Click here to switch to the map view.

The map label for this gene is yrvJ [H]

Identifier: 116872950

GI number: 116872950

Start: 1531768

End: 1533051

Strand: Direct

Name: yrvJ [H]

Synonym: lwe1534

Alternate gene names: 116872950

Gene position: 1531768-1533051 (Clockwise)

Preceding gene: 116872947

Following gene: 116873007

Centisome position: 54.43

GC content: 37.93

Gene sequence:

>1284_bases
ATGAAGAATAAATTCATTTTTATCACCGTTGTCTCCATTTTACTGATTGCAGCAGGTATTGTTACAACCATTGCAATGGC
GAACGCAAATTCAGTCATTGTAAAGGCAGAAGTATTAAATGTCCGCAGCGGTCCTGGTTTGGCTTATGATGTTACAAGTC
AAGCTAGAAAAAATGAAGTACTCCGGGTAGTCGGTGAAGAAAACGAATGGTACAAAGTCCAATTAGATAATGGTAATACT
GGTTGGGTTGCAAGTTGGTTAGTTGAAAATACAGACGTCAGTGCAGCAAGTAACAGTGTAGCTATTGTTTCATCTGATGG
TGGTCTAAATGTACGCGAAAAACCAAGTACATCTAGTGCCTCGCTTGGATTACTAAATAATGGTGATCAAGTAACTGTAA
CAAGTCAACAAAATGGCTGGGCACAAATCCAGTATAAAGGGAAAAGCGCGTGGGTAAGTTCCGATTTCCTAAACATTCGC
GAATCTGTAACAAAAGTTGACGAAAGTGAACTACAAACAGTAACAATTCGTGAAGACTCCACAAATATTCGAAATAAACC
AAGCCGAGATGGTGACGTTATTGAAAAAGCTAATTCCGGCCAAGGTTTTGCTATTCAAGGTGTCCAAGGTGATTGGTATA
AAATTCGTACAACTAGCGGACAAGAAGGTTATGTAGCCAATTGGGTTGTAGATGTATCCGATAAAGGTCAAACATCCTCC
CCTCGAAGCAAAACAACGAAATTATCTGAAGCGACTATTGTTATCGATCCCGGTCACGGAGGAAATGACCCTGGAGCAAA
AGGCGCAAATGGAACCATTGAAAAAGAAATGACTTTAAAAACTGCTAAGAAATTAAAATCAAAATTAGAATCTAGAGGCG
CCAAAGTAATTTTAACGAGAAATAGTGATAAATATGTTTCCTTAAAATCAAGAACGAATGTCGCAGCTGAAAATAATGCA
GATGTCTTTATCAGTATCCATTTTGATAGTTTAGAGGACAGTAATTCTGGAGTTAGTGGACAAACAACTTATTACAATAA
TAATAATGATAAATCGCTTGCCGAAAGCATTAATGAAAGTCTTGGCAGCGACCTCCCTACTACTAACAGAGGAGCTAGAG
TTGGCGATTATTATGTAGTAAGAGAAAACTCCCAACCGGCTGTATTGCTCGAACTTGGCTATCTAAGCTCTAAAAAAGAC
GAACGAAATATTAATTCAGCTTCTTACAGAAGTCAAATTGCAGATTCCGTTACAGATGGCTTATCGAATTATTTTTCTAA
TTAA

Upstream 100 bases:

>100_bases
TTTATAAAAACCTACTTTCTAGTTCCCTGAAAGCGCATTTTATGGTAACATTTTACTGTCAGATAAAAGTTTATGAGTAT
ATGGGTGGTGTCAGCTTTAA

Downstream 100 bases:

>100_bases
AAAAAGCAATCATTCAGACGATTTTTCCATCTGAATGATTGCTTTTTATTTGCGCATTTCTGCGGAGTCTAACATCATTG
TGATTGGTCCATGATTAATG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKNKFIFITVVSILLIAAGIVTTIAMANANSVIVKAEVLNVRSGPGLAYDVTSQARKNEVLRVVGEENEWYKVQLDNGNT
GWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSASLGLLNNGDQVTVTSQQNGWAQIQYKGKSAWVSSDFLNIR
ESVTKVDESELQTVTIREDSTNIRNKPSRDGDVIEKANSGQGFAIQGVQGDWYKIRTTSGQEGYVANWVVDVSDKGQTSS
PRSKTTKLSEATIVIDPGHGGNDPGAKGANGTIEKEMTLKTAKKLKSKLESRGAKVILTRNSDKYVSLKSRTNVAAENNA
DVFISIHFDSLEDSNSGVSGQTTYYNNNNDKSLAESINESLGSDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSKKD
ERNINSASYRSQIADSVTDGLSNYFSN

Sequences:

>Translated_427_residues
MKNKFIFITVVSILLIAAGIVTTIAMANANSVIVKAEVLNVRSGPGLAYDVTSQARKNEVLRVVGEENEWYKVQLDNGNT
GWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSASLGLLNNGDQVTVTSQQNGWAQIQYKGKSAWVSSDFLNIR
ESVTKVDESELQTVTIREDSTNIRNKPSRDGDVIEKANSGQGFAIQGVQGDWYKIRTTSGQEGYVANWVVDVSDKGQTSS
PRSKTTKLSEATIVIDPGHGGNDPGAKGANGTIEKEMTLKTAKKLKSKLESRGAKVILTRNSDKYVSLKSRTNVAAENNA
DVFISIHFDSLEDSNSGVSGQTTYYNNNNDKSLAESINESLGSDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSKKD
ERNINSASYRSQIADSVTDGLSNYFSN
>Mature_427_residues
MKNKFIFITVVSILLIAAGIVTTIAMANANSVIVKAEVLNVRSGPGLAYDVTSQARKNEVLRVVGEENEWYKVQLDNGNT
GWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSASLGLLNNGDQVTVTSQQNGWAQIQYKGKSAWVSSDFLNIR
ESVTKVDESELQTVTIREDSTNIRNKPSRDGDVIEKANSGQGFAIQGVQGDWYKIRTTSGQEGYVANWVVDVSDKGQTSS
PRSKTTKLSEATIVIDPGHGGNDPGAKGANGTIEKEMTLKTAKKLKSKLESRGAKVILTRNSDKYVSLKSRTNVAAENNA
DVFISIHFDSLEDSNSGVSGQTTYYNNNNDKSLAESINESLGSDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSKKD
ERNINSASYRSQIADSVTDGLSNYFSN

Specific function: Probably involved in cell-wall metabolism [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=28.0172413793103, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790611, Length=223, Percent_Identity=29.5964125560538, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082163, Length=221, Percent_Identity=26.6968325791855, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR017293
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46118; Mature: 46118

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKFIFITVVSILLIAAGIVTTIAMANANSVIVKAEVLNVRSGPGLAYDVTSQARKNEV
CCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEECCCHHHCCCE
LRVVGEENEWYKVQLDNGNTGWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSA
EEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
SLGLLNNGDQVTVTSQQNGWAQIQYKGKSAWVSSDFLNIRESVTKVDESELQTVTIREDS
EEEEECCCCEEEEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHCCCCCCEEEEEECCC
TNIRNKPSRDGDVIEKANSGQGFAIQGVQGDWYKIRTTSGQEGYVANWVVDVSDKGQTSS
CCCCCCCCCCCHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCC
PRSKTTKLSEATIVIDPGHGGNDPGAKGANGTIEKEMTLKTAKKLKSKLESRGAKVILTR
CCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
NSDKYVSLKSRTNVAAENNADVFISIHFDSLEDSNSGVSGQTTYYNNNNDKSLAESINES
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
LGSDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSKKDERNINSASYRSQIADSVTDG
HCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSNYFSN
HHHHHCC
>Mature Secondary Structure
MKNKFIFITVVSILLIAAGIVTTIAMANANSVIVKAEVLNVRSGPGLAYDVTSQARKNEV
CCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEECCCHHHCCCE
LRVVGEENEWYKVQLDNGNTGWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSA
EEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
SLGLLNNGDQVTVTSQQNGWAQIQYKGKSAWVSSDFLNIRESVTKVDESELQTVTIREDS
EEEEECCCCEEEEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHCCCCCCEEEEEECCC
TNIRNKPSRDGDVIEKANSGQGFAIQGVQGDWYKIRTTSGQEGYVANWVVDVSDKGQTSS
CCCCCCCCCCCHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCC
PRSKTTKLSEATIVIDPGHGGNDPGAKGANGTIEKEMTLKTAKKLKSKLESRGAKVILTR
CCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
NSDKYVSLKSRTNVAAENNADVFISIHFDSLEDSNSGVSGQTTYYNNNNDKSLAESINES
CCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
LGSDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSKKDERNINSASYRSQIADSVTDG
HCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSNYFSN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]