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The map label for this gene is hflB [C]

Identifier: 116872192

GI number: 116872192

Start: 800449

End: 801489

Strand: Direct

Name: hflB [C]

Synonym: lwe0772

Alternate gene names: 116872192

Gene position: 800449-801489 (Clockwise)

Preceding gene: 116872190

Following gene: 116872193

Centisome position: 28.44

GC content: 32.08

Gene sequence:

>1041_bases
ATGAAAAAGAAAAATATTTTGAATTTAATTAGATATTACTCAGAAAAAAATGATTTACAATTCAGAAATGAGGCAATAGA
AATTGCACGATATTTTGATCAGAATGGTGATCATCAACTTTCAGAATATATCATGAGTTTACTATCTGATGTAAATACAT
TTGTTCCACAAAGTGATTCTTTTGAAAGTTCTTTTTTCAAAGAGTTGAGTATTAATTCTGAGCCGCTGCCACTTCCAAAG
CAAATAGCTGATGATATCAAAGGTATTATCAATGCTGTTAATCATCATGTCGGTATAAATAAATTTTTGTTTGAAGGAGC
ACCTGGAACAGGTAAAACTGAAAGTGTCAAGCAGATAGCTAGGCTTTTAGATCGACGTTTATACATGGTTGAAATTAGTG
AGTTGATTGATAGTAAAATGGGACAGACCAGCAAGAATATGGTGACTATTTTTAATGATATCAATGAGTTACCGCACCCT
TCAAAAGTACTAATACTTTTTGATGAAATAGATGCAATTGCTTTAGATAGAGTTAATTCTAATGATGTGAGAGAAATGGG
AAGAGTTACATCTACGATGCTGAGAGAATTGGATAAATTAAACGAAGAAGTTGTCCTTATCGCAACCACCAATTTATATC
AAAATTTCGATAAAGCACTTACTAGAAGGTTTGACTCTACTATAAATTTCGATCGATATGATAGGGAAGATTTGATTGAG
ATTGCTGAAAGCATACTCAATACATTATTGAAAAAATTCAAAAGTGCTGGAAGAGATATGAAGTTATTCAAAAAAATTAT
TGGAAATATGGAGACTATTCCTAACCCAGGAGAATTAAAAAATCTTATAAAAGTCTCATTAGCATTTAGTGACCCAAATA
ATGACTTTGATTATCTAAGACGGTTGCTTCAAACAACGCAAAACCTTTCTGAAGAAAATGAATTAAATGAATTAAAAAAA
ATGGGCTTTACAGTGAGAGAAATCGAGCTACTGACGGGCGTTTCTAAAAGTCAAGTTGCGAGAGAGCTGAGGGGAGAATA
G

Upstream 100 bases:

>100_bases
AATTTTGAACACCACTCCATTTGTTTTAATCGTTTCTGTCCCAAAATGTTTATTTTTGTCCCTGTTTATGGTATAGTGTT
GTTGGAAGGGAGTGAGTACT

Downstream 100 bases:

>100_bases
ACAGATGAATGATATTTTGAGTTTAAAAGGTAGTTTTGTACAAAAATCAAATGATAGTAGACCTGGTTCACCAAAATTAC
CAGCAGGTTCAAGTGTGTCA

Product: AAA ATPase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKKKNILNLIRYYSEKNDLQFRNEAIEIARYFDQNGDHQLSEYIMSLLSDVNTFVPQSDSFESSFFKELSINSEPLPLPK
QIADDIKGIINAVNHHVGINKFLFEGAPGTGKTESVKQIARLLDRRLYMVEISELIDSKMGQTSKNMVTIFNDINELPHP
SKVLILFDEIDAIALDRVNSNDVREMGRVTSTMLRELDKLNEEVVLIATTNLYQNFDKALTRRFDSTINFDRYDREDLIE
IAESILNTLLKKFKSAGRDMKLFKKIIGNMETIPNPGELKNLIKVSLAFSDPNNDFDYLRRLLQTTQNLSEENELNELKK
MGFTVREIELLTGVSKSQVARELRGE

Sequences:

>Translated_346_residues
MKKKNILNLIRYYSEKNDLQFRNEAIEIARYFDQNGDHQLSEYIMSLLSDVNTFVPQSDSFESSFFKELSINSEPLPLPK
QIADDIKGIINAVNHHVGINKFLFEGAPGTGKTESVKQIARLLDRRLYMVEISELIDSKMGQTSKNMVTIFNDINELPHP
SKVLILFDEIDAIALDRVNSNDVREMGRVTSTMLRELDKLNEEVVLIATTNLYQNFDKALTRRFDSTINFDRYDREDLIE
IAESILNTLLKKFKSAGRDMKLFKKIIGNMETIPNPGELKNLIKVSLAFSDPNNDFDYLRRLLQTTQNLSEENELNELKK
MGFTVREIELLTGVSKSQVARELRGE
>Mature_346_residues
MKKKNILNLIRYYSEKNDLQFRNEAIEIARYFDQNGDHQLSEYIMSLLSDVNTFVPQSDSFESSFFKELSINSEPLPLPK
QIADDIKGIINAVNHHVGINKFLFEGAPGTGKTESVKQIARLLDRRLYMVEISELIDSKMGQTSKNMVTIFNDINELPHP
SKVLILFDEIDAIALDRVNSNDVREMGRVTSTMLRELDKLNEEVVLIATTNLYQNFDKALTRRFDSTINFDRYDREDLIE
IAESILNTLLKKFKSAGRDMKLFKKIIGNMETIPNPGELKNLIKVSLAFSDPNNDFDYLRRLLQTTQNLSEENELNELKK
MGFTVREIELLTGVSKSQVARELRGE

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324000, Length=129, Percent_Identity=36.4341085271318, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323429, Length=131, Percent_Identity=32.824427480916, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI9755327, Length=195, Percent_Identity=27.1794871794872, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 39797; Mature: 39797

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKNILNLIRYYSEKNDLQFRNEAIEIARYFDQNGDHQLSEYIMSLLSDVNTFVPQSDS
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
FESSFFKELSINSEPLPLPKQIADDIKGIINAVNHHVGINKFLFEGAPGTGKTESVKQIA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHH
RLLDRRLYMVEISELIDSKMGQTSKNMVTIFNDINELPHPSKVLILFDEIDAIALDRVNS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCCCC
NDVREMGRVTSTMLRELDKLNEEVVLIATTNLYQNFDKALTRRFDSTINFDRYDREDLIE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IAESILNTLLKKFKSAGRDMKLFKKIIGNMETIPNPGELKNLIKVSLAFSDPNNDFDYLR
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHH
RLLQTTQNLSEENELNELKKMGFTVREIELLTGVSKSQVARELRGE
HHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MKKKNILNLIRYYSEKNDLQFRNEAIEIARYFDQNGDHQLSEYIMSLLSDVNTFVPQSDS
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
FESSFFKELSINSEPLPLPKQIADDIKGIINAVNHHVGINKFLFEGAPGTGKTESVKQIA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHH
RLLDRRLYMVEISELIDSKMGQTSKNMVTIFNDINELPHPSKVLILFDEIDAIALDRVNS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCCCC
NDVREMGRVTSTMLRELDKLNEEVVLIATTNLYQNFDKALTRRFDSTINFDRYDREDLIE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IAESILNTLLKKFKSAGRDMKLFKKIIGNMETIPNPGELKNLIKVSLAFSDPNNDFDYLR
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHH
RLLQTTQNLSEENELNELKKMGFTVREIELLTGVSKSQVARELRGE
HHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]