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The map label for this gene is adaA [H]

Identifier: 116873676

GI number: 116873676

Start: 2299778

End: 2300332

Strand: Direct

Name: adaA [H]

Synonym: lwe2260

Alternate gene names: 116873676

Gene position: 2299778-2300332 (Clockwise)

Preceding gene: 116873671

Following gene: 116873677

Centisome position: 81.72

GC content: 35.68

Gene sequence:

>555_bases
TTGTCAGATTATTACTTAACAAAAAAAAGATGGCACGCAATTTCTACAAATGATAAAGCTGCTGATGGCGAATTTTTCTA
TGGTGTAAAGTCTACGAAGATCTTCTGCTATCCGTCTTGTAAGTCCAGGCTGCCTAAAAAAGAAAATATTGTTATTTTTC
AAGATACTGATGATGCTATTTCGCATGGTTATCGCGCTTGCAAACGATGCAGGTCTGGTGGAAACGCACTTCCGGATACC
GAATGGGTAGAAAACATTGCGACTTATATTGACGAAAACTTTGCAAAACCATTAACTTTACAAATTATTGCCGAAGATTG
TCATGGCAGCCCTTATCATCTACATCGGACTTTTAAAAGAATTACCAAAATTACACCCATTACTTATCTAGATAACATTC
GAATAGACTATGCAAAAAAACAACTCTTAAACACCCTAGAATCCATTGAAGTTATTGGAAAAAAAGCAGGTTTTCCAAAT
CCGTCTCACTTTTCCACTACTTTTAAAAGACATACTGGATTATCACCAAATCGCTTTCGAAAAGGACAACAATAA

Upstream 100 bases:

>100_bases
ATTTCCAATTCACTTAGGTCATAAAAGCAAGATTAGAAAAGTTTTTCTCGCAATTTAGGTTAATAACGTTTATGATTAAT
AAGAAGTTGAGGTGATAACA

Downstream 100 bases:

>100_bases
AATAAATTAGAACGGATGAAACGAATATGACTCATTTAACATTACCTATTTTAGAAGACTGGGAAGGACTTACATTAACT
ACTATTTTGCAAGAAAAATT

Product: Ada regulatory protein, putative

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSDYYLTKKRWHAISTNDKAADGEFFYGVKSTKIFCYPSCKSRLPKKENIVIFQDTDDAISHGYRACKRCRSGGNALPDT
EWVENIATYIDENFAKPLTLQIIAEDCHGSPYHLHRTFKRITKITPITYLDNIRIDYAKKQLLNTLESIEVIGKKAGFPN
PSHFSTTFKRHTGLSPNRFRKGQQ

Sequences:

>Translated_184_residues
MSDYYLTKKRWHAISTNDKAADGEFFYGVKSTKIFCYPSCKSRLPKKENIVIFQDTDDAISHGYRACKRCRSGGNALPDT
EWVENIATYIDENFAKPLTLQIIAEDCHGSPYHLHRTFKRITKITPITYLDNIRIDYAKKQLLNTLESIEVIGKKAGFPN
PSHFSTTFKRHTGLSPNRFRKGQQ
>Mature_183_residues
SDYYLTKKRWHAISTNDKAADGEFFYGVKSTKIFCYPSCKSRLPKKENIVIFQDTDDAISHGYRACKRCRSGGNALPDTE
WVENIATYIDENFAKPLTLQIIAEDCHGSPYHLHRTFKRITKITPITYLDNIRIDYAKKQLLNTLESIEVIGKKAGFPNP
SHFSTTFKRHTGLSPNRFRKGQQ

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=176, Percent_Identity=31.8181818181818, Blast_Score=106, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 21169; Mature: 21038

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYYLTKKRWHAISTNDKAADGEFFYGVKSTKIFCYPSCKSRLPKKENIVIFQDTDDAI
CCCCCCCHHHEEEECCCCCCCCCCEEEEECCCEEEECCCHHHHCCCCCCEEEEECCCHHH
SHGYRACKRCRSGGNALPDTEWVENIATYIDENFAKPLTLQIIAEDCHGSPYHLHRTFKR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
ITKITPITYLDNIRIDYAKKQLLNTLESIEVIGKKAGFPNPSHFSTTFKRHTGLSPNRFR
HHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHC
KGQQ
CCCC
>Mature Secondary Structure 
SDYYLTKKRWHAISTNDKAADGEFFYGVKSTKIFCYPSCKSRLPKKENIVIFQDTDDAI
CCCCCCHHHEEEECCCCCCCCCCEEEEECCCEEEECCCHHHHCCCCCCEEEEECCCHHH
SHGYRACKRCRSGGNALPDTEWVENIATYIDENFAKPLTLQIIAEDCHGSPYHLHRTFKR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
ITKITPITYLDNIRIDYAKKQLLNTLESIEVIGKKAGFPNPSHFSTTFKRHTGLSPNRFR
HHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHC
KGQQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]