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The map label for this gene is proS [H]

Identifier: 116872750

GI number: 116872750

Start: 1323980

End: 1325686

Strand: Direct

Name: proS [H]

Synonym: lwe1334

Alternate gene names: 116872750

Gene position: 1323980-1325686 (Clockwise)

Preceding gene: 116872749

Following gene: 116872751

Centisome position: 47.05

GC content: 38.02

Gene sequence:

>1707_bases
ATGCGTCAAACGATGACATTTATACCAACATTAAAAGAAGTTCCGGCGGATGCGGAAGTAAAGAGCCACCAATTACTTTT
ACGCGCTGGTTTTATAAGACAGACTGCGAGTGGGATTTACAGTTATTTACCACTTGCTACACTGATGTTACGAAAAATCG
AAGCAATCATACGTGAAGAGTTGGAAGCAATAGGAGCAGCAGAATTACTTTTACCAGCACTTCAACCAGCTGAACTATGG
CAAGAGTCTGGTCGTTGGAATGATTATGGTCCAGAATTAATGCGCCTAAAAGACCGCGCTTCTCGTGATTTTGCGCTTGG
ACCAACACATGAAGAAGTTATTACGGCACTTTTACGTGATGAAGTAAAATCATATAAACGTTTACCATTAACTTTGTATC
AAATCCAAACAAAATTCCGTGATGAAAAACGTCCGCGCTTCGGTTTACTACGTGGTCGTGAATTCATTATGAAAGATGCT
TATTCTTTCCATGCTACTAGCGAAAGTTTGGATGAAGTTTACAAGTTAATGCACCAAGCTTATTCTAATATTTTTACTCG
GTGCGGCCTTGAATTCCGTTCTGTTATTGCGGACTCCGGATCTATCGGCGGAAATGAATCAAAAGAATTTATGGCATTAT
CTGAAATTGGTGAAGATACAATTGCTTATAGTGATGCTTCTGATTACGCAGCAAACACTGAAATGGCACCAGTTTTATAT
ATGGAGAAAAAATCTCATGAACTTGAGAAAGAGTTAGAAAAAGTAGCAACTGCCAATCAAAAATTCATTGCTGATATTGT
TGAGTTTTTAGAAGTACCAATTGAAAAAACGATTAAATCAATGCTTTACCAAGTAGACGAAGAAGTAATTATGGTATTAG
TTCGCGGTGATCATGAAGTGAATGATATCAAAATCAAAAACGCGCTTGATGCAACGAATGTAGAGCTAGTTGATCCAGCT
GTTCCAGTGGAAATACTTGGCGCAAACTTTGGTTCCCTCGGTCCAATTGGCGTTCCAGAAAATATTCGTGTATTTGCAGA
TAATGCAGTAAAAGATATTGCTAATGCGGTTGTTGGTGCAAATGAAGATGGCTACCACTATGTTAATGTGAATCCAAATC
GTGATTTTGAAGTTACTAGCTATTTCGATTTACGTATGATTCAAGCTGGTGATTTATCTCCAGATGGTCAAGGTGTCATT
AAATTTGCAGAAGGCATCGAGGTTGGTCATATTTTCAAACTTGGCACAAAATACAGTGAAGCGATGAATGCGACAATTTT
AGATGAAAACGGTCGTGCACAACCAATTATCATGGGTTGTTACGGAATTGGTGTGTCTCGTATTTTATCCGCCATTGCCG
AACAATCAAATGATGAAAATGGCCTAGTATGGGATAAGCAAATTAGCCCATTTGATTTACATTTAATTCCTGTTAATATG
AAGAGTGAAGAGCAAGTTGCTTTTGCAGAATCTCTATATGACTCGTTGCAAAAAGCAGGATTTAGCGTATTAATTGATGA
TCGCGCTGAACGTGCGGGTGTTAAATTTGCAGACGCTGATTTAATCGGTTTACCAATTCGCATTACTGTTGGTAAAAAAG
CAGCAGAAGGTATCGTGGAAGTGAAAATCAGAAAAACGGGCGAAATGATTGAAGTTCGTCAAGATGAATTACTCAATACA
TTACCAATTCTTTTTGGAGATAAATAA

Upstream 100 bases:

>100_bases
GCTTTTAATGGTTCTAATGATTCTTGTGACATGGAACGATATTCAACGAGCATTTTTCTAAAACAGAAAGACTAATAAAG
GGAAAAAAGGGGTGTTTTAA

Downstream 100 bases:

>100_bases
TTTACTGATACATTCGTGCCAGATGAAACTCCACTCGTTCCATAAAAAACGAGTCATGAGGGACATCTGGCTTTTATAAA
TTCAGATAAAGGGGTGTTAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MRQTMTFIPTLKEVPADAEVKSHQLLLRAGFIRQTASGIYSYLPLATLMLRKIEAIIREELEAIGAAELLLPALQPAELW
QESGRWNDYGPELMRLKDRASRDFALGPTHEEVITALLRDEVKSYKRLPLTLYQIQTKFRDEKRPRFGLLRGREFIMKDA
YSFHATSESLDEVYKLMHQAYSNIFTRCGLEFRSVIADSGSIGGNESKEFMALSEIGEDTIAYSDASDYAANTEMAPVLY
MEKKSHELEKELEKVATANQKFIADIVEFLEVPIEKTIKSMLYQVDEEVIMVLVRGDHEVNDIKIKNALDATNVELVDPA
VPVEILGANFGSLGPIGVPENIRVFADNAVKDIANAVVGANEDGYHYVNVNPNRDFEVTSYFDLRMIQAGDLSPDGQGVI
KFAEGIEVGHIFKLGTKYSEAMNATILDENGRAQPIIMGCYGIGVSRILSAIAEQSNDENGLVWDKQISPFDLHLIPVNM
KSEEQVAFAESLYDSLQKAGFSVLIDDRAERAGVKFADADLIGLPIRITVGKKAAEGIVEVKIRKTGEMIEVRQDELLNT
LPILFGDK

Sequences:

>Translated_568_residues
MRQTMTFIPTLKEVPADAEVKSHQLLLRAGFIRQTASGIYSYLPLATLMLRKIEAIIREELEAIGAAELLLPALQPAELW
QESGRWNDYGPELMRLKDRASRDFALGPTHEEVITALLRDEVKSYKRLPLTLYQIQTKFRDEKRPRFGLLRGREFIMKDA
YSFHATSESLDEVYKLMHQAYSNIFTRCGLEFRSVIADSGSIGGNESKEFMALSEIGEDTIAYSDASDYAANTEMAPVLY
MEKKSHELEKELEKVATANQKFIADIVEFLEVPIEKTIKSMLYQVDEEVIMVLVRGDHEVNDIKIKNALDATNVELVDPA
VPVEILGANFGSLGPIGVPENIRVFADNAVKDIANAVVGANEDGYHYVNVNPNRDFEVTSYFDLRMIQAGDLSPDGQGVI
KFAEGIEVGHIFKLGTKYSEAMNATILDENGRAQPIIMGCYGIGVSRILSAIAEQSNDENGLVWDKQISPFDLHLIPVNM
KSEEQVAFAESLYDSLQKAGFSVLIDDRAERAGVKFADADLIGLPIRITVGKKAAEGIVEVKIRKTGEMIEVRQDELLNT
LPILFGDK
>Mature_568_residues
MRQTMTFIPTLKEVPADAEVKSHQLLLRAGFIRQTASGIYSYLPLATLMLRKIEAIIREELEAIGAAELLLPALQPAELW
QESGRWNDYGPELMRLKDRASRDFALGPTHEEVITALLRDEVKSYKRLPLTLYQIQTKFRDEKRPRFGLLRGREFIMKDA
YSFHATSESLDEVYKLMHQAYSNIFTRCGLEFRSVIADSGSIGGNESKEFMALSEIGEDTIAYSDASDYAANTEMAPVLY
MEKKSHELEKELEKVATANQKFIADIVEFLEVPIEKTIKSMLYQVDEEVIMVLVRGDHEVNDIKIKNALDATNVELVDPA
VPVEILGANFGSLGPIGVPENIRVFADNAVKDIANAVVGANEDGYHYVNVNPNRDFEVTSYFDLRMIQAGDLSPDGQGVI
KFAEGIEVGHIFKLGTKYSEAMNATILDENGRAQPIIMGCYGIGVSRILSAIAEQSNDENGLVWDKQISPFDLHLIPVNM
KSEEQVAFAESLYDSLQKAGFSVLIDDRAERAGVKFADADLIGLPIRITVGKKAAEGIVEVKIRKTGEMIEVRQDELLNT
LPILFGDK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=46.0176991150442, Blast_Score=218, Evalue=8e-57,
Organism=Escherichia coli, GI1786392, Length=568, Percent_Identity=49.4718309859155, Blast_Score=558, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI115532348, Length=240, Percent_Identity=36.25, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=36.4705882352941, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=551, Percent_Identity=29.038112522686, Blast_Score=226, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24656200, Length=269, Percent_Identity=34.9442379182156, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63350; Mature: 63350

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTMTFIPTLKEVPADAEVKSHQLLLRAGFIRQTASGIYSYLPLATLMLRKIEAIIREE
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAIGAAELLLPALQPAELWQESGRWNDYGPELMRLKDRASRDFALGPTHEEVITALLRD
HHHHCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EVKSYKRLPLTLYQIQTKFRDEKRPRFGLLRGREFIMKDAYSFHATSESLDEVYKLMHQA
HHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
YSNIFTRCGLEFRSVIADSGSIGGNESKEFMALSEIGEDTIAYSDASDYAANTEMAPVLY
HHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEE
MEKKSHELEKELEKVATANQKFIADIVEFLEVPIEKTIKSMLYQVDEEVIMVLVRGDHEV
EEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHEEEEEECCCCC
NDIKIKNALDATNVELVDPAVPVEILGANFGSLGPIGVPENIRVFADNAVKDIANAVVGA
CCEEEECCCCCCCEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCC
NEDGYHYVNVNPNRDFEVTSYFDLRMIQAGDLSPDGQGVIKFAEGIEVGHIFKLGTKYSE
CCCCEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCEEHHCCCCCCCEEECCCHHHH
AMNATILDENGRAQPIIMGCYGIGVSRILSAIAEQSNDENGLVWDKQISPFDLHLIPVNM
HCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEECC
KSEEQVAFAESLYDSLQKAGFSVLIDDRAERAGVKFADADLIGLPIRITVGKKAAEGIVE
CCCHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCEEECCCEEECEEEEEECCHHHCCEEE
VKIRKTGEMIEVRQDELLNTLPILFGDK
EEEECCCCHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MRQTMTFIPTLKEVPADAEVKSHQLLLRAGFIRQTASGIYSYLPLATLMLRKIEAIIREE
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAIGAAELLLPALQPAELWQESGRWNDYGPELMRLKDRASRDFALGPTHEEVITALLRD
HHHHCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EVKSYKRLPLTLYQIQTKFRDEKRPRFGLLRGREFIMKDAYSFHATSESLDEVYKLMHQA
HHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
YSNIFTRCGLEFRSVIADSGSIGGNESKEFMALSEIGEDTIAYSDASDYAANTEMAPVLY
HHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEE
MEKKSHELEKELEKVATANQKFIADIVEFLEVPIEKTIKSMLYQVDEEVIMVLVRGDHEV
EEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHEEEEEECCCCC
NDIKIKNALDATNVELVDPAVPVEILGANFGSLGPIGVPENIRVFADNAVKDIANAVVGA
CCEEEECCCCCCCEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCC
NEDGYHYVNVNPNRDFEVTSYFDLRMIQAGDLSPDGQGVIKFAEGIEVGHIFKLGTKYSE
CCCCEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCEEHHCCCCCCCEEECCCHHHH
AMNATILDENGRAQPIIMGCYGIGVSRILSAIAEQSNDENGLVWDKQISPFDLHLIPVNM
HCCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEECC
KSEEQVAFAESLYDSLQKAGFSVLIDDRAERAGVKFADADLIGLPIRITVGKKAAEGIVE
CCCHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCEEECCCEEECEEEEEECCHHHCCEEE
VKIRKTGEMIEVRQDELLNTLPILFGDK
EEEECCCCHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]