Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 116873500

GI number: 116873500

Start: 2117488

End: 2117880

Strand: Direct

Name: mscL

Synonym: lwe2084

Alternate gene names: 116873500

Gene position: 2117488-2117880 (Clockwise)

Preceding gene: 116873499

Following gene: 116873507

Centisome position: 75.24

GC content: 33.59

Gene sequence:

>393_bases
ATGAAAAAAATGTTAGTAGAATTTAGAGATTTTGCGTTAAAAGGGAATGTACTAGACCTTGCTGTAGCGGTTGTTATCGG
GGCAGCATTTGGAAAAATTGTTTCTTCTTTAGTAGATAACATCATTATGCCAGTTGTTGGTGTACTTCTTGGCGGACTTG
ATTTCACAAAATTAAGTGTGACAGTCGGTAAATCTGTTATTCAGTACGGTGCTTTCATCCAATCTATCGTTGACTTTATC
ATCATCGCTTTTGCTATTTTTATATTCGTCAAAATACTTACTAGTTTTATGAAGAAAAAAGAACAACCTGTGGAAGAAAC
ACCAGTGCCACCAACAGAAGAATACTTAAAAGAAATTCGCGATTTATTGAAAGAACAACAAAAAGAAATTTAA

Upstream 100 bases:

>100_bases
GCGCCAACTCTATACATAGGCAGTTTATTATTAAAATAAGTTTAGTTACTTTCACATCGCCTTTAGATGTTTTATACTGG
GGAGTGGGAGGTGAATTTTT

Downstream 100 bases:

>100_bases
AAGGAAAGACAGAAACTCTAGTCAGCTATTTTTGACTAAAAGTTCCTGTCTTTTTTATTGTTGTTGTTTCTTTAATTGGA
GTGCTAAGAACATTTCAAAC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPVVGVLLGGLDFTKLSVTVGKSVIQYGAFIQSIVDFI
IIAFAIFIFVKILTSFMKKKEQPVEETPVPPTEEYLKEIRDLLKEQQKEI

Sequences:

>Translated_130_residues
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPVVGVLLGGLDFTKLSVTVGKSVIQYGAFIQSIVDFI
IIAFAIFIFVKILTSFMKKKEQPVEETPVPPTEEYLKEIRDLLKEQQKEI
>Mature_130_residues
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPVVGVLLGGLDFTKLSVTVGKSVIQYGAFIQSIVDFI
IIAFAIFIFVKILTSFMKKKEQPVEETPVPPTEEYLKEIRDLLKEQQKEI

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=131, Percent_Identity=54.1984732824427, Blast_Score=138, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_LISW6 (A0AKH0)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_850281.1
- ProteinModelPortal:   A0AKH0
- SMR:   A0AKH0
- STRING:   A0AKH0
- GeneID:   4465355
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe2084
- NMPDR:   fig|386043.6.peg.2027
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   A0AKH0
- ProtClustDB:   PRK13953
- BioCyc:   LWEL386043:LWE2084-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14434; Mature: 14434

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b30dbd4)-; HASH(0x1c98469c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPVVGVLLGGLDFTKLSV
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TVGKSVIQYGAFIQSIVDFIIIAFAIFIFVKILTSFMKKKEQPVEETPVPPTEEYLKEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
DLLKEQQKEI
HHHHHHHHCH
>Mature Secondary Structure
MKKMLVEFRDFALKGNVLDLAVAVVIGAAFGKIVSSLVDNIIMPVVGVLLGGLDFTKLSV
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TVGKSVIQYGAFIQSIVDFIIIAFAIFIFVKILTSFMKKKEQPVEETPVPPTEEYLKEIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
DLLKEQQKEI
HHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA