTDFSESQIEMITPVTDSIDDVYNWLENLHNIVSLRSKNELLWPSSNPPILPAEKDIPIA
EECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCC
EYKTPDSPDRKYREHLAQGYGKKIQLLSGIHYNFSFPEALIDGLYDEISLPNESKRDFKN
CCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
RLYLKVAKYFMKNRWLLIYLTGASPVYLADFTKTKQEEKLRDGSSALHDGISLRNSNAGY
HHHHHHHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHHHHCCHHHHHCCCEECCCCCCC
KNKESLYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAKHGVEY
CCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEE
LEIRSIDLNPLEPNGISKEALHFIHLFLIKGLLSEDRELCENNQQLADENENNIALNGLS
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEECCC
KPAIKNCDNEEMALADAGLLELDKMNDFIQSLRPEDTYFQAIIEKQKERLLHPEKTIAAQ
CCHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
VKEQSATAGFIEFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLD
HHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEHH
RAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAENNIRVPFGDSFSDQ
HHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCEECCCCCCCCCH
ALALEAFSLFKDKQIVVKPKSTNYGWGISIFKNKFTTEDYQEALNIAFSYDSSVIIEEFI
HHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHEEEECCCCCHHHHCC
PGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEKNMDPLRGTDHLKPLEKIRTG
CCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC
PEETLMLSMQKLSWDSIPKANETIYLRENSNVSTGGDSIDYTAEMDDYFKEIAIRATQVL
CCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
DAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEQKKIGDKILDFLFE
HHHHCCEEEEECHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA