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The map label for this gene is pduQ [H]

Identifier: 116872545

GI number: 116872545

Start: 1141691

End: 1142830

Strand: Direct

Name: pduQ [H]

Synonym: lwe1129

Alternate gene names: 116872545

Gene position: 1141691-1142830 (Clockwise)

Preceding gene: 116872544

Following gene: 116872546

Centisome position: 40.57

GC content: 37.72

Gene sequence:

>1140_bases
ATGCAAAAAGTTAGTTTTAAAACGGACCTTTACATTGGCCAAGGAGCAACAGATCGTTTACTGGAATTTAAAGAAAAGCA
AATTTTTATTGTAACAGATCCATTTATGGTTAGTTCAGGAATGATTAATGCCATTACAGAAAAAATTGATAAGTCGAATA
CATATACGATTTTTAGCGATATTATTCCAGATCCACCGATTGAAAATGTTGTAGCAGGCATTGAAGTTTTAAATGAATGT
GATGCTAACTTGATGATTGCTATCGGTGGGGGTTCTGCCATTGATGCGGCGAAAGCAATGAAATTTTTTGGTCAAAAACT
TGGAACGGTGCGTGCGATGCCATTCATCGTTATCCCAACTACAAGTGGAACTGGTTCGGAAGTTACTAGTTTTTCTGTCA
TCACAAATAAAGAAAAAGCCATTAAATATCCTCTTATTACGGATGCTATTTTGCCAGATGAAGCGATTTTGGATGCGGAT
TTAGTGAAATCTGTACCGCCAGCAATCACCGCGGATACCGGCATGGACGTACTTACGCATGCACTGGAAGCTTATGTATC
TACCAAAGCCAATGATTATTCAGATGCAATGGCTGAAAAAGTTATCCAATTAGTATTCACATACTTAGAACGCGCATATA
AAGATGGAAATGACTTAGAAGCACGCGAAAAAATGCATAATGCTTCTTGTCTTGCAGGAATGGCGTTTAATATCACCTCA
CTAGGATTAAATCATGGTATTGCGCATACAGCAGGTGCTAAATTTAAGATTCCACATGGCCGTATGAACACGCTTCTTTT
ACCACATGTTATTAGTTATAATGCTGGATTAACAAGTGATTTTGGTAACAATCCGGACAATCGTGCTGCAGAACGTTATA
CAGCTATTGCTAAATTACTGAAAATGCCAGCTTCTAATACAAGACTTGGTGTGCGTAGTTTAATTAATGCCATCAAACAA
TTGCAAAAGAAACTCAATATGCCAACAACTTTATCAGAATGTGGTGTAAGTCGTACTGATTTAAACGAGCATATCGCGCA
AATTGCTGAGGGCGCGTTAAATGATGGCTGTACAGCAACGAATCCAAGAACACCAACAGAAACAGATGTTAGTGCTATAC
TTGAAAAAATGTTAGCTTAA

Upstream 100 bases:

>100_bases
AGGCGTACTGTTTACTTAGCAAAGAAGCTTTGAGTTAGAAGAGGAAATTTCTCTACTCAAAGCTTCTTTTTTTAGTTTTA
AAAAATTAGGAGGTAATCTC

Downstream 100 bases:

>100_bases
AAGTTATTTATTTCACAACCGATATTAAAAACCTATTGACATCGTTTTCATGCTACCTTATAATAGAACCGAGCACAAAG
ACGTGTGGAATAATAAATAT

Product: iron-containing alcohol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MQKVSFKTDLYIGQGATDRLLEFKEKQIFIVTDPFMVSSGMINAITEKIDKSNTYTIFSDIIPDPPIENVVAGIEVLNEC
DANLMIAIGGGSAIDAAKAMKFFGQKLGTVRAMPFIVIPTTSGTGSEVTSFSVITNKEKAIKYPLITDAILPDEAILDAD
LVKSVPPAITADTGMDVLTHALEAYVSTKANDYSDAMAEKVIQLVFTYLERAYKDGNDLEAREKMHNASCLAGMAFNITS
LGLNHGIAHTAGAKFKIPHGRMNTLLLPHVISYNAGLTSDFGNNPDNRAAERYTAIAKLLKMPASNTRLGVRSLINAIKQ
LQKKLNMPTTLSECGVSRTDLNEHIAQIAEGALNDGCTATNPRTPTETDVSAILEKMLA

Sequences:

>Translated_379_residues
MQKVSFKTDLYIGQGATDRLLEFKEKQIFIVTDPFMVSSGMINAITEKIDKSNTYTIFSDIIPDPPIENVVAGIEVLNEC
DANLMIAIGGGSAIDAAKAMKFFGQKLGTVRAMPFIVIPTTSGTGSEVTSFSVITNKEKAIKYPLITDAILPDEAILDAD
LVKSVPPAITADTGMDVLTHALEAYVSTKANDYSDAMAEKVIQLVFTYLERAYKDGNDLEAREKMHNASCLAGMAFNITS
LGLNHGIAHTAGAKFKIPHGRMNTLLLPHVISYNAGLTSDFGNNPDNRAAERYTAIAKLLKMPASNTRLGVRSLINAIKQ
LQKKLNMPTTLSECGVSRTDLNEHIAQIAEGALNDGCTATNPRTPTETDVSAILEKMLA
>Mature_379_residues
MQKVSFKTDLYIGQGATDRLLEFKEKQIFIVTDPFMVSSGMINAITEKIDKSNTYTIFSDIIPDPPIENVVAGIEVLNEC
DANLMIAIGGGSAIDAAKAMKFFGQKLGTVRAMPFIVIPTTSGTGSEVTSFSVITNKEKAIKYPLITDAILPDEAILDAD
LVKSVPPAITADTGMDVLTHALEAYVSTKANDYSDAMAEKVIQLVFTYLERAYKDGNDLEAREKMHNASCLAGMAFNITS
LGLNHGIAHTAGAKFKIPHGRMNTLLLPHVISYNAGLTSDFGNNPDNRAAERYTAIAKLLKMPASNTRLGVRSLINAIKQ
LQKKLNMPTTLSECGVSRTDLNEHIAQIAEGALNDGCTATNPRTPTETDVSAILEKMLA

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=403, Percent_Identity=24.317617866005, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1787493, Length=391, Percent_Identity=35.2941176470588, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI48994951, Length=370, Percent_Identity=34.8648648648649, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87082107, Length=364, Percent_Identity=35.989010989011, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789163, Length=380, Percent_Identity=33.6842105263158, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789386, Length=316, Percent_Identity=19.9367088607595, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17537053, Length=370, Percent_Identity=25.4054054054054, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=398, Percent_Identity=36.9346733668342, Blast_Score=222, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24657991, Length=407, Percent_Identity=23.8329238329238, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 40904; Mature: 40904

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVSFKTDLYIGQGATDRLLEFKEKQIFIVTDPFMVSSGMINAITEKIDKSNTYTIFSD
CCCCCCCCCEEECCCCHHHHHHHCCCEEEEEECCHHHHCCHHHHHHHHHCCCCCEEEECC
IIPDPPIENVVAGIEVLNECDANLMIAIGGGSAIDAAKAMKFFGQKLGTVRAMPFIVIPT
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEC
TSGTGSEVTSFSVITNKEKAIKYPLITDAILPDEAILDADLVKSVPPAITADTGMDVLTH
CCCCCCCCEEEEEEECCCHHEECCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
ALEAYVSTKANDYSDAMAEKVIQLVFTYLERAYKDGNDLEAREKMHNASCLAGMAFNITS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHH
LGLNHGIAHTAGAKFKIPHGRMNTLLLPHVISYNAGLTSDFGNNPDNRAAERYTAIAKLL
HHHCCCCHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KMPASNTRLGVRSLINAIKQLQKKLNMPTTLSECGVSRTDLNEHIAQIAEGALNDGCTAT
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
NPRTPTETDVSAILEKMLA
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQKVSFKTDLYIGQGATDRLLEFKEKQIFIVTDPFMVSSGMINAITEKIDKSNTYTIFSD
CCCCCCCCCEEECCCCHHHHHHHCCCEEEEEECCHHHHCCHHHHHHHHHCCCCCEEEECC
IIPDPPIENVVAGIEVLNECDANLMIAIGGGSAIDAAKAMKFFGQKLGTVRAMPFIVIPT
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEC
TSGTGSEVTSFSVITNKEKAIKYPLITDAILPDEAILDADLVKSVPPAITADTGMDVLTH
CCCCCCCCEEEEEEECCCHHEECCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
ALEAYVSTKANDYSDAMAEKVIQLVFTYLERAYKDGNDLEAREKMHNASCLAGMAFNITS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHH
LGLNHGIAHTAGAKFKIPHGRMNTLLLPHVISYNAGLTSDFGNNPDNRAAERYTAIAKLL
HHHCCCCHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KMPASNTRLGVRSLINAIKQLQKKLNMPTTLSECGVSRTDLNEHIAQIAEGALNDGCTAT
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
NPRTPTETDVSAILEKMLA
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]