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The map label for this gene is recN [H]

Identifier: 116872799

GI number: 116872799

Start: 1369634

End: 1371325

Strand: Direct

Name: recN [H]

Synonym: lwe1383

Alternate gene names: 116872799

Gene position: 1369634-1371325 (Clockwise)

Preceding gene: 116872798

Following gene: 116872800

Centisome position: 48.67

GC content: 37.65

Gene sequence:

>1692_bases
TTGCTTCAAGAAATGACAATTAAAAACTTTGCTATCATCGAATCACTCTCTTTAACTTTTCAAGAAGGGATGACAGTATT
AACGGGGGAGACCGGCGCAGGTAAATCAATTATTATTGATGCGCTTGGTCTTCTTGTTGGCGGACGTGGTTCCACTGACT
TTATTCGTCACGGTGAGGAACGCCTAGAACTTCAAGGCCTTTTTGCGTTAGCAGAGGATAATTTTGCTTGCCGTAATGCT
CTTTTGGAAAATGGAATTGATGCTTCAGATAATATGGTCGTTTTTGAACGGAGCTTATTTCGTTCCGGTAAAAATAGTTG
CCGTATTAATGGTAAACTTGTTACTACTGTTCTGCTTCGTCAAATTGGTTCTAAATTAATTGATATCCATAGTCAACATG
AGCATCAAGAATTAATGAATGAGGAATTTCATTTGTCACTACTAGATCGTTTTGCTTCAGACAAAATTAAACCTGCATTG
ACGAAATACCAAACTAATTTCAAAGAATTTCAAACGATTGTAAGAGAGTGGCAAAACTGGACAAAAAACGAGCGAGAACT
TGCACAGCGACTAGATATGCTGCGTTTTCAACAGCAAGAAATCGAAAACGCTAATTTACAAGCAGGGGAAGAAGATCGTT
TATTAGAGCAAAAAAATATTTTAGCTAATTTTGAAAAGTTGAATGAAAACTTACAAGGTGCATACACGGCTATCCAAGGA
GAACCCGGCGGACTTGAATTCATTGGGGAAGCAATGCGCCAAATGGAGACAGCCGCGAGTATTCATACGGATTATAAAGC
GGTTAGTGAAGCGATTTCCTCCAGTTACTATATGTTAGAAGATAGCATGAGCCAAATTAGACAATCGCTTGATCAGCTTG
AATTCCAACCAGACGAACTCAATCAGATTGAATCGCGCTTGAATGACTTAAATCAATTGAAGCGGAAGTATGGCAAAACT
ATTGAAGATATCATCCAGTACGAGCAAGAAATCAGCAGAGAAATGGAAAAATTAACTAATAGTGAGTCACATGTGGGTCA
TTTAGAAACAAAAATGGCCACATTAAAAACCGAGCTTACAAAACAAGCAAGTGCGTTAACGGATATCCGTAAGAAAGCCG
CGGCTACATTAGAAAAACAAATTAAACAAGAATTAAATCAACTTTATATGGAAAAAGCTATTTTTAGTGTCCGATTTGAA
TCCAATAAAACAGAGTTGACAGAATCTGGACAAGATAATGTGGTTTTCTACATGTCAACCAATCCTGGAGAACCATTAAA
ACCTCTCGCAAAAATCGCTTCAGGCGGTGAACTTTCACGTATGATGTTGGCGCTAAAAACTATTTTTTCTAGACATCAAG
GAATTACATCCATCATTTTTGATGAAGTGGATACGGGTGTAAGTGGCCGTGTAGGGCAAGCTATTGCGGAAAAAATCTAT
GCCGTCTCAGTTGGCTCGCAAGTTCTTTGTATCAGTCATTTACCTCAAGTGGCTGCTATGGCAAACCATCATTACTACAT
CACAAAAAAAGTGCAAAATAAACGTACAACCACTTCAGTAACAATTCTAAAAGGCGATGAAAAAGTGGAAGAAATTAGCC
GAATGATTGCAGGAATTGAAGTGACTGATTTAACAAAACAACATGCGAAAGAAATGATTCAACAAGCAGAAAAAGTAAAA
CAAACTTATTAA

Upstream 100 bases:

>100_bases
GATGACACATGTTTAATCATTTGCCGTTCAGAAGAAAATGCAAAAACATTAACAGATCGTTTTATAGATATGCTTTAAAA
ATTGTTGGAGGTGAAGGATT

Downstream 100 bases:

>100_bases
AAAAACGAAATTGCTTGCATATCGAATAGTTTTTGCTTAAAATGAATAAGGTAGAAATTATTACCTGGTCATAAAAACAA
CTACTAATTCGAAATGCGGC

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSTDFIRHGEERLELQGLFALAEDNFACRNA
LLENGIDASDNMVVFERSLFRSGKNSCRINGKLVTTVLLRQIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPAL
TKYQTNFKEFQTIVREWQNWTKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYTAIQG
EPGGLEFIGEAMRQMETAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPDELNQIESRLNDLNQLKRKYGKT
IEDIIQYEQEISREMEKLTNSESHVGHLETKMATLKTELTKQASALTDIRKKAAATLEKQIKQELNQLYMEKAIFSVRFE
SNKTELTESGQDNVVFYMSTNPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTILKGDEKVEEISRMIAGIEVTDLTKQHAKEMIQQAEKVK
QTY

Sequences:

>Translated_563_residues
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSTDFIRHGEERLELQGLFALAEDNFACRNA
LLENGIDASDNMVVFERSLFRSGKNSCRINGKLVTTVLLRQIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPAL
TKYQTNFKEFQTIVREWQNWTKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYTAIQG
EPGGLEFIGEAMRQMETAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPDELNQIESRLNDLNQLKRKYGKT
IEDIIQYEQEISREMEKLTNSESHVGHLETKMATLKTELTKQASALTDIRKKAAATLEKQIKQELNQLYMEKAIFSVRFE
SNKTELTESGQDNVVFYMSTNPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTILKGDEKVEEISRMIAGIEVTDLTKQHAKEMIQQAEKVK
QTY
>Mature_563_residues
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSTDFIRHGEERLELQGLFALAEDNFACRNA
LLENGIDASDNMVVFERSLFRSGKNSCRINGKLVTTVLLRQIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPAL
TKYQTNFKEFQTIVREWQNWTKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYTAIQG
EPGGLEFIGEAMRQMETAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPDELNQIESRLNDLNQLKRKYGKT
IEDIIQYEQEISREMEKLTNSESHVGHLETKMATLKTELTKQASALTDIRKKAAATLEKQIKQELNQLYMEKAIFSVRFE
SNKTELTESGQDNVVFYMSTNPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTILKGDEKVEEISRMIAGIEVTDLTKQHAKEMIQQAEKVK
QTY

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=561, Percent_Identity=32.7985739750446, Blast_Score=292, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 63678; Mature: 63678

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSTDFIRHGEE
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHCCHH
RLELQGLFALAEDNFACRNALLENGIDASDNMVVFERSLFRSGKNSCRINGKLVTTVLLR
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCEEECCHHHHHHHHH
QIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPALTKYQTNFKEFQTIVREWQNW
HHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
TKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYTAIQG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
EPGGLEFIGEAMRQMETAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPDEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHH
NQIESRLNDLNQLKRKYGKTIEDIIQYEQEISREMEKLTNSESHVGHLETKMATLKTELT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KQASALTDIRKKAAATLEKQIKQELNQLYMEKAIFSVRFESNKTELTESGQDNVVFYMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHCCCCCCCEEEEEEC
NPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTILKGDEKVEEISRMIAGIE
HHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHCCC
VTDLTKQHAKEMIQQAEKVKQTY
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSTDFIRHGEE
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHCCHH
RLELQGLFALAEDNFACRNALLENGIDASDNMVVFERSLFRSGKNSCRINGKLVTTVLLR
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCEEECCHHHHHHHHH
QIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPALTKYQTNFKEFQTIVREWQNW
HHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
TKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYTAIQG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
EPGGLEFIGEAMRQMETAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPDEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHH
NQIESRLNDLNQLKRKYGKTIEDIIQYEQEISREMEKLTNSESHVGHLETKMATLKTELT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KQASALTDIRKKAAATLEKQIKQELNQLYMEKAIFSVRFESNKTELTESGQDNVVFYMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHCCCCCCCEEEEEEC
NPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTILKGDEKVEEISRMIAGIE
HHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHCCC
VTDLTKQHAKEMIQQAEKVKQTY
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]