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The map label for this gene is nadE [H]

Identifier: 116872484

GI number: 116872484

Start: 1090221

End: 1091045

Strand: Direct

Name: nadE [H]

Synonym: lwe1068

Alternate gene names: 116872484

Gene position: 1090221-1091045 (Clockwise)

Preceding gene: 116872483

Following gene: 116872485

Centisome position: 38.74

GC content: 42.06

Gene sequence:

>825_bases
ATGGAAATCAGAAAAAGAATTTTAGCAGATATGCAAGTAGCAGAAACGATTGATGCACACGAAGAAATTAGAAAAAGTGT
CGAGTTTTTAAAAGCATATTTAAAAAAGAATACGTTCTTAAAAAGCTTTGTTCTTGGGATTTCTGGGGGGCAAGATTCGA
CTTTAACAGGAAAGTTAGCACAGTTGGCGATAAGTGAAATGCGGGATGAAACAGGTGACAATGAATACCAGTTCTTCGCT
GTGAGCTTGCCATACGGAACACAACTAGATGAATCGGACCGTCAAGATGCACTGAACTTTATGAATCCAGATAATCGCTT
AACTGTGAACATTAAAGCTTCGGTTGACGCTAGTGTCGCGGCACTTGCGGAAGCGGGAGTTGAACTATCTGATTTCGCTA
AAGGAAATGAAAAAGCGCGCGAACGGATGAAAGTACAATATGCGATTGCTGCGATGCATAAAGGCGTAGTGGTTGGAACC
GATCACTCCGCAGAAGCAGTTACTGGCTTTTATACGAAATACGGCGACGGGGGAACGGATATTAATCCACTGTTCCGTTT
GAATAAAAGACAAGGCAAGGCGCTTCTTAAAGAGCTTGGCTGCCCAGAACATTTATATTTGAAAAAACCGACTGCTGATT
TAGAAGATAACAAGCCGGCACTTCCTGACGAGGTCGCGCTTGGTGTGACTTATGACCAAATTGACGATTACTTGGAAGGC
AAAGAAGTCCCAGCAGATGCTGCGGCAAAAATTGAGAACTGGTTTATTAAAACCGAACATAAACGCCATATGGCAATTAC
GATATTTGACGATTTCTGGAAATAG

Upstream 100 bases:

>100_bases
AATGTTGGAAAAATAAGATGCGTAATATCGAAAAAGTACGTAAAAGTGTCCAACTTCATTCACCAGTTGAACTAGATATG
CCGTTTTAGGAGGAATTATA

Downstream 100 bases:

>100_bases
GAGGCTGTTTAAATGAATGTTGGTTATGGTTTATTAACTGCATTTTATACACATCCAGGAGAAAAAGACAATTTAGTTAA
AATTTTGCTTGAAGCGGCAG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEIRKRILADMQVAETIDAHEEIRKSVEFLKAYLKKNTFLKSFVLGISGGQDSTLTGKLAQLAISEMRDETGDNEYQFFA
VSLPYGTQLDESDRQDALNFMNPDNRLTVNIKASVDASVAALAEAGVELSDFAKGNEKARERMKVQYAIAAMHKGVVVGT
DHSAEAVTGFYTKYGDGGTDINPLFRLNKRQGKALLKELGCPEHLYLKKPTADLEDNKPALPDEVALGVTYDQIDDYLEG
KEVPADAAAKIENWFIKTEHKRHMAITIFDDFWK

Sequences:

>Translated_274_residues
MEIRKRILADMQVAETIDAHEEIRKSVEFLKAYLKKNTFLKSFVLGISGGQDSTLTGKLAQLAISEMRDETGDNEYQFFA
VSLPYGTQLDESDRQDALNFMNPDNRLTVNIKASVDASVAALAEAGVELSDFAKGNEKARERMKVQYAIAAMHKGVVVGT
DHSAEAVTGFYTKYGDGGTDINPLFRLNKRQGKALLKELGCPEHLYLKKPTADLEDNKPALPDEVALGVTYDQIDDYLEG
KEVPADAAAKIENWFIKTEHKRHMAITIFDDFWK
>Mature_274_residues
MEIRKRILADMQVAETIDAHEEIRKSVEFLKAYLKKNTFLKSFVLGISGGQDSTLTGKLAQLAISEMRDETGDNEYQFFA
VSLPYGTQLDESDRQDALNFMNPDNRLTVNIKASVDASVAALAEAGVELSDFAKGNEKARERMKVQYAIAAMHKGVVVGT
DHSAEAVTGFYTKYGDGGTDINPLFRLNKRQGKALLKELGCPEHLYLKKPTADLEDNKPALPDEVALGVTYDQIDDYLEG
KEVPADAAAKIENWFIKTEHKRHMAITIFDDFWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=68.2481751824817, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30570; Mature: 30570

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRKRILADMQVAETIDAHEEIRKSVEFLKAYLKKNTFLKSFVLGISGGQDSTLTGKLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
QLAISEMRDETGDNEYQFFAVSLPYGTQLDESDRQDALNFMNPDNRLTVNIKASVDASVA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCHHHH
ALAEAGVELSDFAKGNEKARERMKVQYAIAAMHKGVVVGTDHSAEAVTGFYTKYGDGGTD
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCC
INPLFRLNKRQGKALLKELGCPEHLYLKKPTADLEDNKPALPDEVALGVTYDQIDDYLEG
CCHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHEECCCHHHHHHHHCC
KEVPADAAAKIENWFIKTEHKRHMAITIFDDFWK
CCCCCHHHHHHHHHEEEECCCCEEEEEEECCCCC
>Mature Secondary Structure
MEIRKRILADMQVAETIDAHEEIRKSVEFLKAYLKKNTFLKSFVLGISGGQDSTLTGKLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
QLAISEMRDETGDNEYQFFAVSLPYGTQLDESDRQDALNFMNPDNRLTVNIKASVDASVA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCHHHH
ALAEAGVELSDFAKGNEKARERMKVQYAIAAMHKGVVVGTDHSAEAVTGFYTKYGDGGTD
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCC
INPLFRLNKRQGKALLKELGCPEHLYLKKPTADLEDNKPALPDEVALGVTYDQIDDYLEG
CCHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHEECCCHHHHHHHHCC
KEVPADAAAKIENWFIKTEHKRHMAITIFDDFWK
CCCCCHHHHHHHHHEEEECCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]