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The map label for this gene is trpS [H]

Identifier: 116873631

GI number: 116873631

Start: 2253270

End: 2254265

Strand: Direct

Name: trpS [H]

Synonym: lwe2215

Alternate gene names: 116873631

Gene position: 2253270-2254265 (Clockwise)

Preceding gene: 116873630

Following gene: 116873637

Centisome position: 80.07

GC content: 35.44

Gene sequence:

>996_bases
ATGAAAAAAGTAATATTTTCCGGCATTCAACCTAGTGGACAACTAACTTTAGGGAATTATATTGGTGCTTTAAAGCAATT
TGGGCAATTTCAAGATGAATTTGAGTGTTTTTATTGTATTGTGGATGAACATGCTATAACAGTGCCGCAAGATCGCTTAA
AGCTGCGTGAGCAAACTAGAAGTCTTGCCGCTCTTTATTTAGCAGTTGGACTAAATCCAGAAAAAGCGACATTATTTATT
CAATCAGAAGTTGCAGCACACGCGCAAGCTGCTTGGATTTTACAATGTAACGTCTATATTGGCGAACTAGAACGTATGAC
ACAGTTTAAAGATAAATCAGATGGTAAGGCTGGCGTTAGTGCTGGACTTTTGACTTATCCTCCACTAATGGCTGCCGATA
TTTTACTTTATCAAACGAATTTAGTACCTGTAGGCGAAGATCAAAAGCAACATATTGAACTGACACGTGATTTAGCAGAT
AGATTCAATAAAAAACATGCTGATATATTTACAATGCCAGAAGTTTTCATTCCTAAACAAGGGGCTAGAGTCATGTCCCT
TCAAGATCCAACTAAAAAAATGAGTAAATCAGATGCTAATTTAAAAAATGCCATATTCTTACTTGACCCACCCGCAACTA
TTAGAAAAAAAATTAAAAGCGCAGTGACAGATTCCAGTGGCATTATTGAATATAATAAAGAAGAAAAACCTGGTGTTTCC
AACTTACTCACAATATATTCTGTTGTAACTGGAGAAACAATCGCTAGCATTGAAGAAAAATATGTTGGTAAAGGTTATGG
TGATTTTAAAACAGATCTAGCAGAATTAGTGGTTGCAGAACTTGAACCAATTCAAGAAAAATATTACGCTTACTTAGAAT
CTGAAGAATTGGATAATATTTTAGATGCTGGAGCAGAAAAAGCAGCAAAAGTAGCTAATAAAACACTGAAGAAAATGGAA
AACGGCATAGGACTAGGCCGCAAACGCAGAAAATAA

Upstream 100 bases:

>100_bases
AGGGTGGCACCGCGGATAACCGTTCGTCCCTAACATTTTATATGTTGGGATGGACGGTTTTTTATTTTCGCAAATCAGTG
AAAAAATTGGAGGAATAAAC

Downstream 100 bases:

>100_bases
CAAAAAAGGGATTACCTGGTTATCCAAGTAATCCCTTTTTAAAATTTTAATTAACCTACTTTGATTTCCACATCAATGTT
ACCACGTGTTGCTTTGGAAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEFECFYCIVDEHAITVPQDRLKLREQTRSLAALYLAVGLNPEKATLFI
QSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVSAGLLTYPPLMAADILLYQTNLVPVGEDQKQHIELTRDLAD
RFNKKHADIFTMPEVFIPKQGARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
NLLTIYSVVTGETIASIEEKYVGKGYGDFKTDLAELVVAELEPIQEKYYAYLESEELDNILDAGAEKAAKVANKTLKKME
NGIGLGRKRRK

Sequences:

>Translated_331_residues
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEFECFYCIVDEHAITVPQDRLKLREQTRSLAALYLAVGLNPEKATLFI
QSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVSAGLLTYPPLMAADILLYQTNLVPVGEDQKQHIELTRDLAD
RFNKKHADIFTMPEVFIPKQGARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
NLLTIYSVVTGETIASIEEKYVGKGYGDFKTDLAELVVAELEPIQEKYYAYLESEELDNILDAGAEKAAKVANKTLKKME
NGIGLGRKRRK
>Mature_331_residues
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEFECFYCIVDEHAITVPQDRLKLREQTRSLAALYLAVGLNPEKATLFI
QSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVSAGLLTYPPLMAADILLYQTNLVPVGEDQKQHIELTRDLAD
RFNKKHADIFTMPEVFIPKQGARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
NLLTIYSVVTGETIASIEEKYVGKGYGDFKTDLAELVVAELEPIQEKYYAYLESEELDNILDAGAEKAAKVANKTLKKME
NGIGLGRKRRK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=327, Percent_Identity=40.6727828746177, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI41352700, Length=175, Percent_Identity=52.5714285714286, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=56.0975609756098, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71982800, Length=330, Percent_Identity=36.969696969697, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71982793, Length=330, Percent_Identity=36.969696969697, Blast_Score=210, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6320474, Length=348, Percent_Identity=39.9425287356322, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24666151, Length=325, Percent_Identity=43.0769230769231, Blast_Score=258, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36831; Mature: 36831

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEFECFYCIVDEHAITVPQDRLKLREQTR
CCCCEECCCCCCCCEEHHHHHHHHHHHHCCHHCCEEEEEEEECCEEECCHHHHHHHHHHH
SLAALYLAVGLNPEKATLFIQSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVS
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCCCCCCCCC
AGLLTYPPLMAADILLYQTNLVPVGEDQKQHIELTRDLADRFNKKHADIFTMPEVFIPKQ
CCCHHCCHHHHHHHHHEECCCEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCC
GARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
CCEEEECCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCCHH
NLLTIYSVVTGETIASIEEKYVGKGYGDFKTDLAELVVAELEPIQEKYYAYLESEELDNI
HHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAGAEKAAKVANKTLKKMENGIGLGRKRRK
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEFECFYCIVDEHAITVPQDRLKLREQTR
CCCCEECCCCCCCCEEHHHHHHHHHHHHCCHHCCEEEEEEEECCEEECCHHHHHHHHHHH
SLAALYLAVGLNPEKATLFIQSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVS
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCCCCCCCCC
AGLLTYPPLMAADILLYQTNLVPVGEDQKQHIELTRDLADRFNKKHADIFTMPEVFIPKQ
CCCHHCCHHHHHHHHHEECCCEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCC
GARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
CCEEEECCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCCHH
NLLTIYSVVTGETIASIEEKYVGKGYGDFKTDLAELVVAELEPIQEKYYAYLESEELDNI
HHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAGAEKAAKVANKTLKKMENGIGLGRKRRK
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]