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The map label for this gene is exoA [H]

Identifier: 116873216

GI number: 116873216

Start: 1832551

End: 1833306

Strand: Direct

Name: exoA [H]

Synonym: lwe1800

Alternate gene names: 116873216

Gene position: 1832551-1833306 (Clockwise)

Preceding gene: 116873213

Following gene: 116873218

Centisome position: 65.12

GC content: 38.62

Gene sequence:

>756_bases
ATGAAATTAATTTCTTGGAATGTAAATGGACTTCGAGCAGCTGTAAAAAAAGGCTTTTTGGAGTATTTTGAAGAAGTGGA
TGCAGATATTTTTTGTTTGCAGGAGACAAAATTACAAGAAGGTCAAATCGAACTGGATTTAAAAGCGTATAAGGATTACT
GGAATTATGCAGTGAAAAAAGGTTATTCTGGTACCGCGATTTTTACGAAAGTGGAACCATTGTCTGTGCAATATGGCTTA
GGGATACCGGAGCATGATACAGAAGGTCGCGTTATCACCCTTGAGTTTGAAGCGTTTTTTATGGTGACAGTTTATACCCC
AAATTCGCAAGCTGAATTAAAACGTTTGGATTATCGTATGACTTTTGAAGATGCGATTTTGGAATATATAAAGAATTTGG
ATAAAACAAAACCGGTTGTGCTTTGTGGTGACTTGAATGTTGCGCACGAGGAAATTGATTTAAAAAACCCAAAGACAAAT
CGCAAAAATGCAGGATTCTCGGACGAGGAACGCGCGAAGTTTTCTGCGTTCTTAGATGCTGGATTTATTGATAGTTTCCG
TTACTTTTATCCAGATTTGACTGATGCTTATTCGTGGTGGTCTTACCGAATGAACGCTCGTGCTAGGAATACTGGCTGGC
GGATTGACTATTTTGTTGTTTCCAAGCGACTGAAAGACAAATTAGTGGATGCCAAAATCCATTCGGAAGTACTTGGTTCA
GATCATTGCCCGGTGGAACTGGAACTTAATTTATAG

Upstream 100 bases:

>100_bases
AAGCGACGTATTTTTCCATCTAGTCCCCCCCAATCGTTTAATTATATTATAGCATGTCACATACGTTATAATAGAGCTAT
TAGTGATGGGAGGTCTTTAG

Downstream 100 bases:

>100_bases
AAAACGAGTTTGCTTCGTGCAAACTCGTTTTTTTATGTCCTCACTAATTCAGTAATAGATTGAATGGTTTCTTTTAAATA
ATTGTATTCTTTTTTCTTCT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLKAYKDYWNYAVKKGYSGTAIFTKVEPLSVQYGL
GIPEHDTEGRVITLEFEAFFMVTVYTPNSQAELKRLDYRMTFEDAILEYIKNLDKTKPVVLCGDLNVAHEEIDLKNPKTN
RKNAGFSDEERAKFSAFLDAGFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSKRLKDKLVDAKIHSEVLGS
DHCPVELELNL

Sequences:

>Translated_251_residues
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLKAYKDYWNYAVKKGYSGTAIFTKVEPLSVQYGL
GIPEHDTEGRVITLEFEAFFMVTVYTPNSQAELKRLDYRMTFEDAILEYIKNLDKTKPVVLCGDLNVAHEEIDLKNPKTN
RKNAGFSDEERAKFSAFLDAGFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSKRLKDKLVDAKIHSEVLGS
DHCPVELELNL
>Mature_251_residues
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLKAYKDYWNYAVKKGYSGTAIFTKVEPLSVQYGL
GIPEHDTEGRVITLEFEAFFMVTVYTPNSQAELKRLDYRMTFEDAILEYIKNLDKTKPVVLCGDLNVAHEEIDLKNPKTN
RKNAGFSDEERAKFSAFLDAGFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSKRLKDKLVDAKIHSEVLGS
DHCPVELELNL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=257, Percent_Identity=50.1945525291829, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI18375503, Length=257, Percent_Identity=50.1945525291829, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI18375501, Length=257, Percent_Identity=50.1945525291829, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI18375507, Length=316, Percent_Identity=27.8481012658228, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=28.5171102661597, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=45.136186770428, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=39.3103448275862, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=49.2125984251969, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=49.2125984251969, Blast_Score=265, Evalue=2e-71,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29129; Mature: 29129

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLKAYKDYWNYAVKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHC
GYSGTAIFTKVEPLSVQYGLGIPEHDTEGRVITLEFEAFFMVTVYTPNSQAELKRLDYRM
CCCCCEEEEEECCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHEEE
TFEDAILEYIKNLDKTKPVVLCGDLNVAHEEIDLKNPKTNRKNAGFSDEERAKFSAFLDA
CHHHHHHHHHHCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSKRLKDKLVDAKIHSEVLGS
CHHHHHHHHCCCHHHHHHHHHEEECCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
DHCPVELELNL
CCCCEEEEECC
>Mature Secondary Structure
MKLISWNVNGLRAAVKKGFLEYFEEVDADIFCLQETKLQEGQIELDLKAYKDYWNYAVKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHC
GYSGTAIFTKVEPLSVQYGLGIPEHDTEGRVITLEFEAFFMVTVYTPNSQAELKRLDYRM
CCCCCEEEEEECCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHEEE
TFEDAILEYIKNLDKTKPVVLCGDLNVAHEEIDLKNPKTNRKNAGFSDEERAKFSAFLDA
CHHHHHHHHHHCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GFIDSFRYFYPDLTDAYSWWSYRMNARARNTGWRIDYFVVSKRLKDKLVDAKIHSEVLGS
CHHHHHHHHCCCHHHHHHHHHEEECCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
DHCPVELELNL
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]