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The map label for this gene is pdxT

Identifier: 116873539

GI number: 116873539

Start: 2155470

End: 2156036

Strand: Direct

Name: pdxT

Synonym: lwe2123

Alternate gene names: 116873539

Gene position: 2155470-2156036 (Clockwise)

Preceding gene: 116873538

Following gene: 116873541

Centisome position: 76.59

GC content: 40.56

Gene sequence:

>567_bases
ATGAAAAAAATTGGTGTTCTTGCACTTCAAGGTGCTGTCGACGAGCATATCCAAATGATTAAATCTGCCGGTGCTCTCCC
TTCAAAAGTAAAGCACCCGAGTGATTTAAACGAGCTTGATGGACTTGTTTTACCAGGTGGAGAAAGTACAACTATGCGCA
AAATCATGAAACGCTATGATTTAATAGAACCTGTTAGAGCATTTGCTAAAGAAGGAAAAGCTATTTTTGGTACTTGTGCC
GGACTTGTCCTTTTGTCCAAAGAAATCGAAGGCGGCGAAGAAAGCCTAGGATTAATTGACACAACCGCTGTTCGTAATGG
TTTCGGTCGGCAGAAAGAAAGTTTTGAAGCTGAGTTAAACGTCGAAATATTTAGTGATGCTCCTTTTGAAGCTGTATTTA
TCCGTGCCCCCTACTTAATCGAACCAAGTCCCGAAGTATCTGTGTTAGCAACCTTTGAAGATAAAATTGTAGCAGCTAAA
GAGGCCAATATTCTCGTCACCGCGTTCCATCCAGAACTAACAAACGATAACCGTTTCATGCGTTTCTTTATCGAAAAAAT
GGTATAA

Upstream 100 bases:

>100_bases
TTAATCGGAAAACTTTCCAAAGAGCTAGGTTCCCCGATGAAAGGAATCGAAATGTCTCGCCTAAACCCAGAGGACAGAAT
GCAAGATCGGAGTGTTTAAT

Downstream 100 bases:

>100_bases
AAAAAAGCAGTAGACACATGCGTCTACTGCTTTTACCTATTATTTATTCACTGCTTGCGCTGCTGTAATTAATGTTAAGT
TATAAACATCATCTGTATTA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKKIGVLALQGAVDEHIQMIKSAGALPSKVKHPSDLNELDGLVLPGGESTTMRKIMKRYDLIEPVRAFAKEGKAIFGTCA
GLVLLSKEIEGGEESLGLIDTTAVRNGFGRQKESFEAELNVEIFSDAPFEAVFIRAPYLIEPSPEVSVLATFEDKIVAAK
EANILVTAFHPELTNDNRFMRFFIEKMV

Sequences:

>Translated_188_residues
MKKIGVLALQGAVDEHIQMIKSAGALPSKVKHPSDLNELDGLVLPGGESTTMRKIMKRYDLIEPVRAFAKEGKAIFGTCA
GLVLLSKEIEGGEESLGLIDTTAVRNGFGRQKESFEAELNVEIFSDAPFEAVFIRAPYLIEPSPEVSVLATFEDKIVAAK
EANILVTAFHPELTNDNRFMRFFIEKMV
>Mature_188_residues
MKKIGVLALQGAVDEHIQMIKSAGALPSKVKHPSDLNELDGLVLPGGESTTMRKIMKRYDLIEPVRAFAKEGKAIFGTCA
GLVLLSKEIEGGEESLGLIDTTAVRNGFGRQKESFEAELNVEIFSDAPFEAVFIRAPYLIEPSPEVSVLATFEDKIVAAK
EANILVTAFHPELTNDNRFMRFFIEKMV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=210, Percent_Identity=39.5238095238095, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323995, Length=212, Percent_Identity=37.7358490566038, Blast_Score=108, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=37.7358490566038, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_LISW6 (A0AKK9)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_850320.1
- ProteinModelPortal:   A0AKK9
- SMR:   A0AKK9
- STRING:   A0AKK9
- GeneID:   4463996
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe2123
- NMPDR:   fig|386043.6.peg.2064
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   A0AKK9
- ProtClustDB:   PRK13525
- BioCyc:   LWEL386043:LWE2123-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20669; Mature: 20669

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 79-79 ACT_SITE 170-170 ACT_SITE 172-172 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGVLALQGAVDEHIQMIKSAGALPSKVKHPSDLNELDGLVLPGGESTTMRKIMKRYD
CCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHH
LIEPVRAFAKEGKAIFGTCAGLVLLSKEIEGGEESLGLIDTTAVRNGFGRQKESFEAELN
HHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCCCCCCCEEEE
VEIFSDAPFEAVFIRAPYLIEPSPEVSVLATFEDKIVAAKEANILVTAFHPELTNDNRFM
EEEECCCCCEEEEEECCEEECCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCHHH
RFFIEKMV
HHHHHHHC
>Mature Secondary Structure
MKKIGVLALQGAVDEHIQMIKSAGALPSKVKHPSDLNELDGLVLPGGESTTMRKIMKRYD
CCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHH
LIEPVRAFAKEGKAIFGTCAGLVLLSKEIEGGEESLGLIDTTAVRNGFGRQKESFEAELN
HHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCCCCCCCEEEE
VEIFSDAPFEAVFIRAPYLIEPSPEVSVLATFEDKIVAAKEANILVTAFHPELTNDNRFM
EEEECCCCCEEEEEECCEEECCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCHHH
RFFIEKMV
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA