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The map label for this gene is pit [H]

Identifier: 116873681

GI number: 116873681

Start: 2303964

End: 2304962

Strand: Direct

Name: pit [H]

Synonym: lwe2265

Alternate gene names: 116873681

Gene position: 2303964-2304962 (Clockwise)

Preceding gene: 116873680

Following gene: 116873682

Centisome position: 81.87

GC content: 39.94

Gene sequence:

>999_bases
ATGGAAGGAATGTTTCTTATAACCGTCGTCATCGTTCTTGCTGCGCTTGCATTTGACCTTATTAACGGGTTTCATGATAC
AGCCAACGCAATTGCGACTAGTGTTTCTACAAAAGCTTTAAAACCACGACATGCAATTATCCTTGCAGCAGTAATGAACT
TTGTGGGTGCAATTTCATTCACAGGGGTGGCTAAAACGATTACTAAAGACATTGTGAACCCATTTGCTTTAGATCATGGG
GAACTTGTTATTTTAGCAGCATTACTTTCGGCTATAGCTTGGAATTTAATAACTTGGTATTTTGGAATTCCTAGTAGTTC
CTCTCATGCCTTAATTGGTTCCATCGCAGGTGCAGCAATTGCATCAGCAGGATTTGCATCAATTGAATACAGTGGCTTTA
CTAAAATCATCATCGGTTTATTAGTTTCTCCTGTACTCGCTTTCGTAGTTGGTTACACGATATATTCGCTCTTTAAGATT
TTCTTGAAGAACTTAAACTTGGCAACGACCAATCGGCGTTTCCGGATGATTCAAGTCGGTACTGCTGCGCTACAATCTTA
CACACATGGTACAAATGATGCACAAAAATCAATGGGGATCATCACAATGGCTTTAATTGCTAGTGGATTCCAAACAACAG
ACGATGTGCAATTATGGGTTCAAGTATCCTGTGCAATCGCTATGGCGGTCGGTACAAGTATTGGTGGTTGGAAAATCATC
AAAACAGTTGGTGGGAAAATCATGAAAATCAAGCCTGTGAATGGTGTAGCGGCTGACTTAAGTTCCGTTATCATTATTTT
CGGTGCCACTTTCATTCATTTACCAGTTAGTACAACGCACGTAATCAGCTCTTCTATCCTTGGTGTTGGAACAGCTCACC
GTGTTAAAGGAGTAAAATGGGATACTGCTCAACGCATGATTATTACATGGGTTATCACACTTCCTATTTCTGCAACGATT
GCTGCTCTTATCTTCTATGTACTAAGATTCTTTCTATAA

Upstream 100 bases:

>100_bases
GAAGTTTATGAAAAATTAGAAGACATTGCTGATAGTTGTCAAAGCGTTGCAAATACGCTTGAGTCAATTGTCATGAAAAA
TGCGTAAGGGGCCTAGATAG

Downstream 100 bases:

>100_bases
TTAATTTCCAGAGCCTTGCTTATGCAAGGCTCTTTTTATACATTTTTTTGAATAAAAACAAGGATATTAAGTATAAAACA
AGTTTATTTTAAAAACTTAT

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MEGMFLITVVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISFTGVAKTITKDIVNPFALDHG
ELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAIASAGFASIEYSGFTKIIIGLLVSPVLAFVVGYTIYSLFKI
FLKNLNLATTNRRFRMIQVGTAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAVGTSIGGWKII
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKWDTAQRMIITWVITLPISATI
AALIFYVLRFFL

Sequences:

>Translated_332_residues
MEGMFLITVVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISFTGVAKTITKDIVNPFALDHG
ELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAIASAGFASIEYSGFTKIIIGLLVSPVLAFVVGYTIYSLFKI
FLKNLNLATTNRRFRMIQVGTAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAVGTSIGGWKII
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKWDTAQRMIITWVITLPISATI
AALIFYVLRFFL
>Mature_332_residues
MEGMFLITVVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISFTGVAKTITKDIVNPFALDHG
ELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAIASAGFASIEYSGFTKIIIGLLVSPVLAFVVGYTIYSLFKI
FLKNLNLATTNRRFRMIQVGTAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAVGTSIGGWKII
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKWDTAQRMIITWVITLPISATI
AALIFYVLRFFL

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=160, Percent_Identity=29.375, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI5803173, Length=160, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789360, Length=241, Percent_Identity=30.7053941908714, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789907, Length=225, Percent_Identity=32.4444444444444, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71981576, Length=164, Percent_Identity=34.1463414634146, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17539280, Length=164, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=163, Percent_Identity=25.1533742331288, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32566716, Length=166, Percent_Identity=29.5180722891566, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=155, Percent_Identity=29.6774193548387, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35261; Mature: 35261

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGMFLITVVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVNPFALDHGELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ASAGFASIEYSGFTKIIIGLLVSPVLAFVVGYTIYSLFKIFLKNLNLATTNRRFRMIQVG
HHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEHH
TAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAVGTSIGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKW
HHHCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCHHHHCCCCC
DTAQRMIITWVITLPISATIAALIFYVLRFFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEGMFLITVVIVLAALAFDLINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAISF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVNPFALDHGELVILAALLSAIAWNLITWYFGIPSSSSHALIGSIAGAAI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ASAGFASIEYSGFTKIIIGLLVSPVLAFVVGYTIYSLFKIFLKNLNLATTNRRFRMIQVG
HHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEHH
TAALQSYTHGTNDAQKSMGIITMALIASGFQTTDDVQLWVQVSCAIAMAVGTSIGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
KTVGGKIMKIKPVNGVAADLSSVIIIFGATFIHLPVSTTHVISSSILGVGTAHRVKGVKW
HHHCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCHHHHCCCCC
DTAQRMIITWVITLPISATIAALIFYVLRFFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]