Click here to switch to the map view.

The map label for this gene is infB [H]

Identifier: 116872756

GI number: 116872756

Start: 1332537

End: 1334879

Strand: Direct

Name: infB [H]

Synonym: lwe1340

Alternate gene names: 116872756

Gene position: 1332537-1334879 (Clockwise)

Preceding gene: 116872755

Following gene: 116872757

Centisome position: 47.35

GC content: 38.03

Gene sequence:

>2343_bases
ATGAGTAAAGTTCGTGTATATGAATATGCAAAAGAACATCAAGTATCAAGCAAAAAAGTCATTGAAGCATTAAAAGACTT
AGGTATTGAAGTGGCGAATCATATGTCCACTATTAATGAAAATGCCTTAAGACAATTAGATAATGCCGTTGATGGCACAA
ATAAAAAAGCCGAAGCACCAAAGAAAGAAACTACAAGCAACGAAAATGGAAATAGTAAGGGGCCAAACAAACCAAATATG
ACAAATAGTAATGAAAAGTCGAATAAACCAAATAAACCAGCAGGACAAGCTACTAAACCAGCCACTGCAAACAAAAGCCA
AGGTGCAAAACCAGCAACAAACAAACCAGCAAATACAAGTAATCAAACCCAATCAAGTGGTAATCAGCAACAAGCTGGCG
GACAAAAACGTAATAATAATAGCAATCGTCCAGGAGGCGGAAACTCTAACCGTCCAGGCGGTAACAATCGTCCGAATCGT
GGCGGCAATTTCAATAATAAAGGCCGTAATACGAAGAAAAAAGGTAAATTAAACCATAGTACAGTTCCGCCAACTCCGCC
AAAACCAAAAGAACTTCCTGAAAAAATTGTTTTTAGTGAATCATTGACAGTAGCGGAATTAGCGAAAAAATTATACAGAG
AACCATCTGAATTAATTAAAAAATTATTTATGCTTGGTGTTGTAGCGACAATTAATCAATCATTAGATAAAGATGCAATC
GAACTAATCTGTGATGATTATGGTGTGCAGGTAGAAGAAGAAATTAAAGTCGATGTAACGGATTTAGATGTTTACTTTGA
AAACGAATTAAATGAAACGGTTGACGAGTCTAAACTTGTTGAACGTCCACCAGTTGTTACTATTATGGGACACGTTGACC
ATGGTAAAACAACGTTACTAGATTCCCTTCGTAATACAAAAGTTACTTTAGGTGAAGCTGGCGGTATTACGCAACATATT
GGTGCGTATCAACTTGAAATCCATGATAAAAAAATCACTTTCCTAGATACACCTGGACATGCTGCGTTTACAGCAATGCG
TGCTCGTGGTGCGCAAATCACTGATATTACTATTTTAGTAGTTGCAGCAGATGATGGTGTTATGCCACAAACAATTGAAG
CAATTAACCATGCGAAAGCTGCGGGAATGCCGATTATTGTTGCTGTGAATAAAATTGATAAACCACAAGCAAACCCGGAC
CGTGTTATGCAAGAATTAACTGAATATGAATTAGTCCCAGAAGCTTGGGGCGGCGATACTATTTTCGCACCAATTTCAGC
TAAATTTGGTGAAGGTCTTGAAAACTTGTTAGACATGATTTTACTTGTATCTGAAGTAGAAGAATTAAAAGCAAATCCTA
ATCGTCGTGCAATTGGTTCTGTCATTGAAGCAGAACTTGATAAAGGCCGCGGTCCTGTTGCGACTTTATTAGTACAAGAT
GGAACGCTTAATATTGGTGATCCAATTGTTGTCGGTAACACATTTGGACGTGTTCGTGCAATGGTCAATGATTTAGGCCG
TCGTGTGAAAAAAGTGGGTCCTAGTACACCAGTTGAAATCACTGGTTTAAATGATGTACCACAAGCCGGCGATCGCTTTG
TTGTTTTTGAAGATGAAAAAACAGCAAGAAATATTGGTGAAACTCGTGCAAGCCGTGCACTAGTAGCTCAACGTTCAGCA
ACAAACCGTGTAAGTTTAGATAACTTATTTGAACATATGAAAGCTGGCGAAATGAAAGAAGTTAACGTTATTATTAAAGC
TGACGTTCAAGGTTCTGTAGAGGCTCTTGCCGCATCACTTCGCAAAATTGATGTAGAAGGCGTTAACGTTAAAATTATTC
ATACTGCCGTTGGTGCAATCAATGAATCAGATATCACTTTAGCTGCAGCATCTAATGCGATTGTTATTGGATTTAATGTT
CGTCCAACAGCACAAGCTCGTGAAGCAGCAGAAAACGAAAGCGTTGATATTCGTTTACACCGTGTTATTTATAAAGCAAT
TGATGAAATTGAAGCAGCAATGAAAGGGATGCTTGATCCAGAATTCCAAGAAAAAATCATTGGTCAAGCGCAAGTTCGTC
AAACAATCAATGTTTCTAAAGTGGGTACAATTGCCGGTTGTTACGTAACGGATGGTAAAATTACTCGTGATAGTGGCGTT
CGTATTATCCGTGACGGAATCGTTGTTTTTGAAGGCGAAATAGCTACACTGAAACGCTTTAAAGATGATGCGAAAGAAGT
GGCTAAAGGGTACGAATGTGGTATTACAGTTCAAAACTTCAATGATATCAAAGAAGACGATGTAATTGAAGCGTACGTTA
TGGAAGAAATTGAAAGAAAATGA

Upstream 100 bases:

>100_bases
ATCGGCAAAGACACACGTGCAATAGTTGCAATACTTGATAAAGGGTTTGCTGTTAAATTAGCAGAATTACTCGGTTGAAT
CTTATACGGAGGTGTACGAC

Downstream 100 bases:

>100_bases
TTCAATCAGTTGTTAGTGAATTTTTCATGCAGGAACCACAAAACCTCAAAGAAAAACGCGCTATACTGAAACGGATTTTA
ACAAGAGCAAAACAAAAATT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MSKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAVDGTNKKAEAPKKETTSNENGNSKGPNKPNM
TNSNEKSNKPNKPAGQATKPATANKSQGAKPATNKPANTSNQTQSSGNQQQAGGQKRNNNSNRPGGGNSNRPGGNNRPNR
GGNFNNKGRNTKKKGKLNHSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKDAI
ELICDDYGVQVEEEIKVDVTDLDVYFENELNETVDESKLVERPPVVTIMGHVDHGKTTLLDSLRNTKVTLGEAGGITQHI
GAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPD
RVMQELTEYELVPEAWGGDTIFAPISAKFGEGLENLLDMILLVSEVEELKANPNRRAIGSVIEAELDKGRGPVATLLVQD
GTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEKTARNIGETRASRALVAQRSA
TNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNV
RPTAQAREAAENESVDIRLHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDSGV
RIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK

Sequences:

>Translated_780_residues
MSKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAVDGTNKKAEAPKKETTSNENGNSKGPNKPNM
TNSNEKSNKPNKPAGQATKPATANKSQGAKPATNKPANTSNQTQSSGNQQQAGGQKRNNNSNRPGGGNSNRPGGNNRPNR
GGNFNNKGRNTKKKGKLNHSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKDAI
ELICDDYGVQVEEEIKVDVTDLDVYFENELNETVDESKLVERPPVVTIMGHVDHGKTTLLDSLRNTKVTLGEAGGITQHI
GAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPD
RVMQELTEYELVPEAWGGDTIFAPISAKFGEGLENLLDMILLVSEVEELKANPNRRAIGSVIEAELDKGRGPVATLLVQD
GTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEKTARNIGETRASRALVAQRSA
TNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNV
RPTAQAREAAENESVDIRLHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDSGV
RIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK
>Mature_779_residues
SKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAVDGTNKKAEAPKKETTSNENGNSKGPNKPNMT
NSNEKSNKPNKPAGQATKPATANKSQGAKPATNKPANTSNQTQSSGNQQQAGGQKRNNNSNRPGGGNSNRPGGNNRPNRG
GNFNNKGRNTKKKGKLNHSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKDAIE
LICDDYGVQVEEEIKVDVTDLDVYFENELNETVDESKLVERPPVVTIMGHVDHGKTTLLDSLRNTKVTLGEAGGITQHIG
AYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPDR
VMQELTEYELVPEAWGGDTIFAPISAKFGEGLENLLDMILLVSEVEELKANPNRRAIGSVIEAELDKGRGPVATLLVQDG
TLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEKTARNIGETRASRALVAQRSAT
NRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNVR
PTAQAREAAENESVDIRLHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDSGVR
IIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=549, Percent_Identity=39.1621129326047, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI53729337, Length=549, Percent_Identity=39.1621129326047, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI84043963, Length=472, Percent_Identity=28.1779661016949, Blast_Score=134, Evalue=5e-31,
Organism=Homo sapiens, GI157426893, Length=252, Percent_Identity=30.1587301587302, Blast_Score=87, Evalue=7e-17,
Organism=Escherichia coli, GI1789559, Length=667, Percent_Identity=50.0749625187406, Blast_Score=620, Evalue=1e-178,
Organism=Escherichia coli, GI48994988, Length=146, Percent_Identity=35.6164383561644, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI2367247, Length=225, Percent_Identity=31.1111111111111, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1788922, Length=232, Percent_Identity=28.8793103448276, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790412, Length=125, Percent_Identity=37.6, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789737, Length=125, Percent_Identity=37.6, Blast_Score=63, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=536, Percent_Identity=36.5671641791045, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI212656558, Length=561, Percent_Identity=25.3119429590018, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=31.3364055299539, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=248, Percent_Identity=32.6612903225806, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324550, Length=623, Percent_Identity=38.6837881219904, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319282, Length=537, Percent_Identity=25.512104283054, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=238, Percent_Identity=31.9327731092437, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=271, Percent_Identity=25.830258302583, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28572034, Length=531, Percent_Identity=39.3596986817326, Blast_Score=337, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24656849, Length=470, Percent_Identity=28.0851063829787, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI160714833, Length=73, Percent_Identity=72.6027397260274, Blast_Score=115, Evalue=2e-25,
Organism=Drosophila melanogaster, GI78706572, Length=224, Percent_Identity=29.4642857142857, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363316, Length=269, Percent_Identity=31.5985130111524, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17864358, Length=269, Percent_Identity=31.5985130111524, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 84720; Mature: 84589

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAVDGTNKKAEAP
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KKETTSNENGNSKGPNKPNMTNSNEKSNKPNKPAGQATKPATANKSQGAKPATNKPANTS
CHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NQTQSSGNQQQAGGQKRNNNSNRPGGGNSNRPGGNNRPNRGGNFNNKGRNTKKKGKLNHS
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKDAI
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHH
ELICDDYGVQVEEEIKVDVTDLDVYFENELNETVDESKLVERPPVVTIMGHVDHGKTTLL
HHHHHCCCCEEECCEEEEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
DSLRNTKVTLGEAGGITQHIGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILV
HHHCCCEEEEECCCCCHHHCCEEEEEEECCEEEEEECCCCHHHHHHHHCCCEEEEEEEEE
VAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPDRVMQELTEYELVPEAWGGDT
EECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCE
IFAPISAKFGEGLENLLDMILLVSEVEELKANPNRRAIGSVIEAELDKGRGPVATLLVQD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEEC
GTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEK
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECCCH
TARNIGETRASRALVAQRSATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASL
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHH
RKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNVRPTAQAREAAENESVDIRLH
HHEEECCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHCCCCCEEHHHH
RVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDSGV
HHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCHHHCCCEEEEEEECCEEECCCCC
RIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK
EEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAVDGTNKKAEAP
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KKETTSNENGNSKGPNKPNMTNSNEKSNKPNKPAGQATKPATANKSQGAKPATNKPANTS
CHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NQTQSSGNQQQAGGQKRNNNSNRPGGGNSNRPGGNNRPNRGGNFNNKGRNTKKKGKLNHS
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKDAI
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHH
ELICDDYGVQVEEEIKVDVTDLDVYFENELNETVDESKLVERPPVVTIMGHVDHGKTTLL
HHHHHCCCCEEECCEEEEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
DSLRNTKVTLGEAGGITQHIGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILV
HHHCCCEEEEECCCCCHHHCCEEEEEEECCEEEEEECCCCHHHHHHHHCCCEEEEEEEEE
VAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPDRVMQELTEYELVPEAWGGDT
EECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCE
IFAPISAKFGEGLENLLDMILLVSEVEELKANPNRRAIGSVIEAELDKGRGPVATLLVQD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEEEC
GTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEK
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECCCH
TARNIGETRASRALVAQRSATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASL
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHH
RKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNVRPTAQAREAAENESVDIRLH
HHEEECCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHCCCCCEEHHHH
RVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDSGV
HHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCHHHCCCEEEEEEECCEEECCCCC
RIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK
EEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]