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The map label for this gene is hemD [H]

Identifier: 116872574

GI number: 116872574

Start: 1166659

End: 1168140

Strand: Direct

Name: hemD [H]

Synonym: lwe1158

Alternate gene names: 116872574

Gene position: 1166659-1168140 (Clockwise)

Preceding gene: 116872573

Following gene: 116872575

Centisome position: 41.46

GC content: 39.41

Gene sequence:

>1482_bases
ATGGGAAAAGTCATGTTAATTGGCGCAGGTCCTGGAGATGCACAACTGCTCACAGTAAAAGGATTAGCCGCACTTCAAAA
AGCGGATGTTATTGTGTATGATCGTCTCGTGGAAAAAGCAATGCTCCAAGAACGAAAAGCGAGCTGCAAACTTATTTATG
TGGGAAAAGAACCGTTACATCATCCAATTCCTCAAGAAGAAATCGAACAAATCCTTGTTAGGGAAGCTGCAACAAATGAG
CTTGTCGTTCGCTTGAAAGCAGGTGATCCATATGTATTTGGACGTGGTGGAGAAGAAGGAGAAACACTTTATAAAGCGGG
AATTCCTTTTGAAGTTATCCCTGGAATCACCTCAGCTATTGGTGGACTTGCTTATGCGGGAATTCCGGTCACACACCGTG
ATTTTGCTTCAAGTTTCCACGTGATTACCGGTCATTTGAAAAAAGGACGGGATCCACTAGACTGGGAAGCTCTTGCTAGG
TTAGAAGGTACACTCGTTTTTCTGATGGGAATGACAAACTTGCCTAATATTTGCGCGAATTTACTAGCAAATGGACAAAA
ACCAGAAACTGGGGTTGCGATTGTGCAATGGGCATCCCGAGGCAAACAATTAACTGTTACTGGAAATTTACAAACTATCG
AGCAAAAAGTAGCAGAGTCCGGTATTTCATCACCTGCGCTTATCGTTGTTGGTGAAGTAGTGAGCTTACGGCCACAGCTT
AACTTTTTTGAAGAAATGCCGCTTTTCCATACCAAAGTCTTACTTCCAAGAGCACGAGCTGGTAAAAGTAAGCTCGCAGA
ACAAATTCGTGATTTAGGCGGAGAAGTAACAATGTATCCAAATATTCAAGTATTGGATGTGACACCAACAAAAACAAAAC
AAGAATTATGCGAAACTCGTGAAATTATTTTTACATCAAAAGAGGCAGTAGAGCGATTTTTTAATTATCTAGCGACGCTT
CATTTAGATATTCGTACTTTGAAAAATACACACCTCACAGCTATTGGCAAACAAACGAAAGAAGCACTCAGCGAAAAAGG
AATTGTTCCAGATAGTTTTATACAAAGAAGAAGTGCTGAGTTGATTTTAGAGCATTTAGCAAGATTTCAAAAAAATTCGA
GCAATTTGATTATTGGGAGTGTAGTTGACACAGCAGAAGTTGGCGCTATTTTGTCTAATGTAAAGGCAATAGAAATGATG
CCTTTATACGATTTGCGACCAGTGACAGAACGTCCATTTGATCTTGAAAGCTTTGAACAAATTTGTTTTTCAAGTTCTCG
TTCGGTCCAAAATCTACTCGATGTATTAACAACCGAAGAAAAAGCAATTTTAAAAACGAAAACCATCCTTTCCATTGGAC
GATTCACGAGCGCAACCTTGCAGTCATTTGGTATTAATGATTTTGAAGAAGCTGATAGTGCAACACCAGAGAGCTTAATT
GAATTAATCCAAAAAACAAAATTAGAAGGAGTACCACAATGA

Upstream 100 bases:

>100_bases
TTCCTGTCATCGTGATTCGCCGCAAACAATTAAATTATCCAATCGAAATAAATCATTTAACCGAACTACCAGAAATTTTA
ACTCAATTGGAGGTCTACTA

Downstream 100 bases:

>100_bases
AAAAAGCCATATTAGTTGTTAGTTTTGGCACTAGTTATCCAGAAACAAGAGAAAAAACCATTGAAGCCTGTGAAAAAAGA
GTAGCACAAGAATTTCCAGA

Product: uroporphyrinogen III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEKAMLQERKASCKLIYVGKEPLHHPIPQEEIEQILVREAATNE
LVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAIGGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALAR
LEGTLVFLMGMTNLPNICANLLANGQKPETGVAIVQWASRGKQLTVTGNLQTIEQKVAESGISSPALIVVGEVVSLRPQL
NFFEEMPLFHTKVLLPRARAGKSKLAEQIRDLGGEVTMYPNIQVLDVTPTKTKQELCETREIIFTSKEAVERFFNYLATL
HLDIRTLKNTHLTAIGKQTKEALSEKGIVPDSFIQRRSAELILEHLARFQKNSSNLIIGSVVDTAEVGAILSNVKAIEMM
PLYDLRPVTERPFDLESFEQICFSSSRSVQNLLDVLTTEEKAILKTKTILSIGRFTSATLQSFGINDFEEADSATPESLI
ELIQKTKLEGVPQ

Sequences:

>Translated_493_residues
MGKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEKAMLQERKASCKLIYVGKEPLHHPIPQEEIEQILVREAATNE
LVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAIGGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALAR
LEGTLVFLMGMTNLPNICANLLANGQKPETGVAIVQWASRGKQLTVTGNLQTIEQKVAESGISSPALIVVGEVVSLRPQL
NFFEEMPLFHTKVLLPRARAGKSKLAEQIRDLGGEVTMYPNIQVLDVTPTKTKQELCETREIIFTSKEAVERFFNYLATL
HLDIRTLKNTHLTAIGKQTKEALSEKGIVPDSFIQRRSAELILEHLARFQKNSSNLIIGSVVDTAEVGAILSNVKAIEMM
PLYDLRPVTERPFDLESFEQICFSSSRSVQNLLDVLTTEEKAILKTKTILSIGRFTSATLQSFGINDFEEADSATPESLI
ELIQKTKLEGVPQ
>Mature_492_residues
GKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEKAMLQERKASCKLIYVGKEPLHHPIPQEEIEQILVREAATNEL
VVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAIGGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALARL
EGTLVFLMGMTNLPNICANLLANGQKPETGVAIVQWASRGKQLTVTGNLQTIEQKVAESGISSPALIVVGEVVSLRPQLN
FFEEMPLFHTKVLLPRARAGKSKLAEQIRDLGGEVTMYPNIQVLDVTPTKTKQELCETREIIFTSKEAVERFFNYLATLH
LDIRTLKNTHLTAIGKQTKEALSEKGIVPDSFIQRRSAELILEHLARFQKNSSNLIIGSVVDTAEVGAILSNVKAIEMMP
LYDLRPVTERPFDLESFEQICFSSSRSVQNLLDVLTTEEKAILKTKTILSIGRFTSATLQSFGINDFEEADSATPESLIE
LIQKTKLEGVPQ

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=51.2396694214876, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=33.6170212765957, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54179; Mature: 54048

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEKAMLQERKASCKLIYVGKEPLH
CCCEEEEECCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
HPIPQEEIEQILVREAATNELVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAI
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCEEEECCCCHHHHCCHHHHH
GGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALARLEGTLVFLMGMTNLPNICAN
HHHHHCCCCCCHHHHHCCHHHEEEHHHCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHH
LLANGQKPETGVAIVQWASRGKQLTVTGNLQTIEQKVAESGISSPALIVVGEVVSLRPQL
HHCCCCCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCC
NFFEEMPLFHTKVLLPRARAGKSKLAEQIRDLGGEVTMYPNIQVLDVTPTKTKQELCETR
CHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHHHHHHH
EIIFTSKEAVERFFNYLATLHLDIRTLKNTHLTAIGKQTKEALSEKGIVPDSFIQRRSAE
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LILEHLARFQKNSSNLIIGSVVDTAEVGAILSNVKAIEMMPLYDLRPVTERPFDLESFEQ
HHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCHHHHHH
ICFSSSRSVQNLLDVLTTEEKAILKTKTILSIGRFTSATLQSFGINDFEEADSATPESLI
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHH
ELIQKTKLEGVPQ
HHHHHHCCCCCCC
>Mature Secondary Structure 
GKVMLIGAGPGDAQLLTVKGLAALQKADVIVYDRLVEKAMLQERKASCKLIYVGKEPLH
CCEEEEECCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
HPIPQEEIEQILVREAATNELVVRLKAGDPYVFGRGGEEGETLYKAGIPFEVIPGITSAI
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCEEEECCCCHHHHCCHHHHH
GGLAYAGIPVTHRDFASSFHVITGHLKKGRDPLDWEALARLEGTLVFLMGMTNLPNICAN
HHHHHCCCCCCHHHHHCCHHHEEEHHHCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHH
LLANGQKPETGVAIVQWASRGKQLTVTGNLQTIEQKVAESGISSPALIVVGEVVSLRPQL
HHCCCCCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCC
NFFEEMPLFHTKVLLPRARAGKSKLAEQIRDLGGEVTMYPNIQVLDVTPTKTKQELCETR
CHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHHHHHHH
EIIFTSKEAVERFFNYLATLHLDIRTLKNTHLTAIGKQTKEALSEKGIVPDSFIQRRSAE
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LILEHLARFQKNSSNLIIGSVVDTAEVGAILSNVKAIEMMPLYDLRPVTERPFDLESFEQ
HHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCHHHHHH
ICFSSSRSVQNLLDVLTTEEKAILKTKTILSIGRFTSATLQSFGINDFEEADSATPESLI
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHH
ELIQKTKLEGVPQ
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]